A5040750131- Cutaneous Melanoma Detection and Management
- Immunotherapy and Immune Responses
- Melanoma and MAPK Pathways
- Viral Infectious Diseases and Gene Expression in Insects
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Microbial infections and disease research
- Mycobacterium research and diagnosis
- melanin and skin pigmentation
- Immune Response and Inflammation
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer Research and Treatments
- Reproductive System and Pregnancy
- Hematopoietic Stem Cell Transplantation
- Veterinary Oncology Research
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- RNA regulation and disease
- Cell Image Analysis Techniques
- Telomeres, Telomerase, and Senescence
- Heat shock proteins research
Génétique Animale et Biologie Intégrative
2009-2024
Institut de Radiobiologie Cellulaire et Moléculaire
2007-2024
Département Génétique Animale
2001-2024
Université Paris-Saclay
2015-2023
AgroParisTech
2011-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2023
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2003-2020
Direction de la Recherche Fondamentale
2018
CEA Grenoble
2017
CEA Cadarache
2017
Abstract Tumors are complex tissues composed of neoplastic cells, soluble and insoluble matrix components stromal cells. Here we report that in melanoma, turn‐over type I collagen (Col(I)), the predominant protein dermal stroma affects melanoma progression. Fibroblasts juxtaposed to cell nests within papillary dermis display high levels Col(I) mRNA expression. These enveloped by fibers. In contrast, melanoma‐associated fibroblasts reticular express at a level is comparable its expression...
Pattern recognition receptors (PRRs) play a critical role in the detection of microorganisms and induction inflammatory immune responses. Using PCR Western-blot analysis, this study investigated differential expression intestine 14 PRRs nine associated cytokines. Thirty-two pigs were used to determine these markers (1) along proximal/distal axis small (duodenum, jejunum, ileum) (2) between intestinal segments their respective lymphoid organs (Peyer's patches [PP] mesenteric lymph nodes...
We have previously shown that physiological hormone differences related to pregnancy or sex affect the age-related distribution of mononuclear cell populations during murine ageing. To determine whether such changes are involved in functions T cells, we examined secretion major immunoregulatory cytokines (IL-2, IL-4, interferon-gamma (IFN-gamma), IL-3, IL-6 and granulocyte-macrophage colony-stimulating factor (GM-CSF)) vitro concanavalin A-activated spleen cells C57B1/6 mice. The study...
Spontaneous animal tumors appear to be highly suitable models study human oncology and cancer therapy. The aim of this was characterize the clinical histological features hereditary melanocytic lesions found in French herd melanoblastoma-bearing Libechov minipigs (MeLiM) their Duroc crossbreeds. Clinically, we discriminated between three types skin lesions, which offer a lesion continuum from lentigo metastatic melanomas. More than 70% these on piglets before they are 3 months old...
Metastatic melanoma is a severe disease. Few experimental animal models of metastatic exist. MeLiM minipigs exhibit spontaneous melanoma. Cutaneous and lesions are histologically similar to human's. However, most them eventually spontaneously regress. Our purpose was investigate whether the model could reveal markers malignancy in human melanocytic proliferations. We compared serial analysis gene expression (SAGE) between normal pig skin melanocytes cells from an early pulmonary metastasis...
Incomplete spontaneous regression of melanoma is common. However, complete still a very rare phenomenon. Because the most immunogenic human malignancy, mechanisms leading to regression, based on accumulative evidence, are host's immune responses. Unfortunately, therapies aiming enhance patient's natural immunity against have yet meet their expectations. Reasons for failure include various escape mechanisms, induced by tumor, that subsequently lead tolerance. Here, we performed time-dependent...
Abstract In human familial melanoma, 3 risk susceptibility genes are already known, CDKN2A , CDK4 and MC1R . However, various observations suggest that other melanoma have not yet been identified. To search for new loci, we used the MeLiM swine as an animal model of hereditary to perform a genome scan linkage melanoma. Founders affected stock were crossed with each healthy Duroc pigs, generating MeLiM, F1 backcross families. As had previously excluded gene, paid special attention well...
