Pyroptosis and inflammation are involved in the development of chronic obstructive pulmonary disease (COPD). However, cigarette smoke-mediated mechanism COPD remains unclear. In this study, we aimed to investigate role nucleotide-binding domain-like receptor protein-3 (NLRP3) inflammasome-mediated pyroptosis death human bronchial epithelial (HBE) cells after smoke extract (CSE) exposure.The protein level NLRP3 lung tissue was measured exposure vivo. vitro, HBE were treated with CSE....

A novel series of optically active 2,6-disubstituted alkylphenols with improved anesthetic profiles compared to widely used propofol were synthesized. The incorporation the cyclopropyl group not only increased steric effect but also introduced stereoselective effects over their properties. Compounds 1, 2, and 6 selected as potential candidates for further preclinical development including studies water-soluble prodrugs. Clinical candidate compound (Haisco HSK3486) a general are being...

Background: Autophagy plays an important role in lung adenocarcinoma (LUAD). In this study, we aimed to explore the autophagy-related gene (ARG) expression pattern and identify promising biomarkers improve prognosis of LUAD. Methods: The profiles clinical information LUAD patients were downloaded from Cancer Genome Atlas (TCGA), validation cohort was extracted Gene Expression Omnibus database. Human Database (HADb) used extract ARGs. data analyzed using limma package visualized ggplot2 as...

As one of the common malignancies that threaten human life, bladder cancer occurs frequently with a high mortality rate in world, due to its invasion, recurrence and drug resistance. Natural products from marine microorganisms are becoming hotspots discovery new candidate entities, especially area cancer. Brefeldin A (BFA) is natural Arf-GEFs inhibitor, but low aqueous solubility, strong toxicity, poor bioavailability, it urgent conduct structural optimization research. Herein, BFA pyridine...

Most of the sequenced wood-rotting edible mushroom produce fruiting body at relatively low temperatures. Little information has been known about high-temperature mushroom. Here, we performed de novo sequencing and assembly genome a Pleurotus giganteus from monokaryotic strain zhudugu2 using Illumina Pac-Bio CLR technologies. P. giganteus, also as Zhudugu in China, is well-known culinary that widely distributed cultivated Southeast Asia, South Asia. The consists 40.00 Mb 27 contigs with...

It is necessary to evaluate fluid responsiveness before resuscitation. We evaluated the value of inferior vena cava (IVC) area respiratory variation and IVC diameter ratio (IVC DR) for predicting in mechanically ventilated patients.A prospective observational study was performed intensive care unit between December 2017 March 2018. Mechanically patients were enrolled received ultrasound monitoring. distensibility index from subxiphoid (IVC-sx DDI), right midaxillary line (IVC-rm ADI), DR...

ShuFeng JieDu capsule (SFJDC), a traditional Chinese medicine, has been recommended for the treatment of COVID-19 infections.However, pharmacological mechanism SFJDC still remains vague to date.The active ingredients and their target genes were collected from TCMSP.COVID-19 is type Novel Coronavirus Pneumonia (NCP).NCP-related GeneCards database.The ingredients-targets network PPI networks constructed.The candidate screened by Venn diagram package enrichment analysis.The gene-pathway was...

Ferroptosis and immune infiltration are involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). We aim to identify ferroptosis-related hub genes analyze their association with COPD through bioinformatics methods.The mRNA microarray data GSE38974 were downloaded from Gene Expression Omnibus obtain differentially expressed (DEGs). The DEGs intersected (FRGs) FerrDb FRGs. GO KEGG enrichment protein-protein interaction (PPI) analyses FRGs conducted R software STRING...

Bladder cancer is a common malignancy known for its high recurrence rate and poor survival rate. New strategies are urgently needed to reduce improve prognosis. Arf1 BMX potential targets associated with the prognosis of bladder cancer. In this study, niacin ester derivatives brefeldin A were synthesized by introducing nicotinic acid moieties at 4-OH 7-OH positions. Notably, 4-monoester derivative, CHNQD-01228 (2), could significantly inhibit proliferation T24 cells (IC50 = 0.22 μM) in...

