A5072370023- Ovarian cancer diagnosis and treatment
- Endometrial and Cervical Cancer Treatments
- Endometriosis Research and Treatment
- Venous Thromboembolism Diagnosis and Management
- Uterine Myomas and Treatments
- Hepatitis B Virus Studies
- Blood Coagulation and Thrombosis Mechanisms
- Cervical Cancer and HPV Research
- Intraperitoneal and Appendiceal Malignancies
- Inflammatory Biomarkers in Disease Prognosis
- Gynecological conditions and treatments
- Liver Disease Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- Helicobacter pylori-related gastroenterology studies
- Acute Myeloid Leukemia Research
- Glycosylation and Glycoproteins Research
- Nausea and vomiting management
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Cancer Diagnosis and Treatment
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Reproductive System and Pregnancy
- Veterinary medicine and infectious diseases
- Cancer-related molecular mechanisms research
- Monoclonal and Polyclonal Antibodies Research
Nara Medical University
2016-2025
Kyushu University
2025
Nara Medical University Hospital
2011-2024
Weatherford College
2008
Genomics (United Kingdom)
2002-2003
Genesis Research Institute
2003
St. Marianna University School of Medicine
1997
Tokyo Women's Medical University
1995
Tokyo University of Agriculture and Technology
1992-1994
ABSTRACT Quantitation of hepatitis B virus (HBV) DNA in serum is a useful method for the monitoring HBV replication. We attempted to develop quantitative assay system that more sensitive, accurate, and reproducible than existing systems. detected by real-time detection PCR (RTD-PCR) based on Taq Man chemistry. The efficacy this was evaluated quantitatively measuring sequential levels synthetic clinical samples. limit as few 10 copies/reaction. A linear standard curve obtained between 1 8...
Ovarian cancer is common in women from developed countries. We designed a prospective randomized controlled trial of ovarian screening to establish an improved strategy for the early detection cancers. Asymptomatic postmenopausal were randomly assigned between 1985 and 1999 either intervention group (n = 41,688) or control 40,799) ratio 1:1, with follow-up mean 9.2 years, Shizuoka district, Japan. The original intention was offer annual screens by gynecological examination (sequential pelvic...
ABSTRACT Phylogenetic analysis of about 200 strains Salmonella , Shigella and Escherichia coli was carried out using the nucleotide sequence gene for DNA gyrase B ( gyrB ), which determined by directly sequencing PCR fragments. The results establish a new phylogenetic tree classification in forms cluster separate from but closely related to E. coli. In comparison with 16S rRNA analysis, sequences indicated greater evolutionary divergence bacteria. Thus, screening presence bacteria, might be...
Six years after the first edition of The Guideline for Gynecological Practice, which was jointly edited by Japan Society Obstetrics and Gynecology Association Obstetricians Gynecologists, third revised published in 2017. 2017 Guidelines includes 10 additional clinical questions (CQ), brings total to 95 CQ (12 on infectious disease, 28 oncology benign tumors, 27 endocrinology infertility healthcare women). Currently a consensus has been reached therefore objective this report is present...
ABSTRACT Rapid identification of Mycobacterium species isolates is necessary for the effective management tuberculosis. Recently, analysis DNA gyrase B subunit ( gyrB ) genes has been identified as a suitable means bacterial species. We describe microarray assay based on gene sequences that can be used Mycobacteria Primers specific region common to all mycobacteria were synthesized and PCR amplification purified from clinical samples. A set oligonucleotide probes regions was developed 14...
A novel cell line SKNO‐1 was established from the bone marrow cells of a 22‐year‐old male suffering acute myeloblastic leukaemia (AML) M2 with t(8;21) whose disease became resistant to chemotherapy after acquisition 17 monosomy. has been maintained for more than 36 months as granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) dependent line. Morphologically, were myeloblasts somewhat maturated. The grow in suspension doubling time 48‐72 h. survival and growth absolutely on colony...
Abstract Commonly, 16S ribosome RNA (16S rRNA) sequence analysis has been used for identifying enteric bacteria. However, it may not always be applicable distinguishing closely related Therefore, we selected gyrB genes that encode the subunit B protein of DNA gyrase (a topoisomerase type II protein) as target genes. The molecular evolution rate is higher than rRNA, and are distributed universally among bacterial species. Microarray technology includes methods arraying cDNA or...
