A5101990124- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Hepatocellular Carcinoma Treatment and Prognosis
- Liver physiology and pathology
- Circular RNAs in diseases
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Data Mining Algorithms and Applications
- Cancer, Hypoxia, and Metabolism
- Pediatric Hepatobiliary Diseases and Treatments
- interferon and immune responses
- Tissue Engineering and Regenerative Medicine
- Hepatitis C virus research
- Pancreatic and Hepatic Oncology Research
- Composite Structure Analysis and Optimization
- Immune cells in cancer
- Big Data Technologies and Applications
- Imbalanced Data Classification Techniques
- Mine drainage and remediation techniques
- Liver Diseases and Immunity
- RNA modifications and cancer
- Biliary and Gastrointestinal Fistulas
- Structural Analysis and Optimization
Third Xiangya Hospital
2005-2025
Central South University
2011-2025
Yunnan Agricultural University
2025
Xiangya Hospital Central South University
2015-2023
China National Petroleum Corporation (China)
2021
Research Institute of Petroleum Exploration and Development
2010-2021
Chengdu University of Technology
2010
Feed Research Institute
2008-2009
Chinese Academy of Agricultural Sciences
2008-2009
Biotechnology Research Institute
2008
The most common malignant tumor of the digestive system is HCC. However, mechanism and pathogenesis HCC occurrence progress are still unknown. LncRNA closely related to It important investigate effect role lncRNA in HCC.LncRNA microarray assay was used screen differential expression profile lncRNA. SNHG11, miR-184 GO2 analyzed by RT-PCR. ability SNHG11 serve as a sponge for miRNA fact that directly targets mRNA were revealed dual luciferase RIP. Apoptosis autophagy proteins detected Western...
Clinically significant portal hypertension (CSPH) is associated with an incremental risk of esophageal varices and overt clinical decompensations. However, hepatic venous pressure gradient (HVPG) measurement, the gold standard for defining CSPH (HVPG≥10 mm Hg) invasive therefore not suitable routine practice. This study aims to develop validate a radiomics-based model as noninvasive method accurate detection in cirrhosis.The prospective multicenter diagnostic trial (CHESS1701,...
Abstract Hepatocellular carcinoma is one of the most common solid tumors in digestive system. The prognosis patients with hepatocellular still poor due to acquisition multi-drug resistance. TNF Related Apoptosis Inducing Ligand (TRAIL), an attractive anticancer agent, exerts its effect selectively inducing apoptosis tumor cells through death receptors and formation downstream death-inducing signaling complex, which activates apical caspases 3/8 leads apoptosis. However, are resistant TRAIL....
Abstract Hepatocellular carcinoma (HCC) is the fifth most common cancer worldwide. LncRNA small nucleolar RNA host gene 14 (SNHG14) functions as an oncogene in a variety of cancers. However, role SNHG14 HCC remains elusive. The aim this study to unravel functional and regulatory mechanism HCC. A cohort 40 tumor tissues paired adjacent normal were collected. Histopathological changes analyzed by hematoxylin eosin immunohistochemistry. qRT-PCR western blotting performed determine levels...
Liver fibrosis is a common response to chronic liver injury, ultimately leading cirrhosis. The activation of hepatic stellate cells (HSCs) plays dominant role in fibrosis. regulatory roles long noncoding RNAs (lncRNAs) multiple human diseases have been observed. This study was dedicated investigating the effects lncRNA nuclear paraspeckle assembly transcript 1 (Neat1) on and HSC activation. Upregulation Neat1 cytohesin 3 (Cyth3) downregulation miR-148a-3p miR-22-3p were observed mouse...
Inflammation plays an essential role in the development liver fibrosis.The Cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS) is a central cytoplasmic DNA sensor which can recognize DNA, known to trigger stimulator of interferon genes (STING) and downstream proinflammatory factors. Here, we investigated cGAS-STING signaling pathway pathogenesis fibrosis.Differentially expressed (DEGs) human tissue were identified using RNA-Seq analysis. As models fibrosis, chronic Carbon...
An aerobic, Gram-stain-positive, rod-shaped and non-motile bacterium, designated strain P10A9, was isolated from the rhizosphere soil of Pu-erh tea plants ( Camellia sinensis var. assamica ) in an organic garden Jingmai Tea District, Pu'er City, Yunnan Province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that P10A9 belongs to genus Sinomonas , with its closest relative being atrocyanea KCTC 3377 T (98.4% similarity). The major fatty acids (>10.0% total) were...
EUS-guided portal pressure gradient (EUS-PPG) measurement has been proposed as a novel direct manometry to quantify hypertension. This study aimed explore the ability of EUS-PPG measurements evaluate severity The clinical features patients with diagnosed cirrhosis or chronic liver disease who underwent at single center were retrospectively analyzed. correlations between hypertension and analyzed, then receiver operating characteristic curves used severity. A total 197 included in this study....
