The aim of this study was to explore the association specific microRNAs (miRNAs) with development esophageal cancer (EC) and identify new molecular markers for EC by analyzing expression profiles miRNAs in tissues. paired paracancerous normal tissues were detected bioinformatically analyzed using miRNA assays. outcomes validated real-time polymerase chain reaction. assays revealed a total 60 differentially expressed compared those Among them, 51 had doubled or more than their levels 9 halved...

Abstract Background Leaves of the natural plant lotus are used in traditional Chinese medicine and tea production. They rich flavonoids. Methods In this study, leaf flavonoids (LLF) were applied to human lung cancer A549 cells small cell H446 vitro verify effect LLF on apoptosis these through ROS/p38 MAPK pathway. Results had no toxic normal at concentrations up 500 µg/mL, but could significantly inhibit proliferation cells. Flow cytometry showed that induce growth We also found increase ROS...

Single-nucleotide polymorphisms in microRNAs (miRNAs) may dramatically affect gene expression and subsequently alter individual susceptibility to cancer, thus has become a research hotspot for many cancer types, including breast cancer. We recruited 321 patients 290 controls our study. Four established miRNA single-nucleotide (mir-499 rs3746444 A>G; miR-27a rs895819 miR-196a2 rs11614913 T>C; miR-146a rs2910164 G/C) were detected using Taqman assays. Mature expression, allele distribution,...

CircPVT1 is demonstrated to promote cancer progression in esophageal squamous cell carcinoma (ESCC). However, the role and potential functional mechanisms of circPVT1 regulating 5-fluorouracil (5-FU) chemosensitivity remain largely unknown.ESCC cells resistant 5-FU were induced with continuous increasing concentrations step-wisely. A counting kit-8 assay was used analyze viability ESCC cells. LDH release kit evaluate cytotoxicity. RT-qPCR assess expression level non-coding RNAs cDNAs....

Esophageal squamous carcinoma (ESCC) is a common malignancy that originates in the digestive tract. Lymph node metastasis (LNM) complicated process, and tumor lymphangiogenesis has been reported to be associated with spread of cells lymph nodes (LNs), including ESCC. However, little currently known about mechanisms involved ESCC tumors. According previous literature, we know hsa_circ_0026611 expresses at high level serum exosomes patients shows close association LNM poor prognosis. details...

Accumulating evidence has shown that microRNAs are involved in cancer development and progression. However, it remains unknown about the potential role of miR-19a pathogenesis gastric cancer. Here, we report suppressor cytokine signaling 1 (SOCS1) is a novel target cells expression inversely correlated with SOCS1 subset tissues. Ectopic dramatically promoted proliferation tumorigenicity both vitro vivo. Moreover, showed silencing cell growth colony formation resembling overexpression,...

The aim of this study is to investigate whether metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1) can be used as a potential therapy target for human esophageal squamous cell carcinoma. MALAT-1 expression levels were detected in 137 paired EC samples and adjacent nonneoplastic tissues. Human carcinoma lines EC9706 KYSE150 transfected with small interference RNA. Cell proliferation, migration/invasion ability, cycle, apoptosis assessed. expressed higher cancer tissues when...

ABSTRACT Dysregulation of m6A modification has emerged as a vital factor in the development esophageal squamous cell carcinoma (ESCC). Here, we sought to explore critical role methylation mediated by methyltransferase METTL3 ESCC. Protein expression analysis was performed immunohistochemistry and immunoblot assays. The mRNA levels IFI27 were detected quantitative PCR. Cell sphere formation potential, migration, invasiveness, apoptosis, proliferation viability assessed standard formation,...

