A5109833888- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Adipose Tissue and Metabolism
- Cancer, Hypoxia, and Metabolism
- Chemokine receptors and signaling
- Congenital heart defects research
- Ion channel regulation and function
- Angiogenesis and VEGF in Cancer
- Cellular transport and secretion
- Cardiovascular Health and Risk Factors
- Adipokines, Inflammation, and Metabolic Diseases
- Mesenchymal stem cell research
- Cardiac electrophysiology and arrhythmias
- RNA Research and Splicing
- Lipid Membrane Structure and Behavior
- Cardiovascular Disease and Adiposity
- Atherosclerosis and Cardiovascular Diseases
- Neuroscience and Neural Engineering
- TGF-β signaling in diseases
- Cytokine Signaling Pathways and Interactions
- Metabolism, Diabetes, and Cancer
- Enzyme Production and Characterization
- Mobile Ad Hoc Networks
- Peroxisome Proliferator-Activated Receptors
- Circular RNAs in diseases
Peking University
2004-2024
Minzu University of China
2021-2023
State Ethnic Affairs Commission
2021-2023
Northwestern University
2011-2022
Renal Research Institute
2020-2021
Huazhong University of Science and Technology
2010-2018
Union Hospital
2010-2018
University of Alabama at Birmingham
2018
Indiana University – Purdue University Indianapolis
2018
Indiana University School of Medicine
2018
ABSTRACT Parvalbumin is a major allergen in fish. However, there currently no effective and safe way to remove this from In study, protease gene VSP2V‐280 of marine bacteria Virgibacillus sp. SP2 was cloned expressed. The enzyme showed maximum activity at 50°C pH 10.0. Ca 2+ Cu promoted the enzyme. good parvalbumin degradation efficiency Based on gel analysis, when 0.3 mg/mL incubated with (30 U/mL) containing 1 mM for 3 h, removal rate reached 97%. further used Ctenopharyngodon idella ,...
The mobilization of bone marrow (BM) progenitor cells (PCs) is largely governed by interactions between stromal cell-derived factor (SDF)-1 and CXC chemokine receptor (CXCR)4. Ischemic injury disrupts the SDF-1-CXCR4 interaction releases BM PCs into peripheral circulation, where mobilized are recruited to injured tissue contribute vessel growth. can also be pharmacological CXCR4 antagonist AMD3100, but other components signaling pathway unknown. c-kit, a membrane-bound tyrosine kinase for...
Circulating endothelial progenitor cells (circEPCs) of bone marrow (BM) origin contribute to postnatal neovascularization and represent a potential therapeutic target for ischemic disease. Statins are beneficial ischemia disease have been implicated increase via mechanisms independent lipid lowering. However, the effect on EPC function is not completely understood. Here we sought investigate effects Rosuvastatin (Ros) mobilization EPC-mediated during injury. In mouse model surgically-induced...
AimsThe E2F transcription factors are best characterized for their roles in cell-cycle regulation, cell growth, and death. Here we investigated the potential role of E2F1 cardiac neovascularization.
Abstract Hepatic gluconeogenesis is essential for glucose homeostasis and also a therapeutic target type 2 diabetes, but its mechanism incompletely understood. Here, we report that Sam68, an RNA-binding adaptor protein Src kinase substrate, novel regulator of hepatic gluconeogenesis. Both global deletions Sam68 significantly reduce blood levels the glucagon-induced expression gluconeogenic genes. Protein, not mRNA, CRTC2, crucial transcriptional gluconeogenesis, are >50% lower in...
Recent studies have identified a polymorphism in the endothelin-converting enzyme (ECE)-1b promoter (-338C/A) that is strongly associated with hypertension women. The located consensus binding sequence for E2F family of transcription factors. proteins are crucially involved cell-cycle regulation, but their roles cardiovascular function poorly understood. Here, we investigated potential role E2F2 blood pressure regulation.Tail-cuff measurements systolic and diastolic pressures were...
The effect of ginsenoside Rg 1 on vesicular neurotransmitter storage and release during exocytosis using single-vesicle electrochemistry.
The quantitative analysis of vesicular neurotransmitters in neurons situ is paramount for investigating neurodegenerative disorders, such as Alzheimer's disease and Parkinson's (PD). Unfortunately, a direct approach monitoring neurotransmitter chemistry single vesicles fresh brain tissue has remained inaccessible so far. Here, we introduce an innovative platform single-vesicle electrochemistry (SVE) tissue, enabling the quantification at level both soma varicosity. Utilizing this...
