A5048960816- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- HIV Research and Treatment
- Dermatology and Skin Diseases
- Urticaria and Related Conditions
- Autoimmune Bullous Skin Diseases
- T-cell and B-cell Immunology
- Cutaneous lymphoproliferative disorders research
- Herpesvirus Infections and Treatments
- Viral-associated cancers and disorders
- Skin and Cellular Biology Research
- Trace Elements in Health
- Cytomegalovirus and herpesvirus research
- RNA Interference and Gene Delivery
- Nonmelanoma Skin Cancer Studies
- Mast cells and histamine
- Drug-Induced Adverse Reactions
- Immune cells in cancer
- Cancer and Skin Lesions
- Vascular Tumors and Angiosarcomas
- Lymphoma Diagnosis and Treatment
- Psoriasis: Treatment and Pathogenesis
- Eosinophilic Disorders and Syndromes
University of Yamanashi
2016-2025
University of Yamanashi Hospital
2005-2022
National Cancer Institute
1999-2004
Center for Cancer Research
2003-2004
Case Western Reserve University
2003
University Hospitals of Cleveland
2003
George Washington University
2003
National Institutes of Health
1999-2002
Juntendo University
1998-2000
National Cancer Institute
1999-2000
ABSTRACT Continual human immunodeficiency virus type 1 (HIV-1) evolution and expansion within the population have led to unequal distribution of HIV-1 group M subtypes. In particular, recent outgrowth subtype C in southern Africa, India, China has fueled speculation that isolates may be more fit vivo. this study, nine B six were added peripheral blood mononuclear cell cultures for a complete pairwise competition experiment. All less than ( P < 0.0001), but intrasubtype variations fitness...
Langerhans cells (LCs) are suspected to be initial targets for HIV after sexual exposure (by becoming infected or by capturing virus). Here, productive R5 infection of LC ex vivo and LC-mediated transmission virus CD4 + T were both found depend on CCR5. By contrast, monocyte-derived dendritic transfer from mediated CCR5-dependent as well DC-specific ICAM-3-grabbing nonintegrin-dependent pathways. Furthermore, in 62 healthy individuals, levels LCs associated with CCR5 genotype. Specifically,...
Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are life-threatening mucocutaneous adverse drug reactions characterized by massive detachment. Cytotoxic T cells associated effector molecules known to drive SJS/TEN pathophysiology, but the contribution of innate immune responses is not well understood. We describe a mechanism which neutrophils triggered inflammation during early phases SJS/TEN. Skin-infiltrating CD8+ produced lipocalin-2 in drug-specific manner, formation...
The lone CX3C chemokine, fractalkine (FK), is expressed in a membrane-bound form on activated endothelial cells and mediates attachment firm adhesion of T cells, monocytes NK cells. We now show that FK associated with dendritic (DC) epidermis lymphoid organs. In normal human skin, dual-color fluorescence microscopy co-localized expression Langerhans expressing CD1a. tonsil, FK-positive DC CD83, marker for mature DC. Human murine cultured up-regulated mRNA maturation. Furthermore, CD40...
Initial biologic events that underlie sexual transmission of HIV-1 are poorly understood. To model these events, we exposed human immature Langerhans cells (LCs) within epithelial tissue explants to two primary and laboratory-adapted isolates. We detected HIV-1Ba-L infection in single LCs spontaneously emigrated from by flow cytometry (median infected = 0.52%, range 0.08–4.77%). HIV-1–infected downregulated surface CD4 CD83, whereas MHC class II, CD80, CD86 were unchanged. For all strains...
Abstract Dendritic cells (DCs) are potent antigen‐presenting that play a pivotal role in regulating immune responses cancer and have recently been shown to be activated by heat shock proteins (HSPs). We previously reported HSP70 expression after hyperthermia induces antitumor immunity. Our system using magnetite cationic liposomes (MCLs) induced necrotic cell death was correlated with release. In the present study, we investigated therapeutic effects of DC therapy combined MCL‐induced on...
Zinc deficiency can be an inherited disorder, in which case it is known as acrodermatitis enteropathica (AE), or acquired disorder caused by low dietary intake of zinc. Even though zinc diminishes cellular and humoral immunity, patients develop immunostimulating skin inflammation. Here, we have demonstrated that despite diminished allergic contact dermatitis mice fed a zinc-deficient (ZD) diet, irritant (ICD) these was more severe prolonged than controls. Further, histological examination...
Background Patients with cancer benefit from treatment immune checkpoint inhibitors (ICIs), and those an inflamed tumor microenvironment (TME) and/or high mutation burden (TMB), particularly, tend to respond ICIs; however, some patients fail, whereas others acquire resistance after initial response despite the TME TMB. We assessed detailed biological mechanisms of ICIs such as programmed death 1 cytotoxic T-lymphocyte-associated protein 4 blockade therapies using clinical samples. Methods...
Abstract Epidermal Langerhans cells (LC) are Ia‐bearing potent antigen‐presenting (APC) of dendritic cell lineage that play a crucial role in primary and secondary T cell‐dependent immune responses. LC express several costimulatory molecules such as B7, which has been implicated one the important determinants professional APC. Recently, B7 antigens have shown to include three distinct termed B7‐1, B7‐2, B7‐3, expression B7‐1 B7‐2 already confirmed. However, little is known regulation LC. We...
Malignancy has been reported as a causative factor of cutaneous vasculitis, although only two retrospective epidemiological studies have analysed the association between Henoch-Schönlein purpura (HSP) and malignancy to date.To analyse adult HSP malignancy.We retrospectively reviewed medical records patients found 103 cases over past 20 years. Fifty-three (aged > or = 41 years) were categorized groups including 'with malignancy' 'without malignancy', so that we could differences clinical...
Abstract APC infection and dysfunction may contribute to the immunopathogenesis of HIV disease. In this study, we examined immunologic function highly enriched populations HIV-infected monocyte-derived dendritic cells (DC). Compared with uninfected DC, DC markedly down-regulated surface expression CD4. p24+ were then by negative selection CD4+HIV p24− assessed for cytokine secretion function. Although secreted increased IL-12p70 decreased IL-10, these poor stimulators allogeneic CD4+ T cell...
<i>Background:</i> It remains to be determined whether pemphigus vulgaris (PV), an autoimmune blistering disease, has a reduction and/or dysfunction of CD4<sup>+</sup>CD25<sup>high</sup> regulatory T (Treg) cells. <i>Objectives:</i> To evaluate the frequency and phenotypes Treg cells in blood patients with PV. <i>Methods:</i> Peripheral mononuclear were prepared from PV as well normal disease control volunteers, by flow cytometry....
Zinc deficiency causes myriad pathophysiological symptoms, but why distinct phenotypes are generated by zinc remains unclear. Considering that several ectoenzymes involved in purinergic signaling through extracellular adenine-nucleotide hydrolysis possess ions their active sites, and disorders result diverse diseases frequently similar to those caused deficiency, herein we examine whether affects metabolism. severely impairs the activities of major (ENPP1, ENPP3, NT5E/CD73, TNAP), also...