A5100390021- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Angiogenesis and VEGF in Cancer
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Galectins and Cancer Biology
- Natural Compounds in Disease Treatment
- Multiple Myeloma Research and Treatments
- HER2/EGFR in Cancer Research
- Cancer Research and Treatments
- Peptidase Inhibition and Analysis
- Cancer Cells and Metastasis
- Immune cells in cancer
- Cell Adhesion Molecules Research
- Cancer, Hypoxia, and Metabolism
- Natural product bioactivities and synthesis
- Traditional Chinese Medicine Analysis
- Virus-based gene therapy research
- Parasites and Host Interactions
- Colorectal Cancer Treatments and Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Remote Sensing and LiDAR Applications
Nanjing Medical University
2024
Yantai University
2023
China Pharmaceutical University
2013-2022
First Affiliated Hospital of Harbin Medical University
2022
Harbin Medical University
2022
Second Affiliated Hospital of Zhengzhou University
2020
State Key Laboratory of Natural Medicine
2017-2019
Zhujiang Hospital
2019
Southern Medical University
2019
Second Affiliated Hospital of Nanjing Medical University
2012-2018
Tumor-associated macrophages (TAM) are prominent components of tumor microenvironment (TME) and capable promoting cancer progression. However, the mechanisms for formation M2-like TAMs remain enigmatic. Here, we show that lactate is a pivotal oncometabolite in TME drives macrophage M2-polarization to promote breast proliferation, migration, angiogenesis. In addition, identified activation ERK/STAT3, major signaling molecules pathway, deepens our molecular understanding how educates TAMs....
Biodegradable cationic nanoparticles have promising application as a gene delivery system. In this article, heparin−polyethyleneimine (HPEI) nanogels were prepared, and these developed nonviral vector. The transfection efficiency of HPEI was comparable with that PEI25K, while the cytotoxicity lower than PEI2K much PEI25K in vitro. These also had better blood compatibility PEI25K. After intravenous administration, degraded, degradation products excreted through urine. plasmid expressing...
Abstract Phage peptide display is a powerful technique for discovery of various target-specific ligands. However, target-unrelated peptides can often be obtained and cause ambiguous results. Peptide PB-TUP has been isolated repeatedly in our laboratory on different targets we conducted research phage to investigate their binding properties rate propagation. ELISA recovery assay demonstrated that had significant superior affinity polystyrene solid surface compared with control clones. In this...
The expression level of folate receptor alpha (FRα) is located highly rate in ovarian cancer though it remained absent normal tissues. This tumor restricted profile makes FRα a promising target for therapy and diagnosis. In this research we report binding peptide C7(Met-His-Thr-Ala-Pro-Gly-Trp-Gly-Tyr-Arg-Leu-Ser) discovered by phage display showed specific to expressing cells cell ELISA flow cytometry. Tumor targeting ability C7 was proved vivo both homing experiment fluorescence imaging....
B-cell maturation antigen (BCMA) is a highly plasma cell-selective protein expressed on malignant cells of patients with multiple myeloma (MM), and it defined therapeutic target. Major histocompatibility complex class I-related chain A (MICA) frequently in lymphoproliferative malignancies including MM. MICA activates natural killer (NK) costimulates T by interaction its immunoreceptor NK cell receptor G2D (NKG2D). Nonetheless, during full-blown MM, tumor promote efficient shedding, which...
Combination of antiangiogenesis and chemotherapy holds vast promise for effective inhibition tumor proliferation invasion. Herein, a multifunctional self-assembled nanosystem consisting amphiphilic c(RGDyK)-functionalized low-molecular-weight heparin-gambogic acid conjugate (cRHG) is developed, using c(RGDyK) peptide as αv β3 integrin targeting moiety to realize double-targeted delivery both cells angiogenic vasculature. cRHG with markedly decreased anticoagulant activity can self-assemble...
Background Schistosomiasis remains a major public health problem in China. The endemic areas are located the lake and marshland regions of southern China, particularly along middle low reach Yangtze River. Spatial analytical techniques often used epidemiology to identify spatial clusters disease regions. This study assesses distribution schistosomiasis explores high-risk Hubei Province, China provide guidance on control Methods In this study, autocorrelation methodologies, including global...
