A5101428143- Inflammatory Bowel Disease
- Microscopic Colitis
- Eosinophilic Esophagitis
- IL-33, ST2, and ILC Pathways
- Asthma and respiratory diseases
- Helicobacter pylori-related gastroenterology studies
- Gut microbiota and health
- Celiac Disease Research and Management
- Gastrointestinal motility and disorders
- Liver Diseases and Immunity
- S100 Proteins and Annexins
- Pregnancy and Medication Impact
- Eosinophilic Disorders and Syndromes
- Systemic Lupus Erythematosus Research
- Pharmaceutical studies and practices
- Allergic Rhinitis and Sensitization
- Social and Educational Sciences
- Infant Nutrition and Health
- Streptococcal Infections and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nerve Injury and Rehabilitation
- Sex and Gender in Healthcare
- Vagus Nerve Stimulation Research
- Genetic factors in colorectal cancer
- Acute Kidney Injury Research
Uppsala University
2007-2025
University of Gothenburg
2024
Uppsala University Hospital
1984-2018
University Hospital of Zurich
2011
Carnegie Mellon University
2011
Abstract Background and Aims The diagnostic prognostic properties of anti-integrin αvβ6 IgG autoantibodies in ulcerative colitis (UC) are poorly understood. We aimed to assess the performance examine their association with disease outcomes. Methods Serum samples from a Swedish inception cohort patients suspected inflammatory bowel (IBD, n=473) were analysed using an in-house fluorescence enzyme immunoassay based on EliA™technology. Findings validated Norwegian population-based (n=570)....
A 45 kDa-protein was purified from the granules of human neutrophils. The protein consists two apparently identical subunits. isoelectric point pH8.40, and molecular weight 45kDa (unreduced) or 24kDa (reduced). Treatment with Endoglucosidase F resulted in a reduction to 20 kDa, indicating presence N-linked carbohydrate. extinction coefficient E1%lcm = 13.76 at 280nm. 60 amino acid sequence revealed up 65% homology rat α2-microglobulin-related protein, which belongs lipocalin family....
Background Rescue therapy with infliximab (IFX) has been proven effective in a steroid-refractory attack of ulcerative colitis (UC). The long-term efficacy is not well described. Aim To present retrospective study IFX as rescue UC. Primary end points were colectomy-free survival at 3 and 12 months. Methods In this multicentre study, 211 adult patients hospitalised between 1999 2010 received 5 mg/kg due to steroid-refractory, moderate-to-severe Exclusion criteria duration current flare for...
Summary Background Faecal calprotectin, an established biomarker used to assess mucosal inflammation, has been shown correlate with endoscopic activity in inflammatory bowel disease ( IBD ). Longitudinal monitoring of faecal however, rarely employed beyond assessment therapy response and post hoc analyses clinical trials. Aim To study whether consecutive measurements calprotectin every third month are useful for patients remission. Methods Patients aged 18 years or older, a known diagnosis...
Abstract Improved biomarkers are needed for pediatric inflammatory bowel disease. Here we identify a diagnostic lipidomic signature disease by analyzing blood samples from discovery cohort of incident treatment-naïve patients and validating findings in an independent inception cohort. The comprising only lactosyl ceramide (d18:1/16:0) phosphatidylcholine (18:0p/22:6) improves the prediction compared with high-sensitivity C-reactive protein. Adding protein to does not improve its performance....
Background Data on the incidence of Eosinophilic gastrointestinal disease (EGID) distal to esophagus are scarce. This study aimed examine non-eosinophilic esophagitis (EoE) EGID in Sweden, as well its three entities; eosinophilic gastritis (EoG), enteritis (EoN), and colitis (EoC).
Purpose There is a need for diagnostic and prognostic biosignatures to improve long-term outcomes in inflammatory bowel disease (IBD). Here, we describe the establishment of Swedish Inception Cohort IBD (SIC-IBD) demonstrate its potential identification such signatures. Participants Patients aged ≥18 years with gastrointestinal symptoms who were referred gastroenterology unit due suspected at eight hospitals between November 2011 March 2021 eligible inclusion. Findings date In total, 367...
The mechanisms behind increased fecal calprotectin (FC) in healthy relatives of patients with inflammatory bowel disease (IBD) are unknown. Our aims were to explore if there is a subclinical inflammation neutrophil activity twin siblings discordant pairs IBD and assess the influence genetics this context.Nuclear factor kappa B (NF-κB) activity, based on myeloperoxidase (MPO) FC, analyzed compared controls. NF-κB MPO assessed by immunohistochemistry FC enzyme-linked immunosorbent assay.In...
Fecal calprotectin (FC) is a noninvasive tool for examining response to biologics in inflammatory bowel disease (IBD), but its performance relation other novel fecal markers of various cellular origins unknown.We performed prospective multicenter cohort study and included patients with active IBD who provided sample at initiation biological therapy. Levels FC, myeloperoxidase (MPO), human neutrophil lipocalin (HNL), eosinophil-derived neurotoxin (EDN) were analyzed related clinical remission...