We previously reported that the olfactory receptor OR51E2, overexpressed in LNCaP prostate cancer cells, promotes cell invasiveness upon stimulation of its agonist β-ionone, and this phenomenon increases metastatic spread. Furthermore, we showed induced involves a PI3 kinase dependent signalling pathway. report here results new investigation to address whether gallein, small inhibitor G protein βγ subunit interaction with kinase, can inhibit β-ionone effects both vitro vivo. demonstrate...
SUMMARY So far all studies on the murine ageing process have been conducted virgin mice. Immune may be influenced by sex hormone differences related to or pregnancies. The aim of this study was investigate whether pregnancies and gender influence cell changes observed during in a peripheral lymphoid compartment C57Bl/6 Using flow cytometry, (Thy1.2+) T cell, (B220+) B (CD11b/Mac-1) macrophage spleen populations were monitored 2, 8 (3 months after last pregnancy) 15 23-month-old mice...
Despite major advances, it is estimated that a large part of melanoma predisposing genes remains to be discovered. Animal models spontaneous diseases are valuable tools and experimental crosses can used identify fine-map new susceptibility loci associated with melanoma. We performed Genome-Wide Association Study (GWAS) occurrence progression (clinical ulceration presence metastasis) in porcine model melanoma, the MeLiM pig. Five on chromosomes 2, 5, 7, 8 16 showed genome-wide significant...
Immunological research in pigs benefits from many improvements with a direct impact on the veterinary control of pig husbandry and biomedical models. We compiled available knowledge to develop gating strategies monitor simultaneously all blood immune cell types by multicolor flow cytometry Melanoblastoma-bearing Libechov Minipigs (MeLiM). The MeLiM spontaneously develops cutaneous melanomas that regress few months later. monitored lymphoid myeloid subsets 3 21 week-old pigs. Interestingly,...
Abstract The human cutaneous metastatic melanoma is the deadliest skin cancer. Partial, or less frequently complete spontaneous regressions could be observed, mainly mediated by T cells. Nevertheless, underlying mechanisms are not fully unraveled. We investigated first events of immune response related to cancer regression in Melanoma-bearing Libechov Minipigs (MeLiM), a unique swine model that regresses spontaneously. Using multiparameter flow cytometry strategy and integrating new clinical...
Abstract Some herds of miniature swine are genetically predisposed to cutaneous melanoma. To test if melanoma susceptibility could be linked the CDKN2A gene, which is involved in a proportion 9p21‐linked human familial melanoma, we performed genetic analysis pigs MeLiM strain. F 1 and backcross animals were generated by crossing boar with healthy Duroc sows. We isolated gene characterized informative microsatellite marker, S0644 marker. Using this marker 2 flanking markers, analyzed...
Abstract Genetic alterations implicated in malignant melanoma are still poorly understood. Malignant melanomas present highly variable histologic and cytologic patterns. The aim of the study is to define genomic imbalances associated with development 2 types swine hereditary cutaneous melanoma. We have investigated 11 tumors by comparative hybridization (CGH), 4 superficial spreading (SSMs) 7 nodular (NMs). Following laser capture microdissection degenerate oligonucleotide primed‐polymerase...
In pig, very little information is available on the non classical class I (Ib) genes of Major Histocompatibility Complex (MHC) i.e. SLA-6, -7 and -8. Our aim was to focus transcription pattern SLA-7 gene. RT-PCR experiments were carried out with specific primers targeting either full coding sequence (CDS) from exon 1 3 prime untranslated region (3UTR) or a partial CDS 4 3UTR. We show that gene expresses length transcript not yet identified refines annotation eight exons instead seven as...
Abstract In MeLiM minipigs, melanomas develop around birth, can metastasize, and have histopathologic characteristics similar to humans. Interestingly, eventually regress. This favorable outcome raises the question of their malignancy, which we investigated. We clinically followed tens tumors from onset first signs regression. Transcriptome analysis revealed an enrichment all cancer hallmarks in melanomas, although no activating or suppressing somatic mutation were found common driver genes....