A5103225532- Infant Nutrition and Health
- Neuroscience and Neuropharmacology Research
- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
- Sphingolipid Metabolism and Signaling
- Lipid Membrane Structure and Behavior
- Metabolomics and Mass Spectrometry Studies
- Glycosylation and Glycoproteins Research
- Natural Compounds in Disease Treatment
- Cancer-related gene regulation
- Stress Responses and Cortisol
- Reproductive Biology and Fertility
- Neurotransmitter Receptor Influence on Behavior
- Tissue Engineering and Regenerative Medicine
- Muscle and Compartmental Disorders
- Extracellular vesicles in disease
- COVID-19 diagnosis using AI
- Cell death mechanisms and regulation
- Mesenchymal stem cell research
- Enzyme Production and Characterization
- Histone Deacetylase Inhibitors Research
- Medical Research and Treatments
- Tryptophan and brain disorders
- Phytoestrogen effects and research
- SARS-CoV-2 detection and testing
Sichuan University
2017-2023
Zhejiang Chinese Medical University
2023
Hangzhou Hospital of Traditional Chinese Medicine
2010-2023
Zhejiang Center for Disease Control and Prevention
2022-2023
State Key Laboratory of Biotherapy
2017-2022
Chengdu Center for Disease Control and Prevention
2021
State Key Laboratory of Microbial Technology
2015-2020
China Three Gorges University
2020
Yichang Central People's Hospital
2020
Shandong University
2015-2020
Abstract Metabolic interaction between cancer-associated fibroblasts (CAFs) and colorectal cancer (CRC) cells plays a major role in CRC progression. However, little is known about lipid alternations CAFs how these metabolic reprogramming affect metastasis. Here, we uncover conditioned medium (CM) promote the migration of compared with normal CM. undergo lipidomic reprogramming, accumulate more fatty acids phospholipids. CM after protein deprivation still increase migration, which suggests...
Introduction: Serine hydroxymethyltransferase 2 (SHMT2) plays a critical role in serine-glycine metabolism to drive cancer cell proliferation. However, the nonmetabolic function of SHMT2 tumorigenesis, especially human colorectal (CRC) progression, remains largely unclear. Methods: expression CRC cells was identified by western blot and immunofluorescence assay. The proliferation, migration, invasion after knockdown or overexpression were explored through vitro vivo assays....
α‐ l ‐Fucosidases are key enzymes for the degradation of intestinal glycans by gut microbes. In this work, three putative ‐fucosidases (Afc1, Afc2, and Afc3) genes from Clostridium perfringens ATCC 13124 were cloned expressed in Escherichia coli . Afc1 had ‐fucosidase domain glycoside hydrolase (GH) 29 family but showed no enzyme activity toward all substrates examined. The acid/base residue Afc1, Ser205, was replaced a glutamic acid which is conserved GH29‐B ‐fucosidases. However, mutant...
Proteinuria is an independent risk factor for the progression of diabetic nephropathy (DN) and imbalance in podocyte function aggravates proteinuria. Celastrol primary active ingredient T. wilfordii, effective treating DN renal injury; however, mechanisms underlying its effect are unclear. We explored how celastrol prevents damage using vivo vitro experiments. randomly divided 24 male C57BLKS/J mice into three groups: db/m (n = 8), db/db groups (db/db + celastrol, 1 mg/kg/d, gavage...
Biallelic genetic variants in N -acetylneuraminic acid synthase ( NANS ), a critical enzyme endogenous sialic biosynthesis, are clinically associated with neurodevelopmental disorders. However, the mechanism underlying neuropathological consequences has remained elusive. Here, we found that mutation resulted absence of both and protein polysialylation cortical organoids notably reduced proliferation expansion neural progenitors. dysregulated migration differentiation, disturbed synapse...
The aim of this study was to investigate the renoprotective effects exosomes derived from rat bone marrow mesenchymal stem cells (rBMSCs) in a model 5/6 nephrectomy (Nx)-induced chronic kidney disease (CKD).A Nx-induced CKD established using conventional method. rBMSC-derived were isolated ultracentrifugation and characterized. injected into Nx rats through caudal vein. After 12 weeks, 24 h proteinuria, serum creatinine (SCr), blood urea nitrogen (BUN) levels evaluated, renal pathology...
Focal segmental glomerulosclerosis (FSGS) is the most common glomerular etiology of end-stage kidney disease (ESKD). Increasing evidence has indicated reparative potential mesenchymal stem cells (MSCs) in damaged diseased kidneys. However, effect bone marrow (BMSCs) on FSGS progression remains unclear. This study aimed to investigate protective effects BMSCs progression. A rat model was generated via unilateral nephrectomy plus adriamycin injection. Rat were isolated and characterized basis...
Renal injury is an important factor in the development of chronic kidney diseases that pathologically manifested as renal fibrosis and podocyte damage. In disease state, fibroblasts lead to high expression levels α-smooth muscle actin (α-SMA), while podocytes undergo epithelial–mesenchymal transition, leading proteinuria. Celastrol, a bioactive compound medicinal plant Tripterygium wilfordii, was found delay progression early diabetic nephropathy attenuate mice with unilateral ureteral...
Lipid metabolic alterations are associated with cancer progression. Lysine‑specific demethylase 1 (LSD1) plays a crucial role in and has become promising target for therapy. However, the effect of LSD1 on lipid metabolism remains unclear. In present study, we used LC‑MS/MS‑based lipidomics approach to investigate impact cell using ZY0511, specific inhibitor developed by our group as probe. ZY0511 profoundly modified human colorectal cervical metabolism. A total 256 differential metabolites...
Background: Cancer associated fibroblasts (CAFs) undergo a metabolic reprogramming to support tumor metastasis. However, lipid alterations of CAFs and underlying mechanism in colorectal cancer (CRC) progression remain unclear.Methods: Human normal (NFs) are isolated from fresh CRC samples. The proliferation migration cells detected by MTT assay, Wound-healing Transwell qRT-PCR, Western bolt Immunofluorescent (IF). profiles CAFs, NFs their CM UPLC-Q-TOF/MS based on lipidomic assay. verified...
Cefepime exhibits a broad spectrum of antimicrobial activity and thus is widely used treatment for severe bacterial infections. Adverse effects on the central nervous system (CNS) have been reported in patients treated with cefepime. Current explanation adverse neurobehavioral effect cefepime mainly attributed to its ability cross blood-brain barrier competitively bind GABAergic receptor; however, underlying mechanism largely unknown. In this study, mice were intraperitoneally administered...
N-acetylneuraminic acid synthase (NANS), the gene encoding for (NeuNAc; sialic acid), is closely associated with infantile-onset severe developmental delay and skeletal dysplasia. However, role involved mechanisms of NANS functioning have not been fully understood to date. Here, we generated a homozygous NANS-knockout human induced pluripotent stem cell (iPSC) line, NCCSEDi001-A-1, via CRISPR/Cas9-based editing method. The NCCSEDi001-A-1 line does express protein, but maintains normal...