A5056567863- Atherosclerosis and Cardiovascular Diseases
- Liver Disease Diagnosis and Treatment
- Immune Cell Function and Interaction
- Endoplasmic Reticulum Stress and Disease
- RNA regulation and disease
- Immunodeficiency and Autoimmune Disorders
- Liver physiology and pathology
- RNA modifications and cancer
- RNA Research and Splicing
- Blood disorders and treatments
- MicroRNA in disease regulation
- Complement system in diseases
- Platelet Disorders and Treatments
- Inflammasome and immune disorders
- Pancreatic function and diabetes
- Transplantation: Methods and Outcomes
- Telomeres, Telomerase, and Senescence
- S100 Proteins and Annexins
- Diabetes and associated disorders
- Microbial Metabolism and Applications
- Moringa oleifera research and applications
- Lysosomal Storage Disorders Research
- Signaling Pathways in Disease
- Galectins and Cancer Biology
- Genomics, phytochemicals, and oxidative stress
Oslo University Hospital
2018-2025
University of Oslo
2014-2024
National Clinical Research
2022
Chinese Academy of Medical Sciences & Peking Union Medical College
2022
Weatherford College
2021
University of Bergen
2021
Norwegian University of Science and Technology
2012
Abstract Aims We recently reported five cases of vaccine-induced immune thrombotic thrombocytopenia (VITT) 7–10 days after receiving the first dose ChAdOx1 nCoV-19 adenoviral vector vaccine against corona virus disease 2019 (COVID-19). aimed to investigate pathogenic immunological responses operating in these patients. Methods and results assessed circulating inflammatory markers by assays cell phenotyping flow cytometry analyses performed immunoprecipitation with anti-platelet factor (PF)4...
Background Complement activation may promote atherosclerosis. Yet, data on the to which extent complement, and more specifically alternative complement pathway, is activated in patients with carotid atherosclerosis related adverse outcome these patients, are scarce. Methods Results We measured, by ELISA, plasma levels of factor D, properdin, C3bBbP (C3 convertase), H advanced a Discovery (n=324) Validation (n=206) cohort relation (mean follow‐up 7.8 6.6 years, respectively). Our major...
BackgroundDuring atherogenesis, cholesterol precipitates into crystals (CC) in the vessel wall, which trigger plaque inflammation by activating NACHT, LRR and PYD domains-containing protein 3 (NLRP3) inflammasome. We investigated relationship between CC, complement NLRP3 patients with cardiovascular disease.MethodsWe analysed plasma, peripheral blood mononuclear cells (PBMC) carotid plaques from advanced atherosclerosis applying ELISAs, multiplex cytokine assay, qPCR, immunohistochemistry,...
Soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) has been shown to be increased in patients with acute ischemic stroke. Here, we evaluated plasma sLOX-1 levels and vascular carotid plaque LOX-1 (ie, OLR1) gene expression stroke transient attack (TIA) particular focus on their relation time since symptom onset.Plasma (n=232) OLR1 (n=146) were who referred evaluation for endarterectomy, as well healthy control (n=81). Patients categorized according presence of or (n=35)...
More than 170 post-transcriptional RNA modifications regulate the localization, processing and function of cellular RNAs, aberrant have been linked to a range human diseases. The modification landscape in atherosclerosis, main underlying cause cardiovascular diseases, is still largely unknown.We used mass spectrometry analyse selection RNA-modifying enzymes N6-methyladenosine (m6A) carotid atherosclerotic lesion samples representing early advanced stages atherosclerosis as compared...
Common variable immunodeficiency (CVID) is the most common symptomatic primary immunodeficiency, characterized by inadequate antibody responses and recurrent bacterial infections. Paradoxically, a majority of CVID patients have non-infectious inflammatory autoimmune complications, associated with systemic immune activation. Our aim was to explore if HDL, known anti-inflammatory properties, had impaired function in thereby contributed their phenotype. We found reduced HDL cholesterol levels...
Acute myocardial infarction (MI) results in tissue damage to affected areas of the myocardium. The initial inflammatory response is most damaging for residual cardiac function, while at later stages inflammation a prerequisite proper healing and scar formation. Balancing extent duration during various after MI thus pivotal preserving function. Recently, signaling lymphocytic activation molecule 1 (SLAMF1)-derived peptide (P7) was shown reduce secretion cytokines protected against acute...
Abstract Two recent studies suggest that experimental pain sensitivity is associated with low-grade systemic inflammation. However, only 2 biomarkers have been identified, and the were conducted in adult individuals where confounding effects of comorbid diseases cannot be excluded. We therefore tested associations between 119 inflammation-related serum 827 healthy adolescents (15-19 years) population-based Tromsø Study: Fit Futures . The main outcome measure was cold-pressor tolerance (CPT),...
Background and aimsWe have previously found increased levels of the cysteine protease legumain in plasma plaques from patients with carotid atherosclerosis. This study further investigated during acute cardiovascular events.MethodsCirculating released platelets were assessed by enzyme-linked-immunosorbent assay. Quantitative PCR immunoblotting used to expression, while localization was visualized immunohistochemistry.ResultsIn SUMMIT Malmö cohort (n = 339 or without type 2 diabetes and/or...
Abstract Human kidney predominant protein, NCU-G1, is a highly conserved protein with an unknown biological function. Initially described as nuclear it has later been shown to be bona fide lysosomal integral membrane protein. To gain insight into the physiological function of mice no detectable expression this gene were created using gene-trap strategy, and Ncu-g1gt/gt successfully characterized. Lysosomal disorders are mainly caused by lack or malfunctioning proteins in endosomal-lysosomal...