Cluster of differentiation 24 (CD24) is a specific surface marker involved in the tumorigenesis and progression hepatocellular carcinoma (HCC). However, all reported anti-CD24 antibodies are murine ones with inevitable immunogenicity. To address this, method using both molecular structure docking-based complementarity determining region (CDR) grafting was employed for humanization. After xenogeneic CDR into human antibody, three types canonical residues (in VL/VH interface core, loop...
The IL-13Rα1 signalling pathway and M2 macrophages play crucial roles in schistosome egg-induced hepatic fibrosis via the expression of pro-fibrotic molecules. This study aims to investigate inhibitory effect mechanism action corilagin on vitro vivo. mRNA protein IL-13Rα1, PPARγ, KLF4, SOCS1, STAT6, p-STAT6 TGF-β was measured with treatment after IL-13 stimulation vivo effectively killing adult schistosomes schistosome-infected mice. Histological analysis liver tissue assessed for degree...
Triptolide, a major active component of Tripterygium wilfordii Hook F (TWHF), is known to have multiple pharmacological activities. However, studies also shown that triptolide highly toxic the reproductive system by disrupting normal androgen and estrogen signaling. In present study, we investigated effect (5, 10, or 20 nM for 24 h) on estradiol production rat granulosa cells. Triptolide inhibited basal human chorionic gonadotropin (HCG)- 8-bromo-cAMP-stimulated as revealed RIA assay....
Heparin, a potential blood anti-coagulant, is also known for its binding ability to several angiogenic factors through electrostatic interactions due polyanionic character. However, the clinical application of heparin cancer treatment limited by drawbacks, such as unsatisfactory therapeutic effects and severe anticoagulant activity that could induce hemorrhaging. Herein, low molecular weight (LMWH) was conjugated ursolic acid (UA), which an angiogenesis inhibitor, amine group aminoethyl-UA...
Multiple myeloma (MM), a plasma cell malignancy, is the second most prevalent hematologic malignancy in US. Although much effort has been made trying to understand etiology and complexities of this disease with hope developing effective therapies, MM remains incurable at time. Because their antiproliferative proapoptotic activities, interferons (IFNs) have used treat various malignancies, including MM. some success observed, inherent toxicities IFNs limit efficacy. To address problem, we...
Interferon-α (IFNα) has multiple antitumor effects including direct toxicity and the ability to potently stimulate both innate adaptive immunity. However, its clinical applications in treatment of malignancies have been limited because short half-life serious adverse reactions when attempting deliver therapeutically effective doses. To address these issues, we fused IFNα2a anti-vascular endothelial growth factor receptor 2 (VEGFR2) antibody JZA00 with goal targeting it tumor microenvironment...
Transplantation of encapsulated living cells is a promising approach for the treatment wide variety diseases. Bioactive glass (bioglass) can be used drug delivery and other regenerative medicine applications. First all, we established scenario bioglass-incorporated alginate encapsulation. Then studied expansion murine embryonic stem (mESCs) in bioreactors with exposure to 70s bioglass. Finally, an integrated osteogenic differentiation mESCs presence bioglass was investigated. The...
Vascular endothelial growth factor receptor 2 (VEGFR-2) plays a critical role in tumor angiogenesis. None therapeutic antibodies targeting VEGFR-2 are available clinical use. Herein, we describe the screening of new single-chain antibody fragment (scFv) extracellular domain 3 human (kinase insert domain-containing [KDR]3) from Griffin phage display scFv library. A comprehensive sequence analysis was performed to assign framework and complementary-determining regions. The exerted particular...
Abstract To prevent protein aggregation, some proteins are usually expressed as fusion from which target can be released by proteolytic or chemical reagents. In this report, small ubiquitin‐related modifier (SUMO) linked with a hexa‐histidine tag was used partner for the antitumor‐analgesic peptide venom of Buthus martensii (Karsch) scorpion (AGAP). The optimal expression level soluble protein, SUMO‐AGAP, up to 40% total cellular protein. purified Ni‐NTA affinity chromatography and cleaved...