A5070925361- Reproductive Biology and Fertility
- Mitochondrial Function and Pathology
- Pluripotent Stem Cells Research
- Birth, Development, and Health
- Genetics, Aging, and Longevity in Model Organisms
- Organ Transplantation Techniques and Outcomes
- Endoplasmic Reticulum Stress and Disease
- Effects and risks of endocrine disrupting chemicals
- Animal Genetics and Reproduction
- Glycosylation and Glycoproteins Research
- Sperm and Testicular Function
- Agriculture, Soil, Plant Science
- Lipid metabolism and disorders
- Renal and related cancers
- Genomics, phytochemicals, and oxidative stress
- CRISPR and Genetic Engineering
- ATP Synthase and ATPases Research
- Selenium in Biological Systems
- Molecular Biology Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- Viral Infectious Diseases and Gene Expression in Insects
- Autophagy in Disease and Therapy
- Plant Genetic and Mutation Studies
- Mycotoxins in Agriculture and Food
- Plant pathogens and resistance mechanisms
Daegu University
2016-2025
Kyungnam University
2014
Abstract Under endoplasmic reticulum ( ER )‐stress conditions, the unfolded protein response UPR ) generates a defense mechanism in mammalian cells. The regulation of signaling is important oocyte maturation, embryo development, and female reproduction pigs. Recent studies have shown that melatonin plays an role as antioxidant to improve pig maturation. However, there no report on ‐stress during vitro maturation IVM porcine oocytes. Therefore, objective this study was investigate...
Mito-TEMPO is a well-known mitochondria-specific superoxide scavenger. However, the effect of on porcine embryo development, to our knowledge, has not been studied yet. In present study, embryos were classified into two groups (G1 and G2) based cytoplasm lipid contents at zygote stage. The development blastocysts derived from G2 zygotes was reduced (G2:16.2 ± 7.9% vs G1: 26.5 5.9%; 1.6-fold, p < 0.05) compared those G1 zygotes. embryos, proportion TUNEL-positive cells also higher than that...
Bisphenol A (BPA) is synthetic organic compound that exhibits estrogen-like properties and it induces mitochondrial superoxide production. Melatonin (Mela) protects against BPA-mediated cell damage apoptosis. However, the antioxidative effects of Mela BPA-induced production in porcine oocytes are still not known. In this study, we investigated BPA-derived on oocyte maturation pigs. To investigate specific scavenger, Mito-TEMPO, response to BPA exposure apoptosis proteins, treated with...
Post-ovulatory aging (POA) is associated with reduced fertilization rates and poor embryo quality both in vivo vitro. However, the relationship between nicotinamide adenine dinucleotide (NAD+) filamentous actin (F-actin) cytoskeleton POA-induced oocytes remains unknown. Here, we investigated mechanisms by which NAD+ salvage pathways function oocyte maturation upon POA through F-actin cytoskeleton. Porcine were aged extending vitro (IVM) for an additional 24 h to create a model. adducin 1...
Gangliosides are components of the mammalian plasma membrane that help regulate receptor signaling. Ganglioside GM3, for example, plays an important role in initiating apoptosis cancer cells; however, physiological roles GM3 normal processes, such as during pig oocyte maturation, not clear. The aim this study was to investigate functional link between and cellular porcine cumulus-oocyte-complexes (COCs) vitro maturation. Our results indicated denuded oocytes possess less ST3GAL5, a...
While triclosan (TCS) exerts detrimental effects on female reproduction, the effect of TCS-derived toxins porcine oocytes during in vitro maturation (IVM) is unclear. This study investigated TCS mitochondrion-derived reactive oxygen species (ROS) production and apoptosis pathways oocyte maturation. Porcine were treated with (1, 10, 100 μM) triphenylphosphonium chloride (Mito-TEMPO; 0.1 μM), matured cumulus complexes (COCs) stained orcein, dichlorofluorescein diacetate (DCF-DA), Mito-SOX....
Abstract Rapamycin induces autophagosome formation and activity during oocyte maturation, improved fertilization ability of matured oocytes, early embryonic developmental competence. However, potential changes in mitochondrial fission mitophagy via regulation autophagy porcine development have not been previously studied. Here, we investigated quality embryos 2 days after vitro following treatment with 1 10 nM rapamycin. As a results, rapamycin exposure significantly ( p < 0.05)...
Changes in F-actin distribution and cortical morphology are important for blastocyst developmental competence during embryogenesis. However, the effect of paclitaxel as a microtubule stabilizer on embryonic development pigs remains unclear. We investigated role cytoskeleton stabilization via P38 MAPK activation using to improve potential blastocysts pigs. In this study, enrichment adducin expression based blastomere fragment rate cytokinesis defects were cleaved embryos after vitro...
