A5053617741- Pain Mechanisms and Treatments
- Anesthesia and Pain Management
- Neuropeptides and Animal Physiology
- Anesthesia and Neurotoxicity Research
- Nerve injury and regeneration
- Anesthesia and Sedative Agents
- Cardiac, Anesthesia and Surgical Outcomes
- Pharmacological Effects of Natural Compounds
- Neuroscience and Neuropharmacology Research
- Cancer Treatment and Pharmacology
- MicroRNA in disease regulation
- Spine and Intervertebral Disc Pathology
- Cancer, Stress, Anesthesia, and Immune Response
- S100 Proteins and Annexins
- Cancer-related molecular mechanisms research
- Neuroinflammation and Neurodegeneration Mechanisms
- Airway Management and Intubation Techniques
- Hemodynamic Monitoring and Therapy
- Botulinum Toxin and Related Neurological Disorders
- Pediatric Pain Management Techniques
- Pain Management and Opioid Use
- Respiratory Support and Mechanisms
- Virus-based gene therapy research
- Cancer, Hypoxia, and Metabolism
- Nausea and vomiting management
Xiangya Hospital Central South University
2016-2025
Central South University
2016-2025
Hunan Children's Hospital
2011
Second Xiangya Hospital of Central South University
2011
The steroid hormone estrogen (17-β-estradiol, E2) provides neuroprotection against cerebral ischemic injury by activating receptors. novel receptor G protein-coupled 30 (GPR30) is highly expressed in the brain and acute stroke. However, underlying mechanisms remain unclear.In this study, ovariectomized female mice were subjected to middle artery occlusion (MCAO), E2, G1, ICI182780 administered immediately upon reperfusion. infarction volume, neurological scores, neuronal injuries examined....
Astrocytes possess different morphological characteristics depending on the cerebral region in which they are found. However, none of current astrocytic markers can label all subpopulations successfully. Thus, identifying appropriate marker for a specific scientific investigation is critical. Here, we compared distribution and protein expression three astrocyte markers: NDRG2, GFAP, S100 β , cortex, hippocampus, thalamus. NDRG2- -positive astrocytes were distributed more uniformly than...
Abstract In the treatment of uveal melanoma (UVM), histone deacetylase inhibitors (HDACi) have emerged as a promising epigenetic therapy. However, their clinical efficacy is hindered by suboptimal pharmacokinetics and strong self‐rescue tumor cells. To overcome these limitations, reactive oxygen species (ROS)‐responsive nanoparticles (NPs) are designed that encapsulate HDACi MS‐275 glutamine metabolism inhibitor V‐9302. Upon reaching microenvironment, NPs can disintegrate, thereby releasing...
Background: Neuropathic pain is detrimental to human health; however, its pathogenesis still remains largely unknown. Overexpression of pain-associated genes and increased nociceptive somato-sensitivity are well observed in neuropathic pain. The importance epigenetic mechanisms regulating the expression pro- or anti-nociceptive has been revealed by studies recently, we hypothesize that transcriptional coactivator histone acetyltransferase E1A binding protein p300 (p300), as a part gene...
Morphine tolerance is a challenging clinical problem that limits its application in pain treatment. Non-coding microRNAs (miRNAs) modulate gene expression post transcriptional manner, and their dysregulation causes various diseases. However, the significance of miRNAs morphine still poorly understood. In present study, we hypothesized microRNA-365 (miR-365) key functional small RNA reverses through regulation β-arrestin 2 rats. Here, microarray analysis quantitative real-time PCR showed...
Pain and depression are highly prevalent symptoms in cancer patients. They tend to occur simultaneously affect each other share biological pathways neurotransmitters. In this study, we investigated the roles of microglia hippocampus comorbidity bone pain depressive-like behaviors an animal model pain.
Abstract Bone cancer-induced pain (BCP) is a challenging clinical problem because traditional therapies are often only partially effective. Annexin A3 (ANXA3) highly expressed in microglia the spinal cord, and its expression upregulated during BCP. However, roles of microglial ANXA3 development maintenance BCP underlying molecular mechanisms remain unclear. This study was performed on male mice using metastatic lung model. Adeno-associated virus shANXA3 (AAV-shANXA3) injected intrathecally...
Morphine tolerance is a clinical challenge in pain management. Emerging evidence suggests that microRNA (miRNA) plays regulatory role the development of morphine tolerance. miR-219-5p (miR-219) targets calmodulin-dependent protein kinase II γ (CaMKIIγ) to activate central sensitization via N-methyl-D-aspartate (NMDA) receptor. Therefore, we hypothesized attenuates by targeting CaMKIIγ. We found expression was decreased significantly after chronic treatment. Overexpression lentivirus...
Background Neonatal exposure to isoflurane may induce long-term memory impairment in mice. Histone acetylation is an important form of chromatin modification that regulates the transcription genes required for formation. This study investigated whether neonatal exposure-induced neurocognitive related dysregulated histone hippocampus and it can be attenuated by deacetylase (HDAC) inhibitor trichostatin A (TSA). Methods C57BL/6 mice were exposed 0.75% three times (each 4 h) at postnatal days...
To the Editor: Chronic postoperative pain (CPSP) is one of most common complications after surgery. Depending on risk factors, as many 5–58% patients experience chronic surgery[1]. The mechanism CPSP complex and uncertain; current understanding suggests that persistent peripheral inflammation nociceptive hypersensitivity play crucial roles. Lidocaine a type sodium channel blocker widely used clinically for local anesthesia antiarrhythmia. Recently, studies have demonstrated lidocaine has...
Microglial inflammation is a hallmark of ischemic stroke. Annexin A3 (ANXA3) expressed in microglia and plays detrimental role However, the ANXA3 microglial after stroke unclear. In this study, an model was established mice via middle cerebral artery occlusion (MCAO). The adeno-associated virus shANXA3 (AAV-shANXA3) injected into ipsilateral cortex lesion, infarction volume, neurological score, neuronal injury were examined. Moreover, primary transfected with lentivirus (LV-shANXA3)...
Abstract Increased endothelial permeability and a dysregulated inflammatory response play key roles in organ damage sepsis. The role of annexin A3 (ANXA3) regulating inflammation during sepsis is explored using ANXA3 knockout mice primary human umbilical vein cells (HUVECs). absence exacerbated outcomes, including increased mortality, lung injury, leukocyte infiltration, vascular permeability. highly expressed its loss results the formation cytoskeletal stress fibers decrease expression...
In the spinal cord, protein kinase C isoform γ (PKCγ) plays an important role in development of central pain sensitization. However, there are currently no specific PKCγ inhibitors available. Therefore, aim present study was to assess modulation using a more selective experimental tool. Although small interfering RNAs have been used silence genes neurons, vivo delivery RNA interference (RNAi) remains major challenge, thus limiting its applications. Here we developed highly efficient method...
Background: Neuropathic pain is a complex state of chronic that usually accompanied by peripheral and central nervous system damage or dysfunction. Previous studies have indicated neuroinflammation in the spinal cord an important contributor to neuropathological behavioral abnormalities. A series early inflammatory markers, such as IL-1, TNF-α, IFN-γ, advanced high-mobility group box 1 (HMGB1), are involved neuroinflammation. Study Design: randomized, double blind, controlled animal trial....