A5100458267- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Single-cell and spatial transcriptomics
- Immune cells in cancer
- Cytomegalovirus and herpesvirus research
- Microfluidic and Bio-sensing Technologies
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- Cell Image Analysis Techniques
- RNA Interference and Gene Delivery
- vaccines and immunoinformatics approaches
- interferon and immune responses
- Reproductive System and Pregnancy
- Acute Myeloid Leukemia Research
- CAR-T cell therapy research
- Microfluidic and Capillary Electrophoresis Applications
- Hepatitis C virus research
- Complement system in diseases
- Psoriasis: Treatment and Pathogenesis
- Advanced biosensing and bioanalysis techniques
- Parasite Biology and Host Interactions
- Reproductive tract infections research
State Key Laboratory of Oncogene and Related Genes
2019-2024
Shanghai Jiao Tong University
2018-2024
National Cancer Center of Georgia
2024
Walker (United States)
2024
Shanghai First People's Hospital
2023-2024
Shanxi Medical University
2024
Second Hospital of Shanxi Medical University
2024
XinHua Hospital
2024
Augusta University Health
2023
Sinovac Biotech
2023
Abstract Background With the expanding applications of mass cytometry in medical research, a wide variety clustering methods, both semi-supervised and unsupervised, have been developed for data analysis. Selecting optimal method can accelerate identification meaningful cell populations. Result To address this issue, we compared three classes performance measures, “precision” as external evaluation, “coherence” internal stability, nine methods based on six independent benchmark datasets....
Effective capture and analysis of a single circulating tumor cell (CTC) is instrumental for early diagnosis personalized therapy tumors. However, due to their extremely low abundance susceptibility interference from other cells, high-throughput isolation, enrichment, single-cell-level functional protein CTCs within one integrated system remains major challenge. Herein, we present an multifunctional microfluidic highly efficient label-free CTC single-cell immunoblotting (ieSCI). The...
The absence of Caspase-8 or its adapter, Fas-associated death domain (FADD), results in activation receptor interacting protein kinase-3 (RIPK3)- and mixed-lineage kinase-like (MLKL)-dependent necroptosis vivo. Here, we show that spontaneous RIPK3, phosphorylation MLKL, Caspase-8- FADD-deficient cells was dependent on the nucleic acid sensor, Z-DNA binding protein-1 (ZBP1). We genetically engineered a mouse model by single insertion FLAG tag onto N terminus endogenous MLKL (
Alcohol consumption accelerates liver damage and diminishes the anti–hepatitis C virus (HCV) effect of interferon alfa (IFN–α) in patients with HCV infection. It is unknown, however, whether alcohol enhances replication promotes disease progression. The availability replicon containing hepatic cells has provided a unique opportunity to investigate interaction between expression. We determined RNA expression cells. Alcohol, concentration–dependent fashion, significantly increased also...
Abstract Background Leukemias driven by activated, chimeric FGFR1 kinases typically progress to AML which have poor prognosis. Mouse models of this syndrome allow detailed analysis cellular and molecular changes occurring during leukemogenesis. We used these determine the effects leukemia development on immune cell composition in microenvironment progression. Methods Single RNA sequencing (scRNA-Seq) was characterize associated neutrophils define gene expression cells Results scRNA-Seq...
Abstract Natural killer (NK) cells are critical in host innate defense against certain viruses. The role of NK controlling hepatitis C virus (HCV) remains obscure. We examined whether capable inhibiting HCV expression human hepatic cells. When cultured with the replicon-containing cells, they have no direct cytolytic effect but release soluble factor(s) suppressing RNA expression. Media conditioned by cell lines (NK-92 and YTS) or primary isolated from healthy donors contain interferon γ...
Excessive neutrophil migration across the pulmonary endothelium into lung and release of oxidants proteases are key elements in pathogenesis acute injury. Previously, we identified protein kinase C-delta (PKCδ) as an important regulator proinflammatory signaling human neutrophils demonstrated that intratracheal instillation a TAT-conjugated PKCδ inhibitory peptide (PKCδ-TAT) is protective rat model sepsis-induced indirect injury (cecal ligation puncture). In present study, this inhibitor...
Circulating tumor cells (CTCs) are rare existing in the bloodstream with a relatively low number, which facilitate as predictor of cancer progress. However, it is difficult to obtain highly purified intact CTCs desired viability due percentage among blood cells. In this work, we demonstrate novel self-amplified inertial focused (SAIF) microfluidic chip that enables size-based, high-throughput, label-free separation from patient's blood. The SAIF introduced study demonstrated feasibility an...
