A5100613088- Reproductive Biology and Fertility
- Endoplasmic Reticulum Stress and Disease
- Parkinson's Disease Mechanisms and Treatments
- Mitochondrial Function and Pathology
- Alzheimer's disease research and treatments
- Genetics, Aging, and Longevity in Model Organisms
- Genomics, phytochemicals, and oxidative stress
- Birth, Development, and Health
- Pluripotent Stem Cells Research
- Selenium in Biological Systems
- Electron Spin Resonance Studies
- Glycosylation and Glycoproteins Research
- Effects and risks of endocrine disrupting chemicals
- Redox biology and oxidative stress
- Organ Transplantation Techniques and Outcomes
- Reproductive Physiology in Livestock
- Heat shock proteins research
- Animal Genetics and Reproduction
- Cholinesterase and Neurodegenerative Diseases
- Fluoride Effects and Removal
- Plant pathogens and resistance mechanisms
- Agriculture, Soil, Plant Science
- Tissue Engineering and Regenerative Medicine
- ATP Synthase and ATPases Research
- Nuclear Receptors and Signaling
Daegu University
2016-2025
Seoul National University Bundang Hospital
2025
Yale University
2021-2024
Cardiovascular Research Center
2021
Ajou University
2020
University of Florida
2012-2020
Whitney Museum of American Art
2017
Kyungpook National University
2012-2013
Korea University
2002-2012
Catholic University of Korea
2005
Abstract Under endoplasmic reticulum ( ER )‐stress conditions, the unfolded protein response UPR ) generates a defense mechanism in mammalian cells. The regulation of signaling is important oocyte maturation, embryo development, and female reproduction pigs. Recent studies have shown that melatonin plays an role as antioxidant to improve pig maturation. However, there no report on ‐stress during vitro maturation IVM porcine oocytes. Therefore, objective this study was investigate...
Mito-TEMPO is a well-known mitochondria-specific superoxide scavenger. However, the effect of on porcine embryo development, to our knowledge, has not been studied yet. In present study, embryos were classified into two groups (G1 and G2) based cytoplasm lipid contents at zygote stage. The development blastocysts derived from G2 zygotes was reduced (G2:16.2 ± 7.9% vs G1: 26.5 5.9%; 1.6-fold, p < 0.05) compared those G1 zygotes. embryos, proportion TUNEL-positive cells also higher than that...
Bisphenol A (BPA) is synthetic organic compound that exhibits estrogen-like properties and it induces mitochondrial superoxide production. Melatonin (Mela) protects against BPA-mediated cell damage apoptosis. However, the antioxidative effects of Mela BPA-induced production in porcine oocytes are still not known. In this study, we investigated BPA-derived on oocyte maturation pigs. To investigate specific scavenger, Mito-TEMPO, response to BPA exposure apoptosis proteins, treated with...
Cisplatin is one of the most widely used chemotherapeutic drugs for treatment cancer. Unfortunately, its major side effects permanent hearing loss. Here, we show that glutathione transferase α4 (GSTA4), a member Phase II detoxifying enzyme superfamily, mediates reduction cisplatin ototoxicity by removing 4-hydroxynonenal (4-HNE) in inner ears female mice. Under treatment, loss Gsta4 results more profound mice compared to male stimulates GSTA4 activity ear wild-type, but not wild-type In...
Abstract Isocitrate dehydrogenase (IDH) 2 participates in the TCA cycle and catalyzes conversion of isocitrate to α-ketoglutarate NADP + NADPH. In mitochondria, IDH2 also plays a key role protecting mitochondrial components from oxidative stress by supplying NADPH both glutathione reductase (GSR) thioredoxin (TXNRD2). Here, we report that loss Idh2 accelerates age-related hearing loss, most common form impairment, male mice. This was accompanied increased DNA damage, apoptotic cell death,...
Post-ovulatory aging (POA) is associated with reduced fertilization rates and poor embryo quality both in vivo vitro. However, the relationship between nicotinamide adenine dinucleotide (NAD+) filamentous actin (F-actin) cytoskeleton POA-induced oocytes remains unknown. Here, we investigated mechanisms by which NAD+ salvage pathways function oocyte maturation upon POA through F-actin cytoskeleton. Porcine were aged extending vitro (IVM) for an additional 24 h to create a model. adducin 1...
Abstract Introduction Chronotype is commonly assessed using questionnaires such as Munich Questionnaire (MCTQ). However, while the MCTQ accounts for sleep debt, it may not fully reflect individual’s inherent chronobiological preferences due to external influences. Pristine internal time, defined time free from influences work and social obligations, offers an alternative perspective on preferences. This study aimed characterize pristine evaluate its discrepancy actual behavior within Korean...
Aβ production is influenced by intracellular trafficking of secretases and amyloid precursor protein (APP).
