A5069905041- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Ferroptosis and cancer prognosis
- Toxin Mechanisms and Immunotoxins
- Blood properties and coagulation
- Nanoplatforms for cancer theranostics
- Immune cells in cancer
- RNA Research and Splicing
- Viral Infectious Diseases and Gene Expression in Insects
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- Venomous Animal Envenomation and Studies
- MicroRNA in disease regulation
- Transgenic Plants and Applications
- Biochemical and Structural Characterization
- Autophagy in Disease and Therapy
- Vitamin K Research Studies
- Cloud Data Security Solutions
- Protein purification and stability
University of Science and Technology of China
2014-2024
Jiangmen Central Hospital
2024
Zhejiang University
2024
École Polytechnique Fédérale de Lausanne
2018-2024
Jinhua Academy of Agricultural Sciences
2024
Nanyang Normal University
2020-2023
Taiyuan University of Technology
2019
Electric Power Research Institute
2019
Wuhan University of Technology
2018
Center for Excellence in Molecular Cell Science
2017
Abstract Ferroptosis is closely linked to various cancers, including lung adenocarcinoma (LUAD); however, the factors involved in regulation of ferroptosis-related genes are not well established. In this study, we identified and characterized long noncoding RNAs (lncRNAs) LUAD. particular, a coexpression network mRNAs lncRNAs from The Cancer Genome Atlas (TCGA) was constructed. Univariate multivariate Cox proportional hazards analyses were performed establish prognostic lncRNA signature...
Abstract The efficacy of adoptive T cell therapy (ACT) for the treatment solid tumors remains challenging. In addition to poor infiltration effector (Teff) cells limited by physical barrier surrounding tumor, another major obstacle is extensive regulatory (Treg) cells, a immunosuppressive immune subset, in tumor microenvironment. Here, this work develops grooved microneedle patch augmenting ACT, aiming simultaneously overcome and barriers. microneedles are engineered through an ice‐templated...
Abstract Targeting the stimulator of interferon genes (STING) pathway with cyclic dinucleotides (CDNs), natural STING agonists, is a promising immunotherapeutic strategy for cancer. However, clinical application CDNs as therapeutics greatly hindered by their intrinsic properties including negative charges, small molecular weight, and high susceptibility to enzymatic degradation. Mn 2+ ions have been recently discovered directly activate GMP‐AMP (cGAMP) synthase (cGAS) augment cGAMP‐STING...
Cerebral ischemia-reperfusion injury (CIRI) is an important pathophysiological process of ischemic stroke associated with various physiological and pathological processes, including autophagy apoptosis. In this study, we examined the role mechanism long noncoding RNA CAMK2D-associated transcript 2 (C2dat2) in regulating CIRI vivo vitro. C2dat2 up-regulation facilitated neuronal apoptosis induced by CIRI. Mechanistically, acts as a competing endogenous (ceRNA) to negatively regulate...
Immune stimulatory antibodies and cytokines elicit potent antitumor immunity. However, the dose-limiting systemic toxicity greatly hinders their clinical applications. Here, we demonstrate a chemical approach, termed “switchable” immune modulator (Sw-IM), to limit exposure therefore ameliorate toxicities. Sw-IM is biomacromolecular therapeutic reversibly masked by biocompatible polymers through linkers that are responsive tumor-specific stimuli, such as high reducing potential acidic pH....
Background The human insulin-like growth factor 2 mRNA binding proteins 1–3 (IGF2BP1–3, also called IMP1–3) play essential roles in regulation, including its splicing, translocation, stability, and translation. However, knowledge regarding the involvement of IGF2BPs tumor immunity stemness across cancer types is still lacking. Methods In this study, we comprehensively analyzed pan-cancer multi-omic data to determine correlation protein expression with various parameters such as mutation...
The yeast Pichia pastoris (P. pastoris) has become a popular `cell factory' for producing heterologous proteins, but production widely varies among proteins. Cultivation temperature is frequently reported to significantly affect protein production; however, the underlying mechanisms of this effect remain unclear. A P. strain expressing recombinant human interleukin-10 (rhIL-10) under control AOX1 promoter was used as model in study. This system shows high-yield rhIL-10 with prolonged...
