A5102964336- Herpesvirus Infections and Treatments
- Poxvirus research and outbreaks
- Bacillus and Francisella bacterial research
- Rabies epidemiology and control
- Liver Disease Diagnosis and Treatment
- Virus-based gene therapy research
- Diet and metabolism studies
- Diet, Metabolism, and Disease
- Stress Responses and Cortisol
- Zoonotic diseases and public health
- Neuroendocrine regulation and behavior
- Tryptophan and brain disorders
- Cytomegalovirus and herpesvirus research
- Health and Medical Research Impacts
- Innovations in Medical Education
- Viral Infections and Outbreaks Research
- Animal Disease Management and Epidemiology
- Virology and Viral Diseases
- Regulation of Appetite and Obesity
- Career Development and Diversity
- Pharmacological Effects of Natural Compounds
- Estrogen and related hormone effects
Salk Institute for Biological Studies
2024
Centers for Disease Control and Prevention
2017-2021
Ministry of Public Health
2019
University of Kinshasa
2019
National Institute of Biomedical Research
2019
Ministère de l'Agriculture
2019
Oak Ridge Associated Universities
2019
Oak Ridge Institute for Science and Education
2019
Instituto Nacional de Salud
2019
Universidad Cooperativa de Colombia
2019
Monkeypox, caused by a zoonotic orthopoxvirus, is endemic in Central and West Africa. Monkeypox has been sporadically reported the Republic of Congo. During March 22-April 5, 2017, we investigated 43 suspected human monkeypox cases. We interviewed case-patients collected dried blood strips vesicular crust specimens (active lesions), which tested for orthopoxvirus antibodies ELISA virus varicella zoster DNA PCR. An ecologic investigation was conducted around Manfouété, from 105 small mammals...
Monkeypox virus (MPXV) is a human pathogen that member of the Orthopoxvirus genus, which includes Vaccinia and Variola (the causative agent smallpox). Human monkeypox considered an emerging zoonotic infectious disease. To identify host factors required for MPXV infection, we performed genome-wide insertional mutagenesis screen in haploid cells. The revealed several candidate genes, including those involved Golgi trafficking, glycosaminoglycan biosynthesis, glycosylphosphatidylinositol...
Human infection by orthopoxviruses is being reported with increasing frequency, attributed in part to the cessation of smallpox vaccination and concomitant waning population-level immunity. In July 2015, a female resident interior Alaska presented an urgent care clinic dermal lesion consistent poxvirus infection. Laboratory testing virus isolated from confirmed Orthopoxvirus.The isolate was characterized using electron microscopy nucleic acid sequencing. An epidemiologic investigation that...
Monkeypox virus is a zoonotic Orthopoxvirus (OPXV) that causes smallpox-like illness in humans. In Cameroon, human monkeypox cases were confirmed 2018, and outbreaks captive chimpanzees occurred 2014 2016. We investigated the OPXV serological status among staff at primate sanctuary (where 2016 chimpanzee outbreak occurred) residents from nearby villages, describe contact with possible reservoirs. focused specifically on Gambian rats ( Cricetomys spp.) because they are recognized reservoirs...
Smallpox vaccine is contraindicated in immunosuppression due to increased risk for adverse reactions (eg, progressive vaccinia). We describe the first-ever use of tecovirimat as a preemptive vaccinia virus treatment strategy during induction chemotherapy an active duty service member who presented with acute leukemia and inadvertent autoinoculation after smallpox vaccination.
Decades after the eradication of smallpox and discontinuation routine vaccination, over half world's population is immunologically naïve to variola virus other orthopoxviruses (OPXVs). Even in those previously vaccinated against smallpox, protective immunity wanes time. As such, there a concomitant increase incidence human OPXV infections worldwide. To identify novel antiviral compounds with potent anti-OPXV potential, we characterized inhibitory activity PAV-866 methylene blue derivatives...
Kalanchoe pinnata (Lam.) Pers. (Crassulaceae) is a succulent plant that known for its traditional antivirus and antibacterial usage.This work examines two compounds identified from the K. their activity against human alphaherpesvirus (HHV) 1 2 vaccinia virus (VACV).Compounds KPB-100 KPB-200 were isolated using HPLC NMR MS. Both tested in plaque reduction assay of HHV-2 wild type (WT) VACV. then spread inhibition yield (VYR) assays was further viral cytopathic effect (CPE) TK-mutant VYR HHV-1...
In 2014, vaccinia virus (VACV) infections were identified among farmworkers in Caquetá Department, Colombia; additional cases Cundinamarca Department 2015. VACV, an orthopoxvirus (OPXV) used the smallpox vaccine, has caused sporadic bovine and human outbreaks countries such as Brazil India. response to emergence of this disease Colombia, we surveyed collected blood from 134 household members 56 farms Department. We tested serum samples for OPXV antibodies correlated risk factors with...
In March 2015, a patient in Colombia with HIV/AIDS was hospitalized for disseminated ulcers after milking cows that had vesicular lesions on their udders. Vaccinia virus detected, and the case met criteria progressive vaccinia acquired by zoonotic transmission. Adherence to an optimized antiretroviral regimen resulted recovery.
The effectiveness of rabies vaccination in both humans and animals is determined by the presence virus neutralizing antibodies (VNAs). Rapid Fluorescent Focus Inhibition Test (RFFIT) method traditionally used for detection quantification VNAs. It a functional vitro test assessing ability serum to bind prevent infection cultured cells with (RABV). RFFIT labor intensive, low throughput semi-quantitative assay performed trained laboratorians. requires staining RABV-infected specific fluorescent...
Cerebral glucose uptake is mediated by several members of the family facilitated transporters (protein nomenclature GLUT; gene solute carrier 2 Slc2a). Glucose differs between sexes and also varies with menstrual status in women across rodent oestrous cycle. The present study demonstrates extent to which hormonal variation four stages rat cycle affects mRNA abundance GLUT family, including most well characterised cerebral Slc2a1 Slc2a3, as insulin-sensitive Slc2a4 Slc2a8 hypothalamus,...