A5101634732- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Protein Structure and Dynamics
- Advanced biosensing and bioanalysis techniques
- Signaling Pathways in Disease
- Nanopore and Nanochannel Transport Studies
- Enzyme Structure and Function
- RNA Research and Splicing
- Ion-surface interactions and analysis
- RNA Interference and Gene Delivery
- Antimicrobial Peptides and Activities
- DNA and Nucleic Acid Chemistry
- Bacteriophages and microbial interactions
- Peptidase Inhibition and Analysis
- Nerve injury and regeneration
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Biosensing Techniques and Applications
- Advanced Nanomaterials in Catalysis
- Autophagy in Disease and Therapy
- Microbial Inactivation Methods
- Graphene research and applications
- Machine Learning in Materials Science
- Copper Interconnects and Reliability
Chengdu University of Traditional Chinese Medicine
2025
Sichuan University
2025
Tongji Hospital
2023-2024
Huazhong University of Science and Technology
2023-2024
Xuzhou Medical College
2024
ShanghaiTech University
2022-2023
Northeastern University
2016-2020
Beijing Normal University
2016-2018
Dana (United States)
2017
Institute of Optics and Electronics, Chinese Academy of Sciences
2010-2014
Abstract: Squamous cell carcinoma antigen (SCCa), as a tumor biomarker, plays an important role in adjuvant diagnosis, treatment evaluation, and prognosis prediction for cervical cancer patients. Localized surface plasmon resonance (LSPR) technique based on noble metal nanoparticles bypasses the disadvantages of traditional testing strategies, terms free-labeling, short assay time, good sensitivity, selectivity. Herein, we develop novel reusable LSPR biosensor detection SCCa. First,...
To develop a multiclass non-clinical screening tool for periodontal disease and assess its accuracy differentiating health, gingivitis different stages of periodontitis.A cross-sectional diagnostic study on convenience sample 408 consecutive subjects was conducted by applying three index tests estimating features the health-disease spectrum: self-administered questionnaire, an oral rinse activated matrix metalloproteinase-8 (aMMP-8) point-of-care test (POCT) determination gingival bleeding...
In recent years, there has been a growing interest to quantify the energy landscape that governs ribosome dynamics. However, in order quantitatively integrate theoretical predictions and experimental measurements, it is essential one detailed understanding of associated diffusive properties. Here, all-atom explicit-solvent simulations (50 μs aggregate sampling) predict diffusion coefficient tRNA molecule will depend on its position within ribosome. Specifically, during aa-tRNA accommodation...
Addressing the global challenge of bacterial resistance demands innovative approaches, among which multitargeting is a widely used strategy. Current strategies multitargeting, typically achieved through drug combinations or single agents inherently aiming at multiple targets, face challenges such as stringent pharmacokinetic and pharmacodynamic requirements cytotoxicity concerns. In this report, we propose bacterial-specific disruption approach vastly expanded strategy that effectively...
Prostate-specific antigen (PSA) is the common serum-relevant biomarker for early prostate cancer (PCa) detection in clinical diagnosis. However, it difficult to accurately diagnose PCa stage due low specificity of PSA. Herein, a new solution-gated graphene field transistor (SGGT) biosensor with dual-gate dual-biomarker designed. The sensing mechanism that designed aptamers immobilized on surface gate electrodes can capture PSA and sarcosine (SAR) biomolecules induce capacitance changes...
In order to explore the clinical application of nanobiosensor based on localized surface plasmon resonance (LSPR), we used our LSPR biosensor detect microalbuminuria in this work. The sliver nanoparticles were fabricated by using nanosphere lithography. anti-human albumin antibody was immobilized sensor amine coupling method. different concentrations commercial and urine samples from three mild preeclampsia patients determined according peak extinction spectra. Under optimum conditions,...
Protein folding occurs in a high dimensional phase space, and the representation of associated energy landscape is nontrivial. A widely applied approach to studying landscapes describe dynamics along small number reaction coordinates. However, other strategies involve more elaborate analysis complex space. There have been many attempts obtain detailed all available conformations for given system. In this work, we address problem using metric based on internal distances between amino acids...
The ribosome is a large ribonucleoprotein complex that responsible for the production of proteins in all organisms. Accommodation process by which an incoming aminoacyl-tRNA (aa-tRNA) molecule binds ribosomal A site, and its kinetics has been implicated accuracy tRNA selection. In addition to rearrangements aa-tRNA molecule, L11 stalk can undergo large-scale anisotropic motions during translation. To explore potential impact this protruding region on rate accommodation, we used molecular...
Proteins are synthesized by the joint action of ribosome and tRNA molecules, where rate synthesis can be affected numerous factors, such as concentration tRNA, binding affinity for ribosome, or post-transcriptional modifications. Here, we expand this range contributors demonstrating how differences in structure give rise to species-specific dynamics ribosome. To show this, perform simulations A/P hybrid-state formation two species (tRNAPhe tRNALeu), which differ size their variable loops...
Abstract Elongation factor Tu (EF‐Tu) is a three‐domain protein that responsible for delivering aminoacyl‐tRNA (aa‐tRNA) molecules to the ribosome. During delivery process, EF‐Tu undergoes large‐scale ( ~50 Å) conformational transition results in rearrangement of domain I, relative II/III superdomain. Despite central role during synthesis, little known about structural and energetic properties this reordering process. To study physical‐chemical motion, we constructed multi‐basin...
The traditional approach of computational biology consists calculating molecule properties by using approximate classical potentials. Interactions between atoms are described an energy function derived from physical principles or fitted to experimental data. Their functional form is usually limited pairwise interactions and does not consider complex multibody effects. More recently, neural networks have emerged as alternative way describing the biomolecules. In this approach, explicit...
Few studies to date have reported on the development and application of a nanobiosensor based localized surface plasmon resonance (LSPR) for detecting gene mutations.This study aimed develop novel LSPR biosensor used p53 mutation.Nanosphere lithography was fabricate silver nanoparticles.The DNA probe designed recognize target sequence immobilized chip by covalent-coupling method using amine-group ligands.Synthetic oligonucleotides or PCR products were amplified from genomic taken blood...
The calcineurin B subunit (CNB) has antitumor activity. We showed previously that recombinant human CNB (rhCNB) also had strong anti-tumor activity in vivo, and was thus a promising candidate drug. It appeared to kill tumor cells via immunomodulation. Here, we show rhCNB inhibits the proliferation of hepatoma HepG-2 cells, resulting their apoptosis. Exogenous found localize mitochondria activate mitochondrial apoptosis pathway, as indicated by decrease transmembrane potential, release...
Summary Over the last 20 years, application of structure-based (Gō-like) models has ranged from protein folding with coarse-grained to all-atom representations large-scale molecular assemblies. While there are many variants that may be employed, common feature these is some (or all) stabilizing energetic interactions defined based on knowledge a particular experimentally-obtained conformation. With generality this approach, was need for versatile computational platform designing and...
The aim of this study was to establish a quality-control method for calcineurin subunit B (CNB) biological activity determinations. CNB enhances the p-nitrophenylphosphate (pNPP) dephosphorylating A Δ316 mutant (CNAΔ316). series concentrations were fitted four-parameter equation calculate corresponding pNPP maximum dephosphorylation rates. Values calculated based on references using parallel line method. then validated accuracy, precision, linearity, linear range, sensitivity, specificity,...