Natural killer (NK) cells, a type of potent cytotoxic lymphocytes, are particularly promising for the treatment cancers that lose or downregulate major histocompatibility complex class I (MHC-I) expression to evade T cell-mediated immunotherapy. However, hostile and immune suppressive tumor microenvironment (TME) greatly hinders function tumor-infiltrating NK cells limiting therapeutic efficacy against solid tumors. Here, we show fusion protein interleukin-21 (IL-21−Fc), as direct in vivo...
Abstract Tumour-induced dendritic cell (DC) dysfunction plays an important role in cancer immune escape. However, the underlying mechanisms are not yet fully understood, reflecting lack of appropriate experimental models both vivo and vitro . In present study, study model for tumour-induced DC was established by culturing DCs with pooled sera from multiple non-small lung (NSCLC) patients. The results demonstrated that human monocyte-derived exhibited systematic functional deficiencies....
Abstract Hematopoietic progenitor pinase1 (HPK1) knockout has been identified as an efficient route to enhance anti‐tumor immune response. Here, this work develops oral proteolysis targeting chimera (PROTAC) HPK1 efficiently and selectively degrade augment immunotherapeutic outcomes. In a postoperative tumor model of human cervical cancer in NSG mice, the orally‐administrated PROTAC can reach tumors, down‐regulate levels locally‐administrated CAR‐T cells, promote their efficiency inhibiting...
Paired box protein 5 (PAX5) plays a lineage determination role in B-cell development. However, high expression of PAX5 has been also found various malignant diseases, including B-lymphoproliferative disorders (B-LPDs), but its functions and mechanisms these diseases are still unclear. Here, we show that induces drug resistance through association activation receptor-interacting serine/threonine-protein kinase 2 (RIP2; known as RIPK2), subsequent NF-κB signaling anti-apoptosis gene cells....
The microbial transglutaminase (MTG) is inactive when only the mature sequence expressed in Pichia pastoris. Although co-expression of MTG and its N-terminal pro-peptide can obtain active MTG, enzyme activity was still low. One basic steps for strain improvement to ensure a sufficient level transcription heterologous gene, based on promoter strength gene copy number. To date, high-copy-number recombinants P. pastoris are achievable by cloning concatemers, so methods rapid reliable multicopy...
The highly conserved homology cassette family (HOX) as well 18 referenced long non-coding antisense transcripts (HOXATs) play vital roles in the development of some cancers. Nevertheless, their expression patterns association with cancer prognosis and tumor microenvironment (TME) pan-cancers are still unclear. Here, based on public databases, levels HOXATs, prognostic potentials, correlation mutation burden (TMB), immune cell infiltration, subtype, response-related genes, stemness scores...
CD83 is a highly glycosylated type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. upregulated during dendritic cell (DC) maturation, which critical for initiation of adaptive immune responses. The soluble isoform (sCD83) encoded by alternative splicing from full-length mRNA and inhibits DC suggests sCD83 acts as potential suppressor. In this study, we developed sound strategy express functional Pichia pastoris in extremely high-density fermentation. Purified was...
Agkisacucetin extracted from the venom of Agkistrodon acutus has been demonstrated to be a promising antithrombotic drug candidate in clinical studies due its function as novel platelet membrane glycoprotein (GP) Ib inhibitor. is heterodimeric protein composed α- and β-subunits with seven disulphide bonds. Both subunits form inactive homodimeric products, which cause difficulties for recombinant production. In this study, were inserted sequentially into chromosome Pichia pastoris at mutant...
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are RNA-binding proteins that reported to play a crucial role in the pathogenic process of multiple malignancies. However, their expression patterns, clinical application significance and prognostic values invasive breast carcinoma (BRCA) remain unknown. In this study, we investigated hnRNP family members BRCA using accumulated data from Oncomine 4.5, UALCAN Web portal other available databases. We explored value level hnRNPs BRCA. further...