Gangliosides are components of the mammalian plasma membrane that help regulate receptor signaling. Ganglioside GM3, for example, plays an important role in initiating apoptosis cancer cells; however, physiological roles GM3 normal processes, such as during pig oocyte maturation, not clear. The aim this study was to investigate functional link between and cellular porcine cumulus-oocyte-complexes (COCs) vitro maturation. Our results indicated denuded oocytes possess less ST3GAL5, a...
Glutathione reductase (GSR), a key member of the glutathione antioxidant defense system, converts oxidized (GSSG) to reduced (GSH) and maintains intracellular redox state protect cells from oxidative damage. Previous reports have shown that Gsr deficiency results in defects host against bacterial infection, while diquat induces renal injury hypomorphic mice. In flies, overexpression GSR extended lifespan under hyperoxia. current study, we investigated roles cochlear using homozygous knockout...
While triclosan (TCS) exerts detrimental effects on female reproduction, the effect of TCS-derived toxins porcine oocytes during in vitro maturation (IVM) is unclear. This study investigated TCS mitochondrion-derived reactive oxygen species (ROS) production and apoptosis pathways oocyte maturation. Porcine were treated with (1, 10, 100 μM) triphenylphosphonium chloride (Mito-TEMPO; 0.1 μM), matured cumulus complexes (COCs) stained orcein, dichlorofluorescein diacetate (DCF-DA), Mito-SOX....
Abnormal accumulation of α-synuclein (αSyn) has been linked to endoplasmic-reticulum (ER) stress, defective intracellular protein/vesicle trafficking, and cytotoxicity. Targeting factors involved in ER-related protein processing trafficking may, therefore, be a key modulating αSyn levels associated toxicity. Recently retention endoplasmic reticulum 1 (RER1) identified as an important ER retrieval/retention factor for Alzheimer's disease proteins negatively regulates amyloid-β peptide levels....
Triclosan (TCS) is included in various healthcare products because of its antimicrobial activity; therefore, many humans are exposed to TCS daily. While detrimental effects exposure have been reported species and cell types, the on early embryonic development largely unknown. The aim this study was determine if exerts toxic during using porcine parthenogenetic embryos vitro. Porcine were cultured vitro culture medium with 50 or 100 µM for 6 days. Developmental parameters including cleavage...
The integrity of the blood–retina barrier (BRB) is crucial for phototransduction and vision, by tightly restricting transport molecules between blood surrounding neuronal cells. Breakdown BRB leads to development retinal diseases. Here, we show that Netrin-1/Unc5b Norrin/Lrp5 signaling establish a zonated endothelial cell gene expression program controls integrity. Using single-cell RNA sequencing (scRNA-seq) postnatal BRB-competent mouse retina cells (ECs), identify >100 genes encoding...
Peroxiredoxins (PRDXs) are expressed in the ovary and during ovulation. PRDX1 activity related to immuno-like response ovulation is unknown. We investigated roles of Prdx1 on TLR4 ERK1/2 signaling from ovulated cumulus-oocyte complex (COC) using Prdx1-knockout (K/O) wild-type (WT) mice. Ovulated COCs were collected 12 16 h after pregnant mare serum gonadotropin/hCG injection. protein expression COC secretion factors (Il-6, Tnfaip6, Ptgs2) increased WT mice obtained. treated with LPS (0.5...
Changes in F-actin distribution and cortical morphology are important for blastocyst developmental competence during embryogenesis. However, the effect of paclitaxel as a microtubule stabilizer on embryonic development pigs remains unclear. We investigated role cytoskeleton stabilization via P38 MAPK activation using to improve potential blastocysts pigs. In this study, enrichment adducin expression based blastomere fragment rate cytokinesis defects were cleaved embryos after vitro...
In the present study, we investigated role of binding immunoglobulin protein/glucose-regulated protein, 78-kDa (BIP/GRP78)-regulated endoplasmic reticulum (ER)-stress on meiotic maturation and cumulus cells expansion in porcine cumulus-oocyte complexes (COCs). Previously, it has been demonstrated that unfolded protein response (UPR)-related genes, such as molecules involved ER-stress defense mechanisms, were expressed matured oocytes during vitro (IVM) oocytes. However, BIP/GRP78-mediated...
Mitochondrial division inhibitor 1 (Mdivi-1) reportedly provides a close connection between oocyte maturation and mitochondrial function in pigs. N -acetyl-5-methoxy-tryptamine (melatonin) is known to be representative antioxidant with the ability rehabilitate meiotic of porcine oocytes. However, melatonin recover Mdivi-1-mediated disruption spindle formation during oocytes vitro (IVM) has not been studied. Here, we first investigated changes length, such as fragmentation elongation form,...