A5112569555- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Bacterial Identification and Susceptibility Testing
- Antibiotics Pharmacokinetics and Efficacy
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Pneumonia and Respiratory Infections
- Neutropenia and Cancer Infections
- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Cystic Fibrosis Research Advances
- Infectious Diseases and Mycology
- Pneumocystis jirovecii pneumonia detection and treatment
- Urinary Tract Infections Management
- Bacterial Infections and Vaccines
- Listeria monocytogenes in Food Safety
- Clostridium difficile and Clostridium perfringens research
- Viral gastroenteritis research and epidemiology
- Bacterial biofilms and quorum sensing
- Nosocomial Infections in ICU
- Enterobacteriaceae and Cronobacter Research
- Sepsis Diagnosis and Treatment
- Pediatric Urology and Nephrology Studies
- Microscopic Colitis
- Dental Trauma and Treatments
Istituto Giannina Gaslini
2015-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2014-2025
Azienda Mobilità e Trasporti
2021
University of Perugia
2020
Regione Liguria
2020
Czech Academy of Sciences, Institute of Microbiology
2013
United States Nuclear Regulatory Commission
2012
Boston Children's Hospital
2011-2012
Tecnologie Avanzate (Italy)
2011
Fairfax Neonatal Associates
2010
Background. The purpose of our study was to evaluate the incidence and clinical characteristics febrile episodes during neutropenia following chemotherapy in children with cancer.
Gram-negative bacteremia (GNB) is a major cause of illness and death after hematopoietic stem cell transplantation (HSCT), updated epidemiological investigation advisable.We prospectively evaluated the epidemiology pre-engraftment GNB in 1118 allogeneic HSCTs (allo-HSCTs) 1625 autologous (auto-HSCTs) among 54 transplant centers during 2014 (SIGNB-GITMO-AMCLI study). Using logistic regression methods. we identified risk factors for impact on 4-month overall-survival transplant.The cumulative...
SCN(-) (thiocyanate) is an important physiological anion involved in innate defense of mucosal surfaces. oxidized by H(2)O(2), a reaction catalyzed lactoperoxidase, to produce OSCN(-) (hypothiocyanite), molecule with antimicrobial activity. Given the importance availability airway surface fluid, we studied transepithelial transport human bronchial epithelium. We found evidence for at least three mechanisms basolateral apical flux. cAMP and Ca(2+) regulatory pathways controlled through cystic...
The aims of the study were to analyze clinical and epidemiological characteristics treatments for patients who developed zygomycosis enrolled in italy during european Confederation medical mycology survey. This prospective multicenter was performed between 2004 2007 at 49 italian Departments. 60 cases enrolled: median age 59.5 years (range 1-87), with a prevalence males (70%). majority immunocompromised (42 cases, 70%), mainly hematological malignancies (37). Among non-immunocompromised (18...
In last years the diffusion of carbapenem resistance in Gram-negative bacteria (CR-GNB) is increasing worldwide, mainly due to expression carbapenemases. Cefiderocol has molecular characteristics that ideally confers activity against all CR-GNB, but resistant strains have already been identified. We describe cefiderocol susceptibility profile among multi-drug isolated from pediatric patients.
Therapeutic drug monitoring is a cornerstone of antibacterial therapy, especially in an era increasing resistance individualizing antimicrobial therapy. Liquid chromatography/tandem mass spectrometry (LC–MS/MS) assay was used for the simultaneous measurement piperacillin, tazobactam, meropenem, ceftazidime, and linezolid 50 μl plasma samples over wide range. The overall turnaround time 20 minutes. Intra-assay precision accuracy quality control ranged within 1·8–8·5 91·4–106·7%, respectively....
ABSTRACT The plasmatic levels of 1,3-β- d -glucan (BDG) were >523 pg/ml in 4 children, 2 low-birth-weight neonates and stem cell transplant recipients, with the following invasive fungal diseases (IFD) proven apart from this BDG test: 3 cases Candida parapsilosis candidemias 1 case disseminated aspergillosis. test may be useful for identification IFD pediatrics.
