A5022027469- Childhood Cancer Survivors' Quality of Life
- Epigenetics and DNA Methylation
- Family Support in Illness
- Acute Lymphoblastic Leukemia research
- Genetics and Neurodevelopmental Disorders
- Palliative Care and End-of-Life Issues
- Mitochondrial Function and Pathology
- Folate and B Vitamins Research
- Health, Environment, Cognitive Aging
- Genomic variations and chromosomal abnormalities
- RNA modifications and cancer
- Hormonal Regulation and Hypertension
- Genomics and Rare Diseases
- Metabolism and Genetic Disorders
- Endoplasmic Reticulum Stress and Disease
- Education Discipline and Inequality
- Pregnancy-related medical research
- Hematopoietic Stem Cell Transplantation
- Vitamin C and Antioxidants Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Preterm Birth and Chorioamnionitis
- Genetic Syndromes and Imprinting
- Advanced Biosensing Techniques and Applications
- Inflammatory mediators and NSAID effects
- Adrenal Hormones and Disorders
St. Jude Children's Research Hospital
2020-2025
Appalachian State University
2024
University of South Florida
2024
Princess Royal Maternity Hospital
2002
Ayrshire Central Hospital
2002
Birmingham Children's Hospital
1992
Queen Charlotte's and Chelsea Hospital
1974-1977
University of Fiji
1964
Abstract Monoclonal antibodies, My10 (HPCA-1) and major histocompatibility class II (HLA-DR), were used to enrich phenotype normal human marrow colony-forming unit: granulocyte-macrophage (CFU-GM), burst-forming erythroid (BFU-E), multipotential granulocyte-erythroid-macrophage-megakaryocyte (CFU-GEMM) progenitor cells. Nonadherent low density T lymphocyte-depleted cells sorted on a Coulter Epics 753 dye laser flow cytometry system with the use of Texas Red-labeled anti-My10 phycoerythrin...
Mounting evidence supports the occurrence of accelerating aging among long-term survivors childhood cancer. We aimed to investigate epigenetic age acceleration (EAA) in and evaluate associations between EAA, treatment exposures, health behaviors, chronic conditions (CHCs).Genome-wide methylation data were generated with Infinium EPIC BeadChip on blood-derived DNA from 2139 282 frequency matched controls St Jude Lifetime Cohort Study. EAAs estimated as residuals a linear regression (Levine's...
Abstract Sequence-based genetic testing identifies causative variants in ~ 50% of individuals with developmental and epileptic encephalopathies (DEEs). Aberrant changes DNA methylation are implicated various neurodevelopmental disorders but remain unstudied DEEs. We interrogate the diagnostic utility genome-wide array analysis on peripheral blood samples from 582 genetically unsolved identify rare differentially methylated regions (DMRs) explanatory episignatures to uncover candidate...
Importance Certain cancer therapies are risk factors for epigenetic age acceleration (EAA) among survivors of childhood cancer, and EAA is associated with chronic health conditions (CHCs). However, small numbers younger (aged <20 years) previously evaluated have limited the ability to calculate this group. Objective To evaluate change rate (EA) in compared older possible association early-onset obesity years), severity/burden CHCs, late mortality (>5 years from...
It is well-established that cancer treatment substantially increases the risk of long-term adverse health outcomes among childhood survivors. However, there limited research on underlying mechanisms. To elucidate pathophysiology and a possible causal pathway from exposures to cardiometabolic conditions, we conducted epigenome-wide association studies (EWAS) identify DNA methylation (DNAm) sites associated with examined whether treatment-associated DNAm mediate associations between specific...
Current research in epigenetic age acceleration (EAA) is limited to non-Hispanic White individuals. It imperative improve inclusivity by considering racial and ethnic minorities EAA research.
Background: Childhood cancer survivors experience accelerated aging, largely due to treatment toxicity. We investigated how aging biomarkers, which quantify and capture multifaceted mechanisms, differentially mediate treatment-related cardiotoxicity. Methods: Peripheral blood samples from in the St Jude Lifetime Cohort were profiled with EPIC BeadChip generate DNA methylation (DNAm) data, three established biomarkers subsequently derived: epigenetic age (EA, reflecting alterations),...
Abstract Background: Cancer treatments engender the risk of long-term cardiometabolic and cardiac conditions among survivors childhood cancer. However, underlying biological mechanisms remain unclear. This study aimed to identify DNA methylation (DNAm) CpG sites associated with factors (CMRFs) adverse outcomes, assess if these DNAm mediate associations between specific cancer outcomes. Methods: We included 3, 044 (median age at blood draw: 29.6 years) from St. Jude Lifetime Cohort....
Abstract Background: Social determinants of health (SDOH) are increasingly recognized as critical factors in shaping long-term adverse outcomes among childhood cancer survivors (survivors) because long-lasting toxicities from treatment may be exacerbated by social adversity. Our previous epigenetic study SDOH primarily focused on European ancestry, using measures such educational attainment, personal income, and the Area Deprivation Index. This aims to enhance understanding biological...