LINC01492 polymorphism has been implicated in the susceptibility of large artery atherosclerotic (LAA) stroke; however, a dearth systematic research exists regarding this association Asian population at present. This study enrolled Han Chinese patients with LAA stroke (n = 428) and age- sex-matched controls 434). We employed dominant, recessive, codominant genetic models to analyze distribution alleles genotypes for 14 tag single nucleotide polymorphisms (SNPs) LINC01492. Furthermore, we...

A new class of potent and highly selective SGLT2 inhibitors is disclosed. Compound 31 (HSK0935) demonstrated excellent hSGLT2 inhibition 1.3 nM a high hSGLT1/hSGLT2 selectivity 843-fold. It showed robust urinary glucose excretion in Sprague-Dawley (SD) rats affected more Rhesus monkeys. Finally, an efficient synthetic route has been developed featuring ring-closing cascade reaction to incorporate double ketal 1-methoxy-6,8-dioxabicyclo[3.2.1]octane ring system.

To prospectively compare single dose gadobenate dimeglumine with double gadopentetate for CE-MRA in patients peripheral arterial occlusive disease (PAOD) using an intra-individual crossover study design which all received both contrast agents otherwise identical examinations. Institutional review board and regulatory approval were granted provided written informed consent. Sixty-eight (53M/15F; 62.4 ± 15.7 years) mild-to-severe PAOD enrolled randomized 3-station 0.1 mmol/kg 0.2 dimeglumine....

Brefeldin A (BFA), a well-known natural Arf-GEFs inhibitor, is effective against hepatocellular carcinoma (HCC), while the poor solubility, serious toxicity, and short half-life limit its potential. Herein, distinct corresponding prodrugs of BFA, including esters 1–15, carbonates 16–24 30–32, carbamates 25–29, were synthesized evaluated. CHNQD-01255 (16) with improved aqueous solubility (15–20 mg/mL) demonstrated favorable pharmacokinetic profiles. It behaved as expected by undergoing rapid...

Abstract Background To evaluate whether there are differences in the cerebral response to intraesophageal acid and psychological anticipation stimuli among subtypes of gastroesophageal reflux disease (GERD). Methods Thirty nine patients with GERD 11 healthy controls were enrolled this study after gastroscopy 24 hr pH monitoring. subjects divided into four subgroups: RE (reflux esophagitis), NERD+ (non-erosive excessive reflux), NERD-SI+ (normal exposure positive symptom index) negative...

Lentinula edodes, also known as Xiang'gu, is commonly eaten in cultures around the world. However, L. edodes particularly susceptible to enrichment with heavy metals, cadmium (Cd), which toxic human health. Understanding molecular mechanism and mining key genes involved Cd will facilitate genetic modification of strains. Two genotypes, Le4625 (with higher capability) Le4606 lower were used this study. The concentrations mycelia tested genotypes differed significantly after (0.1 mg/L)...

Reinforced concrete and steel structures are commonly used in bridge construction; thus, researchers have conducted relatively comprehensive analyses of their dynamic responses damage mechanisms under blast loads. However, for a new type I-shaped steel‒concrete composite structure with various structural performance advantages, there remains lack effective experiments modes. In this paper, three specimens designed fabricated, explosion different scenarios performed. The experimental results...

Post-myocardial infarction heart failure (post-MI HF) is one of the leading global causes death, and current prevention treatment methods still cannot avoid increasing incidence. Honokiol (HK) has previously been reported to improve myocardial ischemia/reperfusion injury reverse hypertrophy by activating Sirt1 Sirt3. We suspect that HK may also have a therapeutic effect on post-MI HF. In this study, we aimed investigate efficacy mechanism in found inhibited reactive oxygen species (ROS)...