Background: Non-invasive markers for predicting relapse would be a useful tool the management of patients with inflammatory bowel disease. Eosinophil granulocytes and their granule proteins eosinophil cationic protein (ECP) eosinophil-derived neurotoxin (EDN) have previously been shown to reflect disease activity in Crohn's ulcerative colitis.Aim: To examine capacity faecal ECP EDN predict colitis disease, compare these calprotectin.Methods: Patients (n = 49) 55) were followed prospectively...
Abstract Eosinophils have been linked to functional dyspepsia; however, less is known about their role in irritable bowel syndrome (IBS). This study tested the hypothesis of alterations levels fecal eosinophil-derived neurotoxin (F-EDN) and eosinophil density degranulation within colonic mucosa IBS patients compared with healthy controls (HC). Colonic biopsies were collected from 37 20 HC analyzed for numbers local cationic protein (ECP) by histologic procedures. Fecal samples F-EDN...
Abstract Background Better prognostic measures for ulcerative colitis (UC) could significantly advance patient care. While the capacity of circulating proteins in UC has been explored, role mucosal remains largely unknown. We examined protein markers patients with incident and evaluated their value. Methods Biopsies from macroscopically inflamed colonic/rectal mucosa adult Swedish inception cohort IBD (SIC IBD) were obtained at diagnosis UC. Patients followed prospectively, clinical data...
ABSTRACT Background Eosinophilic esophagitis (EoE) is a chronic, inflammatory disease of the esophagus. Chronic inflammation has been linked to cancer development. We aimed study potential association between EoE and later diagnosis. Methods In this nationwide population‐based cohort study, we identified 1580 individuals with diagnosed 1990–2017 through Sweden's 28 pathology departments. Up five general population reference were matched on age sex ( n = 7533). A Cox regression analysis...
Abstract Background and Aims: Seasonal allergy is an interesting model to study the pathophysiological mechanisms involved in allergic inflammation. However, experimental allergen exposure easier perform standardise. The primary aim of this was compare inflammatory responses high‐dose bronchial challenge natural during birch pollen season. second patients with rhinitis asthma, respectively both types exposure. Methods: Fifteen pollen‐allergic (seven asthma eight rhinitis) five healthy...
Radioimmunoassay of myoglobin (Mb) was performed in rat hind limb muscles after surgical denervation and during reinnervation following cryolesion the sciatic nerve. Muscles contralateral leg served as controls. After resection nerve, decreased Mb concentrations were noted on fourth day tibialis anterior, peroneus longus, extensor digitorum longus (EDL) muscles. Thereafter, levels increased up to last observation 32. The increases anterior EDL considerably more pronounced (305% 324%,...
Abstract Background Improved diagnostic biomarkers are an unmet clinical need for paediatric inflammatory bowel disease (IBD). We therefore aimed to identify a lipidomic signature of IBD in blood. Methods performed non-targeted liquid chromatography-time flight mass spectrometry-based lipidomics (UPLC-QTOFMS) analysis plasma samples from 211 treatment-naïve children with suspected (Table 1). Discovery cohort: the Uppsala Children’s Hospital cohort (n=94); Validation population-based IBSEN...
Abstract Background Mental health (MH) has been reported to be poorer among patients with inflammatory bowel disease (IBD) than general population. However, it is not known whether MH more driven by inflammation itself or related gastrointestinal (GI) symptoms. Also, the dynamics of following IBD diagnosis well understood. Methods In Swedish Inception Cohort (SIC-IBD), Crohn’s (CD), ulcerative colitis (UC) and unclassified (IBD-U) as symptomatic controls (SC) healthy (HC) filled in Patient...
Abstract Background We aimed to identify protein signatures predictive of a future diagnosis inflammatory bowel disease (IBD). Methods conducted case-control study, nested within large population-based cohorts with biorepositories. Samples were obtained from individuals who later in life diagnosed IBD (preclinical cases) and compared age sex-matched remained free during follow-up (controls). Using proximity extension assays (Olink, Uppsala), we measured 176 proteins. applied regularized...
Mastocytosis is a heterogeneous disorder characterized by abnormal mast cell accumulation, in which the clinical severity may be explained distinct molecular mechanisms. This study aimed to explore plasma protein biomarkers associated with systemic mastocytosis subtypes, as well cellular origin of identified proteins. Plasma samples from patients mastocytosis, including cutaneous (CM), indolent (ISM), and advanced (AdvSM), reference group polycythemia vera, were analyzed Proximity Extension...
Abstract Background The diagnosis of inflammatory bowel disease (IBD) is often delayed since existing biomarkers in blood are imprecise and faecal tests poorly accepted by patients. Therefore, we aimed to identify a diagnostic serum protein signature validate its performance utility. Methods Proximity extension immunoassay (PEA) methodology (Olink Proteomics, Uppsala, Sweden) was used measure 184 immune markers samples from adult patients with suspected IBD the Swedish inception cohort SIC...