Abstract Single cell western blot (scWB) is one of the most important methods for cellular heterogeneity profiling. However, current scWB based on conventional photoactive polyacrylamide hydrogel material suffers from tradeoff between in‐gel probing and separation resolution. Here, a highly sensitive temperature‐controlled single‐cell blotting (tc‐scWB) method introduced, which thermo/photo‐dualistic‐sensitive hydrogel, namely acrylic acid‐functionalized graphene oxide (AFGO) assisted,...
Ovarian cancer is highly malignant with a gradually increasing incidence and high mortality rate. Immunosuppression induced in ovarian cancer, although the mechanism detail not clear. It has been indicated that HVEM (herpesvirus entry mediator) B- T-lymphocyte attenuator (BTLA) negatively regulates immune responses of T lymphocytes. Here, mRNA was found to be elevated tissue samples primary cells comparison benign samples. We then knocked down expression an cell line, OVCAR3, by...
T cell immunity is traditionally assessed through functional recall assays, which detect the consequences of cells' antigen encounter, or via fluorescently labeled multimers that selectively bind peptide-specific receptors. Using either approach, if wrong peptide a complex antigenic system, such as virus, used for immune monitoring, false negative data will be obtained, magnitude antigen-specific compartment go largely underestimated. In this work, we show how selection "right" peptides...
Schistosomiasis remains one of the major neglected tropical diseases (NTDs) causing morbidity humans residing in countries. Much effort has been devoted to development vaccines, since it is recognized that vaccines can be served as an important supplementary component alongside chemotherapy for future control and elimination schistosomiasis. To accelerate digging new potential target antigens, essential extensively intensively search immunogenic proteins a high-throughput manner using...
Colon cancer (CC) is one of the leading causes related mortality. Research over past decades have profoundly enhanced our understanding immunotherapy, a major clinical accomplishment, and its potential role towards treating CC. However, studies investigating expression these immune checkpoints, such as epithelial cell adhesion molecule (EpCAM), programmed death-1 (PD-1), death-ligand 1 (PD-L1), by peripheral blood mononuclear cells (PBMCs) lacking. Here, high-dimensional mass cytometry...
We used four-color ImmunoSpot® assays, in conjunction with peptide pools that cover the sequence of tyrosinase (Tyr), melanoma-associated antigen A3 (MAGE-A3), melanocyte antigen/melanoma recognized by T cells 1 (Melan-A/MART-1), glycoprotein 100 (gp100), and New York esophageal squamous cell carcinoma-1 (NY-ESO-1) to characterize melanoma (MA)-specific CD8 + repertoire PBMC 40 healthy human donors (HD). Tyr triggered interferon gamma (IFN-γ)-secreting 25% HD within 24 h stimulation ex vivo....
Imaging mass cytometry (IMC) is an emerging imaging technology that exploits the multiplexed analysis capabilities of CyTOF cytometer to make spatially resolved measurements for tissue sections. In a comprehensive view composition and marker distribution, recent developments IMC require highly sensitive, assays. Approaching sensitivity technique, we designed novel type biocompatible metal-labeled aptamer nanoprobe (MAP), named 167Er-A10-3.2. The small molecular probe was synthesized by...
Abstract Tacrolimus (FK506) and rapamycin (RAPA) are widely used to maintain long‐term immunosuppression after organ transplantation. However, the impact of accumulative drug administration on recipients’ immune systems remains unclear. We investigated 3‐year FK506 or RAPA treatment renal transplantation human systems. A discovery cohort 30 patients was first recruited, we discovered two distinctive T lineage suppressive regulatory patterns induced by chronic RAPA. The increased percentage...
Abstract Co‐detection of multiplex cancer subtypes and bacteria in situ is crucial for understanding tumor microbiome interactions microenvironment. Current standard techniques such as immunohistochemical staining immunofluorescence are limited their multiplicity. Simultaneously visualizing detailed cell distribution across the same pathological section remains a major technical challenge. Herein, we developed rapid semi‐quantitative method imaging phenotypes on solid tissue sections. We...
Plasmacytoid dendritic cells (pDCs) secrete large amounts of IFN-alpha upon exposure to virus, subsequently promoting and regulating innate adaptive immune responses. However, little is known about the functional regulation virus-activated pDCs after they exert functions in secondary lymph organs. Our previous studies show that splenic stromal microenvironment can down-regulate T cell response by inducing generation regulatory myeloid cells; therefore, we wondered whether regulate function...