In the present study, we investigated role of binding immunoglobulin protein/glucose-regulated protein, 78-kDa (BIP/GRP78)-regulated endoplasmic reticulum (ER)-stress on meiotic maturation and cumulus cells expansion in porcine cumulus-oocyte complexes (COCs). Previously, it has been demonstrated that unfolded protein response (UPR)-related genes, such as molecules involved ER-stress defense mechanisms, were expressed matured oocytes during vitro (IVM) oocytes. However, BIP/GRP78-mediated...
Mitochondrial division inhibitor 1 (Mdivi-1) reportedly provides a close connection between oocyte maturation and mitochondrial function in pigs. N -acetyl-5-methoxy-tryptamine (melatonin) is known to be representative antioxidant with the ability rehabilitate meiotic of porcine oocytes. However, melatonin recover Mdivi-1-mediated disruption spindle formation during oocytes vitro (IVM) has not been studied. Here, we first investigated changes length, such as fragmentation elongation form,...
Objective Phellodendron amurense (P. amurense) and Humulus japonicus (H. japonicus) are closely involved in anti-oxidative response increasing antioxidant enzymes activities. However, the effects of their extracts on development preimplantation bovine embryos have not been investigated. Therefore, we investigated P. H. developmental competence quality embryos. Methods After vitro fertilization, were cultured for 7 days Charles Rosenkrans amino acid medium supplemented with (0.01 μg/mL)...
Seul-Gi Yang, Hyo-Jin Park, Sang-Min Lee, Jin-Woo Kim, Min-Ji In-Su Ho-Geun Jegal and Deog-Bon Koo. Journal of Animal Reproduction Biotechnology 2019;34:10-9. https://doi.org/10.12750/JARB.34.1.10
Ruthenium red (RR) inhibits calcium (Ca2+) entry from the cytoplasm to mitochondria, and is involved in maintenance of Ca2+ homeostasis mammalian cells. very important for further embryonic development fertilized oocytes. However, effect RR on mitochondria-Ca2+ (mito-Ca2+) levels during vitro fertilization (IVF) subsequent blastocyst developmental capacity porcine unclear. The present study explored regulation mito-Ca2+ using and/or histamine oocytes their influence pigs. Red fluorescence...
Unfolding protein response (UPR) is a defence mechanism during endoplasmic reticulum (ER) stress in mammalian cells. Especially, UPR genes and regulation of reactive oxygen species involved ER on porcine oocyte maturation vitro. Some studies have shown that melatonin treatment results reducing oxidative stress, protective function free radical damage embryo development. Also, has an important role stress. However, it unknown how the changes expression levels are affected maturation. In...
Jin-Woo Kim, Seul-Gi Yang, Hyo-Jin Park, Ju Hwan Dong-Mok Lee, Seong-Min Woo, Hyun-Jeong Hyun Ah Jae-Hoon Jeong, Min Ji Lee and Deog-Bon Koo. Journal of Animal Reproduction Biotechnology 2020;35:207-13. https://doi.org/10.12750/JARB.35.2.207
Geon-Yeop Do, Jin-Woo Kim, Sung-Kyu Chae, Jae-Hyun Ahn, Hyo-Jin Park, Jae-Young Seul-Gi Yang, and Deog-Bon Koo. Journal of Embryo Transfer 2015;30:289-98. https://doi.org/10.12750/JET.2015.30.4.289
Sung-Kyu Chae, Hyo-Jin Park, Jin-Woo Kim, Jae-Hyun Ahn, Soo-Yong Jae-Young Seul-Gi Yang, and Deog-Bon Koo. Journal of Embryo Transfer 2015;30:319-25. https://doi.org/10.12750/JET.2015.30.4.319
Ochratoxin A (OTA), a mycotoxin found in foods, has deleterious effect on female reproduction owing to its endocrine-disrupting activity mediated through endoplasmic reticulum (ER) stress and reactive oxygen species (ROS) production. However, the mechanisms of OTA-induced ER pig embryos during vitro culture (IVC) are not yet fully understood. In present study, porcine were cultured for two days an IVC medium supplemented with 0.5, 1.0, 5.0 μM OTA, which led reduction developmental rate...
Background: Post-ovulatory aging (POA) of oocytes is related to a decrease in the quality and quantity caused by aging.Previous studies on characteristics POA have investigated injury early embryonic developmental ability, but no information available its effects mitochondrial fission mitophagy-related responses.In this study, we aimed elucidate molecular mechanisms underlying mitophagy vitro maturation (IVM) model based RNA sequencing analysis.Methods: The was obtained through an additional...