Mural nodules and papillary projections can be seen in benign ovarian endometriosis (OE) malignant transformation of OE (endometriosis-associated cancer [EAOC]), which pose a challenging diagnostic dilemma to clinicians. We identify the preoperative imaging characteristics helpful differential diagnosis between with mural EAOC.This was retrospective study 82 patients who were diagnosed pathologically have (n = 42) transformations these tumors 40) at Nara Medical University Hospital from...
Abstract Nine years after the first edition of The Guideline for Gynecological Practice, which was jointly edited by Japan Society Obstetrics and Gynecology Association Obstetricians Gynecologists, 4th Revised Edition published in 2020. 2020 Guidelines includes 4 additional clinical questions (CQ), brings total to 99 CQ (12 on infectious disease, 29 oncology benign tumors, endocrinology infertility healthcare women). Currently, a consensus has been reached Guidelines, therefore, objective...
We have investigated the evolutionary relationships between two facultatively anaerobic Magnetospirillum strains (AMB-1 and MGT-1) fastidious, obligately microaerophilic species, such as magnetotacticum, using a molecular phylogenetic approach. Genomic DNA from MGT-1 AMB-1 was used template for amplification of genes coding 16S rRNA (16S rDNA) by polymerase chain reaction. Amplified fragments were sequenced (1,424 bp) compared with sequences M. magnetotacticum MS-1 gryphiswaldense MSR-1....
The isolation and identification of the 87A strain hepatitis E virus (HEV) by means cell culture have been described previously. This paper reports nucleotide sequence a portion this HEV strain. RNA extracted from supernatants different passages cultured in A549 line was reverse-transcribed (RT) to cDNA, then polymerase chain reaction (PCR) amplification carried out using primers ET1.1 region. PCR products 1) supernatant infected cells at fourth passage, 2) concentrated polyethylene glycol...
The purpose of this study was to describe the clinicopathologic features malignant transformation in patients with ovarian endometrioma, their treatment and outcome Kinki region, Japan.Patients meeting Sampson Scott's criteria for cancer associated endometriosis were identified by chart review pathology reports. This retrospective survey describes 18 women a history endometrioma. These cases followed as benign endometrioma before on basis imaging findings gynecologic oncologists or...
The purpose of the present study was to elucidate incidence deep venous thrombosis (DVT) before treatment in ovarian cancer and appropriate cut-off value D-dimer (DD) for diagnosis DVT.Between July 2007 October 2008, eighty seven patients with presumed (final diagnosis: cancer, n=59; borderline malignancy, n=28) were enrolled. Measurement DD levels subsequent ultrasonography performed treatment.The mean level 4.1 µg/mL. Subsequent revealed DVT 14 87 (16.1%) (ovarian 12 cases; 2 cases). None...
<title>Abstract</title> Ovarian clear cell carcinoma is characterized by HNF-1ß overexpression and known to be resistant chemotherapy. An inhibitor screening that specifically targets led us identify Actinonin as a candidate for cancer treatment. Actinonin, which inhibit aminopeptidase M, has also been recognized its antibacterial properties. We confirmed GSK-3ß interference/inhibition, component of the pathway, combined with highly potent antitumor effect compared monotherapy.The same was...
Abstract A strain of hepatitis E virus (HEV), the 87A isolated in 2BS cells from feces a patient with E, has been reported previously. In this study, was propagated A549 cells, and marked cytopathic effect (CPE) appeared infected monolayer cells. The size is about 30 nm diameter. Furthermore, HEV‐RNAfrom supernatants different passages detected by polymerase chain reaction (PCR) amplification using ET1 .1 HEV primers. band for 239 bp PCR products revealed electro‐phoresis. fourth passage...
Most of the 16S ribosomal RNA gene a sulfate-reducing magnetic bacterium, RS-1, was sequenced, and phylogenetic analysis carried out. The results suggest that RS-1 is member delta-Proteobacteria, it appears to represent new genus. first bacterium reported outside alpha-Proteobacteria contains magnetite inclusions. therefore disrupts correlation between possession inclusions, delta-Proteobacteria greigite existence also suggests intracellular biomineralization multiple evolutionary origins.
In the present study, we evaluated changes in CA-125 cut-off values predictive of complete interval debulking surgery (IDS) after neoadjuvant chemotherapy (NAC) using receiver operating characteristic (ROC) analysis.This retrospective single-institution study included patients with International Federation Gynecology and Obstetrics (FIGO) stage III epithelial ovarian cancer a pre-NAC serum level greater than 40 U/mL who were treated platinum-based followed by IDS between 1994 2009. Logistic...