Background Cardiac progenitor cells (CPCs) have been shown to be suitable in stem cell therapy for resurrecting damaged myocardium, but poor retention of transplanted the ischemic myocardium causes ineffective therapy. Hypoxic preconditioning can increase expression CXCR4 and pro-survival genes promote better survival; however, it is unknown whether hypoxia will influence survival CPCs via SDF-1α/CXCR4 axis. Methods Results were isolated from adult mouse hearts purified by magnetic activated...

Atherosclerosis (AS) is a chronic inflammatory disease threatening human health, and vascular smooth muscle cells (VSMCs) are involved in AS processes. Baicalin flavonoid compound, which has anti-atherosclerotic effect. The aim of our study was to explore the molecular mechanism baicalin on AS. expression miR-126-5p measured peripheral blood patients healthy control. We found decreased Then, high-mobility group box 1 (HMGB1) verified as target its increased Similarly, HMGB1 downregulated...

Kallistatin (KS) has been recognized as a plasma protein with anti-inflammatory functions. Macrophages are the primary inflammatory cells in atherosclerotic plaques. However, it is unknown whether KS plays role macrophage development and pathogenesis of atherosclerosis. This study investigated development, key pathological process An atherosclerosis model was established ApoE−/– mice via partial left carotid artery (PLCA) ligation. adenovirus vector (Ad. HKS) containing human gene delivered...

Background Acute myocardial infarction (AMI), a subset of acute coronary syndrome, remains the major cause mortality worldwide. Mitochondrial dysfunction is critically involved in AMI progression, and mitophagy plays vital role eliminating damaged mitochondria. This study aimed to explore mitophagy-related biomarkers their potential molecular basis AMI. Methods datasets (GSE24519 GSE34198) from Gene Expression Omnibus database were combined batch effects removed. Differentially expressed...

ObjectivesStem cell preconditioning (PC) is a powerful approach in reducing death after transplantation. We hypothesized that PC human endothelial progenitor cells (hEPCs) with bradykinin (BK) enhance survival, inhibit apoptosis and repair the infarcted myocardium. MethodsThe hEPCs were preconditioned or without BK. The induced by hypoxia along serum deprivation was determined annexin V-fluorescein isothiocyanate/ propidium iodide staining. Cleaved caspase-3, Akt eNOS expressions Western...

Purpose: Results for implantation efficiency and effective improvement of cardiac function in the field mesenchymal stem cells (MSCs) are controversial. To attempt to clarify this debate, we utilized magnetic resonance imaging (MRI) near-infrared optical (OI) explore effects different delivery modes on cell retention time after myocardial infarction (MI). Methods: Rat MSCs were labeled with superparamagnetic iron oxide nanoparticles 1, 1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine,...

Abstract Cardiac fibroblast (CF) differentiation to myofibroblasts expressing α‐smooth muscle actin (α‐SMA) plays a key role in cardiac fibrosis. Therefore, study of the mechanism regulating α‐SMA expression is means understanding and Previous studies have shown that DNA methylation associated with gene related development tissue However, mechanisms by which CF regulated remain unclear. Here, we explored epigenetic regulation its relevance differentiation. In this study, demonstrated was...

Background Angiotensin II (ANG II) promotes vascular inflammation and induces abdominal aortic aneurysm (AAA) in hyperlipidemic apolipoprotein E knock-out (apoE−/−) mice. The aim of the present study was to detect macrophage activities an ANG II-induced early-stage AAA model using superparamagnetic iron oxide (SPIO) as a marker. Methodology/Principal Findings Twenty-six male apoE−/− mice received saline or (1000 500 ng/kg/min) infusion for 14 days. All animals underwent MRI scanning...

Tissue kallikrein (TK) has been demonstrated to improve neovasculogenesis after myocardial infarction (MI). In the present study, we examined role and underlying mechanisms of TK in peripheral endothelial progenitor cell (EPC) function. Peripheral blood-derived mononuclear cells containing EPCs were isolated from rat. The vitro effects on EPC differentiation, apoptosis, migration, vascular tube formation capacity studied presence or absence TK, kinin B(2) receptor antagonist (icatibant),...