An alkaliphilic actinobacterium, designated strain CAAS 252(T), was isolated from the black liquor treatment system of a cotton pulp mill in Wuhan, China. Cells 252(T) were Gram-positive, non-motile, non-endospore-forming, short rod-shaped, and grew optimally at 42 degrees C pH 9-10 presence 3 % (w/v) NaCl. Strain contained MK-7, MK-8 MK-9 as major menaquinones anteiso-C(17 : 0), anteiso-C(15 0) C(16 predominant cellular fatty acids had peptidoglycan type A4alpha, Lys-Gly-d-Asp. The DNA G+C...
A Gram-positive, non-motile, rod-shaped, non-spore-forming bacterium, designated CAAS 251T, was isolated from paper-mill effluent in Wuhan, China. The organism grew optimally at 40–42 °C and pH 9.0–10.0. major menaquinones were MK-7, MK-8 MK-9. predominant cellular fatty acids anteiso-C15 : 0 (34.78 %), anteiso-C17 (25.24 %) C16 (13.37 %). G+C content of the genomic DNA 65.5 mol%. phylogenetic analysis based on 16S rRNA gene sequences showed that strain 251T belongs to genus Nesterenkonia,...
BACKGROUND:Cancer cells survival depends on glucose metabolism and ATP. Inhibiting is a possible anticancer treatment. The phosphorylation of 2-deoxy-D-glucose (2-DG), which glycogen analogue, seriously affects the normal glycometabolism process, leading to ATP consumption. Studies showed that 2-DG could regulate RIP c-FLIP. This paper aimed investigate effect c-FLIP expression in HepG2 Hep3B cells, further illustrating mechanism regulating liver cancer cell apoptosis induced by TRAIL....
This study was designed to investigate the effect of long non-coding RNA (lncRNA) OIP5-AS1 on cell migration and invasion gallbladder cancer (GBC) its specific mechanism.The expressions lncRNA miR-143-3p in GBC lines (GBC-SD, SGC996 NOZ) epithelial cells (HGBE cells) were measured by qRT-PCR. After loss- gain-of-function experiments for GBC-SD cells, CCK-8 applied examine viability, scratch assay measure migration, transwell chamber inspect capacity. The interaction between predicted...
For the problems of large computational complexity and splitting attribute selection inclining to choose which has many values in ID3 algorithm, this paper presents an improved algorithm based on Information Entropy Attribute Weights. In it been combined with Taylor's theorem Similarity simplify calculation determine importance weights, amended information gain is accomplished as criteria. The results experiment comparison proved that can improve speed classification, significantly accuracy...
To investigate whether a virus constitutively expressing active Akt is useful to prevent cirrhosis induced by carbon tetrachloride (CCl4).Using cre-loxp technique, we created an Ad-myr-HA-Akt virus, in which labeled HA tag and its expression driven myr promoter. Further, through measuring enzyme levels histological structure, determined the efficacy of this inhibiting development CCl4 rats. Lastly, using western blotting, examined and/or phosphorylation status Akt, apoptotic mediators,...
To evaluate the in vitro differentiation of human amniotic epithelial cells (hAECs ) into hepatocyte-like cells.Combined approach dexamethasone, HGF, IGF and other cytokines were used to induce hAECs cells. The induction lasted 2 weeks. During induction, expression albumin ALB, CYP1A1, CYP1A2, IGFR, c-met key functional genes related liver as well transcription factors HNF3, HNF4 C/EBPa monitored by RT-PCR. Time dependent changes surface marker colony AFP CK18 analyzed cell flow...
Oilfield water injection is one of the important means to supplement energy formation and enhance oil recovery in process oilfield development. The level technology determines effect development also length life. Research on seepage law low-permeability reservoir basis technical development, key point analyze starting pressure gradient law. In previous studies, either static test or dynamic experimental value used, so error pseudo derived from too large, which makes people expand practical...
To determine the therapeutic effect of laparoscopic splenectomy, perisoph-agogastric devascularization, and endoscopic variceal ligation (EVL) on patients with portal hypertension.We randomly divided 105 into 3 groups: 40 had band (the group), 35 splenectomy devascularization laparotomy other 30 combination group). Blood samples were analyzed preoperatively postoperatively day 1,3,and 7,including alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL),and directed...
Human amniotic epithelial cells (hAECs) are a recently identified type of stem cell. Thanks to their ready availability and the lower risk teratoma formation, hAECs have been studied tested for variety human disease treatments tissue reconstruction efforts. This aim this study was establish stable tracking system further monitor in vivo after transplantation. were isolated from placentas patients who visited Hunan Province Maternity Child Care Hospitals between Jan 2008 2009. Using classic...
Objective: To investigate the clinical characteristics of patients with rheumatic diseases and abnormal liver function, as well determine proportion severity function abnormalities. Methods: Cross-sectional study. Data were collected from registered in Chinese Rheumatism Date Center 2011 to 2021. The analyzed this study rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), Sjogren syndrome (SS), ankylosing spondylitis (AS), gout. Patient data, including demographic [ such age, sex,...