Insufficient neovascularization, characterized by poor endothelial cell (EC) growth, contributes to the pathogenesis of ischemic heart disease and limits cardiac tissue preservation regeneration. The E2F family transcription factors are critical regulators genes responsible for cell-cycle progression growth; however, specific roles individual E2Fs in ECs not well understood. Here we investigated E2F2 E2F3 EC angiogenesis, their functional impact on myocardial infarction (MI). An...
The majority of current cardiovascular cell therapy trials use bone marrow progenitor cells (BM PCs) and achieve only modest efficacy; the limited potential these to differentiate into endothelial-lineage is one major barriers success this promising therapy. We have previously reported that E2F transcription factor 1 (E2F1) a repressor revascularization after ischemic injury.We sought define role E2F1 in regulation BM PC function.Ablation (E2F1 deficient) mouse PCs increases oxidative...
Angiotensin (Ang) II is the major bioactive peptide of renin–angiotensin system (RAS); it contributes to pathogenesis hypertension by inducing vascular contraction and adverse remodeling, thus elevated peripheral resistance. Ang also activates Src family kinases (SFK) in system, which has been implicated cell proliferation migration. However, role SFK II-induced largely unknown. In this study, we found that administration a inhibitor SU6656 markedly lowered level systemic BP II-treated mice,...
In this study, we investigated the effects of Nano-SiO2, ultraviolet (UV) irradiation, and hydrogen peroxide on growth four marine aquatic pathogens as well their polysaccharide, biofilm formation, protein, DNA. The results indicated that among all concentrations, 100 μg/L Nano-SiO2 demonstrated most inhibition Edwardsiella tarda, Vibrio anguillarum, parahaemolyticus. Ultraviolet irradiation for 3 min had obvious inhibitory effect E. but neither nor promoting other three strains. High...
Obesity is associated with insulin resistance and type 2 diabetes; molecular mechanisms that promote energy expenditure can be utilized for effective therapy. Src-associated in mitosis of 68 kDa ( Sam68 ) potentially significant, because knockout (KO) leads to markedly reduced adiposity. In the present study, we sought determine mechanism by which regulates adiposity homeostasis. We first found KO mice have a significantly body weight as compared controls, difference explained entirely...
The goal of this work was to investigate the role t-tubule (TT) remodeling in abnormal Ca2+ cycling ventricular myocytes failing dog hearts. Heart failure (HF) induced using rapid right pacing. Extensive changes echocardiographic parameters, including left and dilation systolic dysfunction, diastolic elevated filling pressures, cardiac mechanics, indicated that severe HF developed. TT loss extensive when measured as density total cell volume, derived from three-dimensional confocal image...
We have identified a novel form of abnormal Ca2+ wave activity in normal and failing dog atrial myocytes which occurs during the action potential (AP) is absent diastole. The goal this study was to determine if triggered waves affect cellular electrophysiological properties.Simultaneous recordings intracellular APs allowed measurements maximum diastolic AP duration calcium (TCWs) isolated myocytes. Computer simulations then explored behavior arising from TCWs at tissue scale.At 3.3 5 Hz,...
BIN1 (amphyphysin-II) is a structural protein involved in T-tubule (TT) formation and phosphatidylinositol-4,5-bisphosphate (PIP2) responsible for localization of to sarcolemma. The goal this study was determine if PIP2-mediated targeting sarcolemma compromised during the development heart failure (HF) TT remodeling. Immunohistochemistry showed co-localization BIN1, Cav1.2, PIP2, phospholipase-Cβ1 (PLCβ1) TTs normal rat human ventricular myocytes. PIP2 levels were reduced spontaneously...
Genetic deletion of Src associated in mitosis 68kDa (Sam68), a pleiotropic adaptor protein prevents high-fat diet–induced weight gain and insulin resistance. To clarify the role Sam68 energy metabolism adult stage, we generated an inducible knockout mice. Knockout was induced at age 7-10 weeks, then examined metabolic profiles mice gained less body over time 34 or 36 weeks old, had smaller fat mass without changes food intake absorption efficiency. Deletion elevated thermogenesis, increased...
Abstract Chemotherapy with cisplatin induces side effects such as memory loss, confusion of thinking, and difficulties multi‐tasking. However, the mechanism inducing nervous dysfunction is still unknown. Herein, we examine whether how regulates release neurotransmitter during exocytosis in single chromaffin cells using cell amperometry. The results show that reduces amount transmitter released by reducing duration exocytotic events, including opening closing time fusion pore. Furthermore,...