A5101811085- MicroRNA in disease regulation
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced biosensing and bioanalysis techniques
- Mesenchymal stem cell research
- Nanoparticle-Based Drug Delivery
- Glioma Diagnosis and Treatment
- Angiogenesis and VEGF in Cancer
- Redox biology and oxidative stress
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Graphene and Nanomaterials Applications
- Lanthanide and Transition Metal Complexes
- Cancer Cells and Metastasis
- interferon and immune responses
- Dendrimers and Hyperbranched Polymers
- Immune cells in cancer
- Bacterial biofilms and quorum sensing
- Corrosion Behavior and Inhibition
- Environmental Changes in China
- Magnesium Alloys: Properties and Applications
Binzhou Medical University
2023-2025
Binzhou University
2023-2025
Agro-Environmental Protection Institute
2024
Ministry of Agriculture and Rural Affairs
2024
Second Affiliated Hospital of Guangzhou Medical University
2019-2024
Guangzhou Medical University
2019-2024
Southeast University
2020
Zhongda Hospital Southeast University
2016-2019
Chinese Academy of Sciences
2014-2015
Hefei Institutes of Physical Science
2014-2015
Our previous study demonstrated the potential therapeutic role of human neural stem cell-derived exosomes (hNSC-Exo) in ischemic stroke. Here, we loaded brain-derived neurotrophic factor (BDNF) into derived from NSCs to construct engineered (BDNF-hNSC-Exo) and compared their effects with those hNSC-Exo on stroke both vitro vivo. In a model H2O2-induced oxidative stress NSCs, BDNF-hNSC-Exo markedly enhanced cell survival. rat middle cerebral artery occlusion model, not only inhibited...
Glioblastoma multiforme (GBM) is the most lethal brain malignancy which involves multi‐gene abnormality. Unfortunately, effective therapy against GBM remains lacking. Previously, we found that NRP‐1 and its downstream NRP‐1/GIPC1 pathway played an important role in GBM. In our study, further investigated upstream signaling of to understand how it regulated. First, identified hsa‐miR‐124‐3p was miRNA differentially expressed normal tissues by high‐throughput sequencing. Then, dual luciferase...
Transplanted neural stem cells promote tissue regeneration and functional recovery primarily by releasing paracrine factors. Exosomes act as important secreted molecules to deliver therapeutic agents involved in cellular functions. Here, we focused on the role of exosomes (hNSC-Exo) derived from human (hNSCs). We utilized pro-inflammatory factor interferon gamma (IFN-γ) induce generation altered (IFN-γ-hNSC-Exo), compared their roles with those hNSC-Exo explored potential mechanism....
Currently, exosomes (EXOs) are being explored as novel drug delivery carriers with greater advantages, including crossing the blood‑brain‑barrier and loading drugs. The present study utilized EXOs derived from neural stem cells (NSCs) for of molecular drugs to treat gliomas. <em>miR‑124‑3p</em> was selected according previous studies by authors, effects miR‑124‑3p glioma NSC‑EXOs <em>in vitro</em> vivo</em> were evaluated. It found that successfully delivered into cells, loaded significantly...
Mild hypothermia has been proven to inhibit microglia activation after TBI. Exosomal microRNA derived from played a critical role in promoting neurite outgrowth and synapse recovery. Here, we aimed investigate the of microRNAs microglial exosomes treatment on neuronal regeneration For vitro study, stretch-injured neurons were co-cultured with exosomes. vivo C57BL/6 mice under controlled cortical impact injected The results showed that MG-LPS-EXOHT increased number dendrite branches total...
A pH-responsive nanoplatform, hydroxylated mesoporous nanosilica (HMNS) coated by polyethylenimine (PEI) coupled with gadolinium and folic acid (FA) (Gd-FA-Si), was designed to deliver anticancer drug targeting promote contrast effect for tumor cells using magnetic resonance (MR) spectrometer. Doxorubicin (DOX) chosen as the loaded into nanopores of HMNS, then its release in simulated body fluid could be controlled through adjusting pH. This nanoplatform significantly enhance MR effect,...