Abstract Objective MicroRNAs (miRNAs) have been shown to play crucial roles in the occurrence, development, and treatment of many cardiovascular diseases. Coronary heart disease (CAD)‐related miRNAs are still a growing research area. miR‐7b was reported be downregulated acute myocardial infarction (AMI) myocardium tissues. However, it remains largely unknown whether is involved pathogenesis progression AMI ischemia/reperfusion (I/R) injury. Methods Male C57BL/6 J mice H9C2 cells were used as...

Purpose: Pulmonary hypertension (PH) is a pathological process mainly characterized by the progressive increase in pulmonary vascular resistance. The degradation of artery smooth muscle cells (PASMCs) from contractile/differentiated phenotype to synthetic/dedifferentiated key factor for hypoxic hypertension. Materials and Methods: In this study, qPCR was performed evaluate gene expression mRNAs. Western blot, immunofluorescence RNA pull down were used detect levels. Results: We found that...

The peroxisome proliferator-activated receptor-γ (PPARγ) agonist rosiglitazone has been suggested to exert cardiovascular protection through the improvement of lipid metabolism, anti-inflammation, anti-proliferation etc. However, whether renin-angiotensin system (RAS) is involved in vascular protective effects PPARγ agonists not fully understood. present study aimed investigate mediated by agonists. To actions activation vivo and vitro. Rats were fed a regular diet (n = 8), cholesterol-rich...

Acute myocardial infarction (AMI) remains one of the leading causes mortality worldwide; however, endothelial progenitor cell (EPC) transplantation has been proposed as a promising treatment strategy for EPC. High levels tumor necrosis factor-related weak inducer apoptosis (TWEAK) have reported in AMI, although its effect on EPCs not reported. In present study, immunofluorescence and flow cytometry were performed to assess TWEAK isolated mouse EPCs. Echocardiography was used evaluate cardiac...

Ischaemic heart disease is one of the leading causes death. Protease-activated receptor 2 (PAR2) widely expressed within cardiovascular system and known to mediate inflammatory processes in various immunocytes, such as macrophages, mastocytes neutrophils. Here, we investigated whether activating macrophage PAR2 modulates cardiac remodelling a murine model myocardial infarction. Myocardial infarction was produced by permanent ligation left anterior descending coronary artery (LAD) C57BL/6J...

Abstract Cardiac hypertrophy is not only an adaptational state before heart failure but also independent risk factor for ischemia, arrhythmia, and sudden death. However, the direct effects of hypercholesterolemia on myocardium mechanisms are completely understood. It has been demonstrated that peroxisome proliferator‐activated receptor‐ γ (PPAR ) ligand agonists attenuate cardiac through anti‐inflammatory effects. The present study investigated PPAR hypercholesterolemia‐dependent,...

Background Acute myocardial infarction (AMI) is necrosis caused by acute and persistent ischemia hypoxia of coronary arteries. AMI one the most common diseases in European countries over 1.5 million patients die it United States annually. A collection studies proposed that certain micro-RNAs play crucial roles onset development AMI. Methods Ninety-four 83 non-AMI healthy controls were recruited from Zhongda Hospital, Southeast University between July 2015 September 2017. Serum samples...

Advanced glycation end products of BSA (AGE-BSA) contribute to the pathogenesis diabetic vascular diseases. However, roles and underlying mechanisms AGE-BSA in diseases remain largely unclear. Long non-coding RNAs (lncRNAs) are widely identified known as gene regulators. lncRNAs disease still vague. In this study, we sought investigate contributions human umbilical vein endothelial cells (HUVECs) treated with AGE-BSA. We first demonstrated that reduced cell viability inhibited proliferation...

Ly6Chigh monocytes are inflammatory cells that accumulate in an infarcted myocardium, and Ly6Clow believed to be reparative curb myocardial remodeling. NR4A1 is a novel target for modulating the phenotype of during atherogenesis. We aimed investigate whether MSCs can contribute heterogeneity differentiated into this regulation related nuclear receptor NR4A1. Ly6Chigh/low were first cocultured with MSCs. C57BL/6CX3CR1-/- mice C57BL/6 wild-type then used construct AMI models, survival...