A5047190174- Cancer-related gene regulation
- Hippo pathway signaling and YAP/TAZ
- TGF-β signaling in diseases
- Aldose Reductase and Taurine
- Cancer-related Molecular Pathways
- Chronic Lymphocytic Leukemia Research
- Ubiquitin and proteasome pathways
- Kruppel-like factors research
- Connective Tissue Growth Factor Research
- Cancer Mechanisms and Therapy
- Monoclonal and Polyclonal Antibodies Research
- Cancer, Hypoxia, and Metabolism
- Educational Innovations and Technology
- Curcumin's Biomedical Applications
- MicroRNA in disease regulation
- Wnt/β-catenin signaling in development and cancer
- Genomics and Phylogenetic Studies
- Venomous Animal Envenomation and Studies
- Immune cells in cancer
- Cancer, Lipids, and Metabolism
- Selenium in Biological Systems
- Cloud Computing and Resource Management
- Chemotherapy-induced organ toxicity mitigation
- Thallium and Germanium Studies
- vaccines and immunoinformatics approaches
Nanchang University
2012-2024
First Affiliated Hospital of Jiangxi Medical College
2024
Chinese University of Hong Kong
2019
Taipei Veterans General Hospital
1984
Taurine (Tau) has been shown to possess cancer therapeutic effect through induction of apoptosis, while the underlying molecular mechanism its anti-cancer is not well understood. PUMA (p53-upregulated modulator apoptosis) plays an important role in process apoptosis a variety human tumor cells both p53-dependent and -independent manners. However, whether involved Tau-induced studied. In present study, we treated colorectal HT-29 (mutant p53) LoVo (wild-type with different concentrations Tau,...
To investigate the effects of taurine on cell proliferation and apoptosis, human lung cancer A549 line xenograft tumors in nude mice were used. The apoptosis observed at time points 24, 48 72 h after treatment using an MTT assay to detect survival rate, flow cytometry apoptotic rate. Western blot analysis was performed examine levels p53 upregulated modulator (PUMA), BCL2, regulator (Bcl-2) BCL2-associated X, (Bax) cells. level PUMA, Bax Bcl-2 proteins mouse treated with and/or exogenous...
Adipocyte is the most predominant cell type in tumor microenvironment of breast cancer and plays a pivotal role progression, yet underlying mechanisms functional mediators remain elusive. We isolated primary preadipocytes from mammary fat pads human patients generated mature adipocytes cancer-associated (CAAs) vitro. The CAAs exhibited significantly different gene expression profiles as assessed by transcriptome sequencing. One highly expressed genes granulocyte colony-stimulating factor...
Abstract The rapid development and popularization of smart phones mobile internet has created a new social lifestyle, correspondingly prompts the transformation network teaching from desktop computer to teaching. This article compared pros cons Tsinghua Education Online WeChat official account (WOA) in fulfilling functions. We also described construction WOA platform with example WOA‐based biochemistry molecular biology. can establish nearly 75 menu catalogs 2,250 items, which is capable for...
TGF-β and Hippo signaling are two critical pathways engaged in cancer progression by regulating both oncogenes tumor suppressors, yet how the coordinately exert their functions development of hepatocellular carcinoma (HCC) remains elusive. In this study, we firstly conducted an integrated analysis public liver databases our experimental target genes, identifying CYR61 as a pivotal candidate gene relating to HCC development. The expression is downregulated clinical tissues cell lines than...
The aim of the present study was to observe effect and molecular mechanism taurine (Tau) on cell proliferation apoptosis human hepatocellular carcinoma (HHCC) HepG2 cells. HHCC cells were used as target cells, survival rate assessed using a multi-time-step method. p53 upregulated modulator (PUMA) gene transiently transfected by lipofection subsequently silenced with specific small interfering (si)RNA. detected flow cytometry, protein expression levels analyzed western blotting. Addition...
Feedback regulation plays a pivotal role in determining the intensity and duration of TGF-β signaling subsequently affecting pathophysiological roles TGF-β, including those liver malignancy.KLF2, member Krüppel-like factor (KLF) family transcription factors, has been implicated impeding hepatocellular carcinoma (HCC) development.However, underlying molecular mechanisms are not fully understood.In present study, we found that stimulates expression KLF2 gene several HCC cell lines.KLF2 protein...