Little is known about epidemiology of carbapenemase-producing Enterobacteriaceae (CPE) in children. Aim this study was to describe CPE a tertiary care pediatric hospital Italy that admits patients coming from geographic areas with high diffusion CPE. Prospective evaluation the proportion and rates per 100,000 discharges (D) or hospitalization-days (HD) invasive infections due 2013 2017 colonizations 2014 2017. Disease-preventing strategies comprised patients’ screening at admission,...
Background: Urinary tract infections (UTIs) are among the most common bacterial in children, and antibiotic susceptibility youngest patients remains poorly understood. This study aimed to describe distribution of uropathogens their susceptibility, focusing on oral formulations. Methods: Data from first microbiological isolation, between January 2007 December 2023, at Istituto Gaslini, young infants (aged <6 months), were analyzed. Results: We isolated 2473 infants’ pathogen, with a median...
Quality and safety of a cell product, essential to guarantee the health patients, depends on many factors including an appropriate environmental monitoring manufacturing rooms. Nonetheless, maintenance controlled environment is requested minimize risk contamination. Thus, timely detection changes in microbiological trends important adopt promptly effective measures against resistant strains that, turn, may invalidate not only sanitization procedures but also product. We analyzed microbes...
Data on epidemiology of severe infectious complications, ie, bacteremia or invasive fungal disease (IFD), in children with acute graft-versus-host (aGVHD) after allogeneic hemopoietic stem cell transplantation (HSCT) are scarce. In a retrospective, single-center study, we analyzed the risk (hazard ratio [HR]) and rate (episodes/1000 patients days at risk) bacteremias IFD receiving HSCT, according to type donor (matched related [MRD] alternative [AD]) presence grade aGVHD. From 2000 2009, 198...
Yeast-like filamentous fungi, collected in Italy from 1985 to 2018, were submitted molecular identification and antifungal susceptibility testings. Clinical isolates identified as Magnusiomyces capitatus (28), M. clavatus (18), Geotrichum candidum (2). was prevalent among blood (18/24), other biological materials. The intrinsic echinocandin resistance confirmed. Both species had low minimum inhibitory concentrations (MICs) of itraconazole, posaconazole, voriconazole, while lower MIC...
Two serotype 19A (ST695) Streptococcus pneumoniae vaccine escape recombinant strains attributable to capsular switching events were detected by a laboratory surveillance system that is an integral part of vaccination program begun in Liguria, Italy, May 2003, Italian administrative region with long-lasting high coverage, unusual occurrence Europe. To our knowledge, this the first detection outside United States.
To describe the epidemiology of invasive Candida infection in a tertiary care paediatric hospital. Prospective single-centre survey on all strains isolated from normally sterile fluids and urines period 2005-2015 . A total 299 ICI were documented 262 patients. Urinary tract represented most frequent diagnosis (62%), followed by fungaemia (34%) peritonitis (4%). Fungaemia was children with cancer (59%) or low birth weight neonates (61%), while urinary infections more patients malformation....
Summary Plasma 1,3‐β‐D‐glucan ( BDG ) is indicated as a tool for early diagnosis of invasive fungal diseases IFD ). However, data on its diagnostic value are scarce in children. Therefore, definition test performance paediatrics needed. was evaluated children admitted to “Istituto Giannina Gaslini,” Genoa, Italy, who developed clinical conditions at risk . Results were analysed sensitivity, specificity, predictive values, likelihood ratios, accuracy, informedness and probability missing one...
In the last decades, several diagnostic and therapeutic strategies have been implemented for management of invasive fungal diseases (IFD) in patients with cancer or receiving allogeneic hemopoietic stem cell transplant. Few data are available on their impact mortality children.All IFD episodes diagnosed at tertiary care center during a 30-year period between 1983 2012 were analyzed 90-day risk factors. Diagnoses coded according to international (European Organization Research Treatment...
Background Therapeutic drug monitoring (TDM) of antibiotics in children is fundamental importance for effective patient management. The use dried blood spot (DBS) offers a number advantages over conventional collection. aim this study to develop and validate method measure piperacillin/tazobactam DBS. Results analysis was performed by using LC-MS/MS operating multiple reaction mode. has been validated applying EMA guidelines its suitability TDM evaluated samples from low birth weight...