An efficient enzyme-responsive controlled release carrier system was successfully fabricated using single-stranded DNA encapsulated functional mesoporous silica nanoparticles. Mesoporous nanoparticles were initially through hydrolysis of tetraethyl orthosilicate (TEOS) in cetyltrimethylammonium chloride (CTAC) solution, and the surface could be with DNA. This nanomaterial has excellent bioactivity its hydrolysate cannot cause damage to normal cell, thus biocompatibility is improved. In...
Abstract Our objective was to determine the molecular mechanisms by which lncRNA HOXA‐AS3 regulates biological behaviour of glioblastoma multiforme (GBM). We used an microarray assay identify GBM‐related expression profiles. Qrt‐PCR survey levels long non‐coding RNA (lncRNA) and target gene. Dual‐luciferase reporter assays were investigate interaction HOXA‐AS3, gene miRNA. Western blot analysis examine USP3 epithelial‐mesenchymal transition (EMT) genes. The MTT assay, transwell wound healing...
Stroke recovery is associated with neural stem cell (NSC) development and neurovascular unit reconstruction. The exosome, as an important intercellular player in communication, mediates neuro-restorative events by transferring exosomal protein RNA cargoes. In this study, we explored the role of microRNAs (miRNAs) human NSCs (hNSCs), analyzed expression profiles miRNAs hNSC-derived hypoxic preconditioning exosomes help next generation sequencing (NGS). results demonstrated that a certain...
Ischemic stroke, being a prominent contributor to global disability and mortality, lacks an efficacious therapeutic approach in current clinical settings. Neural stem cells (NSCs) are type of cell that only found inside the nervous system. These can differentiate into various kinds cells, potentially regenerating or restoring neural networks within areas brain have been destroyed. This review begins by providing introduction existing approaches for ischemic followed examination promise...
Glioma occurs due to multi-gene abnormalities. Neuropilin-1 (NRP-1), as a transmembrane protein, involves in glioma proliferation, invasion, and migration, well tumor angiogenesis. The cytoplasmic GAIP/RGS19-interacting protein (GIPC1), could regulate the clathrin-vesicles trafficking recycling. Here, we show that NRP-1 co-localizes co-immunoprecipitates with GIPC1, C-terminal SEA-COOH motif of interacts specially named from three proteins: PSD-95 (a 95 kDa involved signaling at...
As we enter the post-antibiotic era, rise of antibiotic-resistant pathogenic bacteria is becoming a serious threat to public health. This problem further complicated by biofilms, for which current treatment options are limited.To tackle this challenge, propose novel approach that involves use photodynamic cationic pH-sensitive liposomes loaded with ultra-small copper oxide (Ce6@Lipo/UCONs) effectively eliminate drug-resistant and eradicate biofilms while minimizing safety concerns risk...
Ischemic stroke represents the leading cause of adult neurological disability, with no effective therapeutic strategy. Stem cell transplantation promises a new promising for treating stroke, through replacement and cytokine paracrine. However, due to effect hostile immune microenvironment, survival differentiation stem cells are limited in vivo. Furthermore, delayed inflammatory response induced secondary injury. IFN-γ as pro-inflammatory has potential protect population during response,...
Inflammatory response generated by ischemic stroke commonly affects functional or structural recovery. The aim of this study was to examine the IFN-γ caused inflammatory effects on NSCs in vitro and vivo. We found that did not affect proliferation but increased SOD2 level oxidative stress culturing. High dose (500 ng) injection aggravated inflammation cerebral model alter repairing functions based treatment, including NSCs-IFN-γ combined significantly improved microenvironment decreasing...
So far many people still believe that there is a lack of effective drugs or strategies to restore the dysfunction and/or damaged structure refractory neurological diseases. However, neurorestorative treatments are being tried change state. Transplanted neural progenitor/precursor cells (NPCs) as novel therapy strategy played an important role in promoting function restoration. Evidence demonstrated positive effects grafted NPCs were mediated mainly by releasing paracrine factors, especially...