Tea domain transcription factor 4 (TEAD4) plays a pivotal role in tissue development and homeostasis by interacting with Yes-associated protein (YAP) response to Hippo signaling inactivation. TEAD4 YAP can also cooperate transforming growth factor-β (TGF-β)-activated Smad proteins regulate gene transcription. Yet, it remains unclear whether YAP-independent TGF-β signaling. Here, we unveil novel tumor suppressive function of liver cancer via mitigating Ectopic inhibited TGF-β-induced signal...
Due to the therapeutic resistance of endocrine therapy and limited efficacy immune checkpoint inhibitors in estrogen receptor (ER)-positive breast cancer (BRCA), there is an urgent need develop novel prognostic markers understand regulation tumor microenvironment (TIME). As a matricellular protein, CYR61 has been shown either promote or suppress progression depending on types. However, how functions ER-positive BRCA remains elusive. In this study, we comprehensively analyzed expression based...
<b><i>Objectives:</i></b> The effect of p53 upregulated modulator apoptosis (PUMA) in hypoxia/reoxygenation-induced cardiomyocyte injuries rats was investigated. <b><i>Methods:</i></b> PUMA-targeting (si-PUMA) and scramble siRNAs were designed transfected into primarily rat cardiomyocytes vitro. <b><i>Results:</i></b> RT-PCR Western blot analysis showed that 50 nmol/l si-PUMA can specifically inhibit PUMA expression. MTT...
The aim of this study was to observe the impact mammalian sterile 20-like kinase 1-c-Jun N-terminal (MST1-JNK) signaling pathway on apoptosis in colorectal cancer (CRC) cells induced by Taurine (Tau). Caco-2 and SW620 transfected with p-enhanced green fluorescent protein (EGFP)-MST1 or short interfering RNA (siRNA)-MST1 were treated Tau for 48 h. Apoptosis detected flow cytometry, levels MST1 JNK western blotting. Compared control group, 80 mM could significantly induce CRC cells, apoptotic...
Mammalian STE20-like kinase 1 (Mst1) is the mammalian homologue of Drosophila Hippo, a major inhibitor cell proliferation in Drosophila. It ubiquitously encodes serine threonine that belongs to family protein kinases related yeast STE20, and involved proliferation, apoptosis, oncogenesis, organ growth. Recent studies have shown Mst1 has tumor-suppressor function, deletion or mutation reported be associated with tumorigenesis. To investigate effect overexpression on growth human liver cancer...
An integrated and projected-based laboratory course was described, integrating interconnected knowledge points biochemistry molecular biology techniques on a research project-based system. The program, which served as an essential extension of theoretical courses to practice, conducted with sophomore basic medical science who had completed the in biology. This engaged students learning "genetic manipulation" "recombinant DNA technology" understand target gene's role disease mechanics, thus...
The COVID-19 pandemic is a huge challenge to education systems. Most governments around the world have temporarily closed schools, universities, and colleges. At same time, teachers students are encouraged use online distance learning programs platforms as an alternative. In present study, we proposed series of innovative solutions in Medical Molecular Biology during China, including flipped classroom model, live streaming course, chat Apps, scientific papers on additional material. Our...
Aim: To investigate the effects of diallyl trisulfide (DT) on apoptosis cisplatin (DDP)-resistant human epithelial ovarian cancer SKOV-3 cells (SKOV-3/DDP), and role p53 upregulated modulator (PUMA). Methods: SKOV-3/DDP were randomly divided into control, DT, DPP DPP+DT groups, which treated with DT or combined DDP. All incubated for 48 h. rates assessed by flow cytometry. mRNA protein expression PUMA, Bax Bcl-2 was determined RT-PCR Western blot assays, respectively. Results: Compared...
Abnormal miR-761 expression involves in colon cancer and curcumin has a significant anti-tumor cell proliferation effect. In this study, we investigated whether targeting can affect cells. Control group (NC group), Mimics group, Mimics+curcumin were set up. GSK-3 β levels detected by RT-qPCR. Dual fluorescein report the relation between along with analysis of cloning assay apoptosis flow cytometry. Compared normal tissues, tissues was lower ( P <0.01), while level higher < 0.01)....
Abstract Splicing factor SF3B1 is frequently mutated in chronic lymphocytic leukemia (CLL) patients and has been suggested as a potential therapeutic target. In this study, we demonstrated that modulator sudemycin D6 (SD6) effectively inhibits cell growth induces apoptosis CLL cells. RNA sequencing analysis revealed significant increases global intron retention SD6-treated Pathway of the genes associated with increased B-cell receptor (BCR) PI3K signaling pathways were among most important...