Abstract Background Childhood cancer survivors are at high risk for morbidity and mortality poor patient-reported outcomes, typically health-related quality of life (HRQOL). However, associations between DNA methylation–based aging biomarkers HRQOL have not been evaluated. Methods methylation was generated with Infinium EPIC BeadChip on blood-derived (median age blood draw = 34.5 years, range 18.5-66.6 years), assessed survey (mean 32.3 18.4-64.5 years) from 2206 in the St Jude Lifetime...
Social epigenomics is an emerging field in which social scientist collaborate with computational biologists, especially epigeneticists, to address the underlying pathway for biological embedding of life experiences. This study included long-term childhood cancer survivors enrolled St. Jude Lifetime Cohort. DNA methylation (DNAm) data were generated using Illumina EPIC BeadChip, and three determinants health (SDOH) factors assessed: self-reported educational attainment, personal income, area...
Abstract. Radioimmunoassay of sex steroids and prostaglandins was performed on plasma obtained from 10 uncomplicated primigravid subjects at a stated time the day varying stages gestation. Prostaglandin E 2 (PGE ), prostaglandin F 2α (PGF oestrone oestriol concentrations reached peak few weeks before term then declined. Progesterone, 17‐hydroxyprogesterone oestradiol increased during pregnancy particularly after 32 weeks. In two normal patients repeated blood samples were taken throughout...
Abstract Background DNA methylation (DNAm) plays an important role in lipid metabolism, however, no epigenome-wide association study (EWAS) of levels has been conducted among childhood cancer survivors. Here, we performed EWAS analysis with longitudinally collected blood data from survivors the St. Jude lifetime cohort study. Methods Among 2052 European ancestry (EA) and 370 African (AA), four types lipids, including high-density lipoprotein (HDL), low-density (LDL), total cholesterol (TC),...
Abstract We describe further development of the fumarylacetoacetase (FAA) assay for prenatal diagnosis tyrosinaemia type 1 using chorionic villus sampling (CVS). have established a reference range FAA activity in cultured cells and confirmed previously reported data on uncultured cells. This should allow confirmation results CVS, without need invasive procedures. report enzyme stability at −70°C, +4°C, room temperature, we shown no obvious difference with gestational age. analysed...
ABSTRACT Sequence-based genetic testing currently identifies causative variants in ∼50% of individuals with developmental and epileptic encephalopathies (DEEs). Aberrant changes DNA methylation are implicated various neurodevelopmental disorders but remain unstudied DEEs. Rare epigenetic variations (“epivariants”) can drive disease by modulating gene expression at single loci, whereas genome-wide result distinct “episignature” biomarkers for monogenic a growing number rare diseases. Here, we...
Abstract Background Many women of childbearing age with IBD require advanced therapies. While biologics are largely low risk during pregnancy, the novel small molecules Tofacitinib, Filgotinib, Upadacitinib and Ozanimod (TFUO) have shown concerning reproductive results in animal studies. Therefore, their use needs careful consideration. Methods We convened a RAND appropriateness panel 6 gastroenterologists, 2 nurses, 1 pharmacist 3 expert patients. Following literature review, 13 statements...
Abstract Background: Long-term survivors of childhood cancer experience high rates patient-reported symptoms and evidence for accelerated molecular aging. Both are established to increase the risk late-mortality (>5 years from diagnosis). We aim investigate if aging mediates association between all-cause late-mortality. Methods: DNA methylation (DNAm)-based biomarkers were derived DNAm Age Calculator, including epigenetic age acceleration (EAA) using multiple clocks (e.g., GrimAge...
Abstract Background: Childhood cancer survivors often reported poorer health-related quality-of-life (HRQOL) compared to age/sex-matched controls, indicating toxic stress resulting from high burden of treatment-related late effects. This study aimed investigate cross-sectional associations between HRQOL and epigenetic age acceleration (EAA) using several clocks as well other DNA methylation (DNAm)-based aging biomarkers. Methods: DNAm was generated the Infinium EPIC BeadChip version 1 with...
Abstract Background: Inflammaging, a chronic low-grade inflammation as individuals age, is measured by elevated levels of circulating markers (e.g., C-reactive protein [CRP] and interleukin-6 [IL-6]) associated with age-related health conditions (CHCs). We aim to evaluate epigenetic scores (EIS) for CRP IL-6 their associations cardiometabolic cardiac-based CHCs age acceleration (EAA) in childhood cancer survivors (CCS). Methods: Among 2,052 CCS (47.2% female; mean [SD] = 33.7 [9.3] years at...
Drawing data from the National Education Longitudinal Study, we examine whether adolescents’ perceived teacher and peer negative treatment (being put down by teachers in classroom being as a troublemaker peers) increase risk of school-based crime (property personal victimization). We also investigate conditions effects race (African American) ethnicity (Latino) on property victimization. found was significantly related to victimization even after controlling for other relevant factors....
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