Atherogenesis involves a complex interaction between immune cells and lipids, processes greatly influenced by the vascular smooth muscle cell (VSMC) phenotype. The DNA glycosylase NEIL3 has previously been shown to have role in atherogenesis, though whether this is due its ability repair damage or other non-canonical functions not yet clear. Hereby, we investigate of specifically VSMC phenotypic modulation, which critical plaque formation stability.Chow diet-fed atherosclerosis-prone Apoe-/-...
<b><i>Background and Purpose:</i></b> Cardioembolic stroke due to paroxysmal atrial fibrillation (AF) may account for 1 out of 4 cryptogenic strokes (CS) transient ischemic attacks (TIAs). The purpose this pilot study was search biomarkers potentially predicting incident AF in patients with or TIA. <b><i>Methods:</i></b> Plasma samples were collected from aged 18 years older TIA (<i>n</i> = 9) large artery atherosclerosis (LAA)...
Background In cardiovascular diseases, atherosclerotic disorder are the most frequent and important with respect to morbidity mortality. Inflammation mediated by immune cells is central in all parts of progress, further understanding underlying mechanisms needed. Growing evidence suggests that deamination adenosine‐to‐inosine RNA crucial for a correct response; nevertheless, role editing atherogenesis has barely been studied. Several proteins have affinity inosines RNA, one being ENDOV...
Abstract Purpose Fatty acid (FA) abnormalities are found in various inflammatory disorders and have been related to disturbed gut microbiota. Patients with common variable immunodeficiency (CVID) complications associated altered microbial composition. We hypothesized that there is an FA profile CVID patients, dysbiosis. Methods Plasma FAs were measured 39 patients 30 healthy controls. Gut profile, a food frequency questionnaire, the effect of oral antibiotic rifaximin investigated patients....
Endonuclease V (EndoV) is a conserved inosine-specific ribonuclease with unknown biological function. Here, we present the first mouse model lacking EndoV, which viable without visible abnormalities. We show that endogenous murine EndoV cleaves inosine-containing RNA in vitro, nevertheless series of experiments fails to link an vivo function processing such transcripts. As inosine levels and adenosine-to-inosine editing often are dysregulated hepatocellular carcinoma (HCC), chemically...
Mice lacking glycosylated lysosomal membrane protein (Glmp gt/gt mice) have liver fibrosis as the predominant phenotype due to chronic injury. The Glmp mice grow and reproduce at same rate their wild-type siblings. Life expectancy is around 18 months. Wild-type were studied between 1 week months of age. Livers analyzed using histological, immunohistochemical, biochemical, qPCR analyses. It was shown that not born with injury; however, it appeared shortly after birth indicated by excess...
Multifactorial metabolic diseases, such as non-alcoholic fatty liver disease, are a major burden to modern societies, and frequently present with no clearly defined molecular biomarkers. Herein we used system medicine approaches decipher signatures of fibrosis in mouse models malfunction genes from unrelated biological pathways: cholesterol synthesis—Cyp51, notch signaling—Rbpj, nuclear factor kappa-light-chain-enhancer activated B cells (NF-κB) signaling—Ikbkg, unknown lysosomal...
Cardiac allograft vasculopathy (CAV) causes heart failure after transplantation (HTx), but its pathogenesis is incompletely understood. Notch signaling, possibly modulated by everolimus (EVR), essential for processes involved in CAV. We hypothesized that circulating ligands would be dysregulated HTx. studied delta-like ligand 1 (DLL1) and periostin (POSTN) CAV de novo HTx recipients (n = 70) randomized to standard or EVR-based, calcineurin inhibitor-free immunosuppression maintenance 41)....
Ablation of glycosylated lysosomal membrane protein (GLMP, formerly known as NCU-G1) has been shown to cause chronic liver injury which progresses into fibrosis in mice. Both dysfunction and can metabolic dysregulation. Glmp gt/gt mice (formerly Ncu-g1gt/gt mice) were studied between 3 weeks 9 months age. Body weight gain feed efficiency comparable wild type siblings, only at the age siblings had significantly reduced body weight. Reduced size epididymal fat pads was accompanied by...
Obesity is a complex multicausal disease that can cause morbidity and mortality, there need for improved knowledge on the underlying mechanisms. Using mouse model of increased T cell responsiveness, we show development obesity be driven by immune cells. This was confirmed with bone marrow transplantation adoptive transfer to several recipient models. Single-cell RNA sequencing CyTOF analysis showed mice display altered composition circulating cells activation in visceral adipose tissue,...
Abstract Inflammation through activation of caspase‐1, seems to play a role in pulmonary hypertension induced by alveolar hypoxia. Whether hypoxia induces caspase‐1‐mediated inflammation and influx leukocytes other organs than the lungs, is not known. Our aim was explore sites caspase‐1‐related Wild type (WT) mice were exposed environmental or room‐air, analyzed. Right heart catheterization performed after 14 days WT transplanted with caspase‐1 −/− bone marrow. Hypoxia leukocyte accumulation...
Circular (circ) RNAs are non-coding with important functions in the nervous system, cardiovascular and cancer. Their role atherosclerosis myocardial infarction (MI) remains poorly described. We aim to investigate potential circRNAs immune cells during atherogenesis examine most regulated MI modulation by interleukin (IL)-6 receptor inhibition tocilizumab. Wild-type (WT)