The treatment of cancer has brought about a paradigm shift with the introduction immune checkpoint blockade (ICB) therapy, which is mostly dependent on inhibiting PD-1/PD-L1 and CTLA-4. However, recent studies have shown limited efficacy this in esophageal squamous cell carcinoma (ESCC). Preliminary found that tifcemalimab (the world's first anti-BTLA blocking monoclonal antibody) combined toripalimab (PD-1) chemotherapy favorable safety several solid cancers. This study aimed to evaluate...

e20510 Background: Multiple clinical trials have reported the striking benefit for PD-1/PD-L1 monoclonal antibody combined with chemotherapy neoadjuvant therapy non-small cell lung cancer (NSCLC), while efficacy of subcutaneous injection anti-PD-L1 Envafolimab NSCLC patients has not been demonstrated yet. is a novel recombinant protein humanized camel-derived single-domain fused human IgG1 Fc fragment designed injection, making it easy to administer and improving patient compliance. This...

This study aimed to investigate whether the miR-198 expression level is related clinicopathological factors and prognosis of esophageal cancer.MicroRNA was extracted from cancer patients who underwent surgery for assessment using Taqman@ MicroRNA assay. The correlation between features analyzed, significance as a prognostic factor its relationship with survival determined.MicroRNA-198 (miR-198) higher in poor than those good (P < 0.05). Kaplan-Meier analysis results showed that had...

AIM: To study the potential prognostic role of in esophageal squamous cell carcinoma (ESCC). METHODS:Forty six patients were divided into 2 groups according to postoperative survival time: poor outcome group (28 patients), who showed early metastasis but no recurrence, and died within 1 year after surgery, 12 received chemotherapy treatment that was given happening; good (18 had clinical survived 5 years or more all did not receive any treatment.Total RNA extracted from patients'...

There have been few reports evaluating the expression and function of microRNA miR-212 in esophageal cancer. The aim this study was to investigate relationship between clinicopathological factors prognoses MicroRNA extracted from 46 cancer patients using Taqman assay. All were at same tumor node metastasis stage, but with different prognoses, had all undergone surgery. correlation features analyzed significance as a prognostic factor well its survival determined. higher poor than those good...

We conducted a perspective study to investigate whether the expression of excision repair cross-complementing 1 (ERCC1), xeroderma pigmentosum group G (XPG), breast cancer (BRCA1), and ribonucleotide reductase M1 (RRM1) is correlated with clinical outcome non-small cell lung (NSCLC). Patients histologically confirmed inoperable stages IIIB IV NSCLC were collected followed up until January 2012. Relative cDNA quantification for ERCC1, XPG, BRCA1, RRM1 was performed using fluorescence-based,...

The aim of this study was to investigate the significance microRNA miR-197 expression level in relation clinicopathological factors and prognoses esophageal cancer (EC).MicroRNA extracted using Taqman ® MicroRNA Assay from 46 EC patients at same tumor node metastasis (TNM) stage, but with different prognoses, who underwent surgery.Paracancerous normal tissues were used as controls.The correlation between clinicopathologic features analyzed, a prognostic factor its relationship survival...

The aim of this study was to observe the preventive effect flavonoids extracted from Malus asiatica Nakai leaves (FMANL) on esophageal cancer in mice, especially ability FMANL regulate interleukin 17 (IL-17) signaling pathway during process.The C57BL/6J mice were treated with 4-nitroquinoline N-oxide (4NQO) induce cancer, and visceral tissue index serum indexes used verify FMANL.The experimental results showed that can effectively control changes caused by cancer. could increase cytokine...

Excessive inflammatory response and apoptosis play key roles in the pathogenic mechanisms of sepsis‑induced acute lung injury (ALI); however, molecular pathways linked to ALI pathogenesis remain unclear. Recently, microRNAs (miRNAs/miRs) have emerged as important regulators inflammation ALI; exact regulatory miRNAs poorly understood. In present study, gene microarray dataset GSE133733 obtained from Gene Expression Omnibus database was analyzed a total 38 differentially regulated were...

This study aimed to explore the role of micorRNA-2053 in esophageal cancer development. The expression level miR-2053 cell lines was detected. After transfection, effects overexpression on proliferation, apoptosis, migration and invasion cells were determined. Moreover, potential molecular mechanism explored by measuring epithelial-mesenchymal transition (EMT) apoptosis-related proteins. Luciferase reporter assay conducted investigate target gene miR-2053. protein expressions PI3K/AKT...