A5024601621- Epigenetics and DNA Methylation
- Effects and risks of endocrine disrupting chemicals
- Histone Deacetylase Inhibitors Research
- Reproductive Biology and Fertility
- Retinoids in leukemia and cellular processes
- Pesticide Exposure and Toxicity
- Pharmacological Effects and Toxicity Studies
- Pesticide and Herbicide Environmental Studies
- Birth, Development, and Health
- Craniofacial Disorders and Treatments
- Folate and B Vitamins Research
- Pregnancy and Medication Impact
- Prenatal Substance Exposure Effects
- Neurogenetic and Muscular Disorders Research
- Carcinogens and Genotoxicity Assessment
- Pluripotent Stem Cells Research
- Reproductive System and Pregnancy
- Cleft Lip and Palate Research
- Gestational Diabetes Research and Management
- Genetics and Neurodevelopmental Disorders
- Gestational Trophoblastic Disease Studies
- Animal Genetics and Reproduction
- Developmental Biology and Gene Regulation
- Pregnancy and preeclampsia studies
- Infant Nutrition and Health
University of Milan
2016-2025
Institute of Biosciences and Bioresources
2015
BACKGROUND: the inhibition of histone deacetylase (HDAC) has been reported as an effective mechanism on therapy in neoplastic diseases. Among HDAC inhibitors, Trichostatin A (TSA) and Valproic Acid (VPA) prevent tumorigenesis rodent human models. Malformations neural tube axial skeletal defects are well-known VPA side effects. Recent hypotheses suggest inhibitor activity teratogenic VPA. The potency TSA is, at moment, unknown. aim present work is to investigate embryos exposed utero or...
Fetal Alcohol Spectrum Disorders (FASD) refer to a range of conditions in children caused by alcohol consumption during pregnancy, including morphological defects, developmental delays, and neurobehavioral impairments. Ethanol (EtOH) at high concentrations (1-3% v/v) was shown induce malformations lethality Xenopus laevis embryos exposed throughout the FETAX test (from mid-blastula stage final pre-feeding larval stage). The aim this work evaluate multiple effects EtOH exposure (0.1-3% using...
Abstract Background The double staining of fetal skeleton for bone and cartilage is a very useful method to evidence skeletal abnormalities in laboratory animals. However, this has been rarely used routine developmental toxicity tests. One reason could be the difficulty comparing single pieces having reference points. In paper rat stained with Alizarin red S Alcyan Blue described detail produce an atlas laboratories. Teratology 64:125–133, 2001. © 2001 Wiley‐Liss, Inc.
The widely accepted dogma of intrauterine sterility and initial colonization the newborn during birth has been blurred by recent observations microbial presence in meconium, placenta, amniotic fluid. Given importance a maternal-derived utero infant seeding, it is crucial to exclude potential environmental or procedural contaminations assess fetal before parturition. To this end, we analyzed sterilely collected intestinal tissues, fluid from rodent fetuses tissues autoptic human fetuses....
The RHAPS (Redox-Activity And Health-Effects Of Atmospheric Primary Secondary Aerosol) project was launched in 2019 with the major objective of identifying specific properties fine atmospheric aerosol from combustion sources that are responsible for toxicological effects and can be used as new metrics health-related outdoor pollution studies. In this paper, we present overall methodology introduce phenomenology first data observed. A comprehensive physico-chemical characterization has been...
EFSA established cumulative assessment groups and conducted retrospective risk assessments for two types of craniofacial alterations (alterations due to abnormal skeletal development, head soft tissue brain neural tube defects) 14 European populations women in childbearing age. Cumulative acute exposure calculations were performed by probabilistic modelling using monitoring data collected Member States 2017, 2018 2019. A rigorous uncertainty analysis was expert knowledge elicitation....
Bisphenol A (BPA) is a plastic additive with endocrine disruptive activity, classified in 2017 by EU ECHA as substance of very high concern. correlation between environmental exposure to BPA and congenital defects has been described humans experimental species, including the amphibian Xenopus laevis. Among analogues, bisphenol B (BPB) used alternative different not-EU countries, US, but seems share its disruptor properties. Aim present work evaluation effects BPB versus X. laevis...
This experiment was carried out with the aims of comparing embryotoxic potential valproic acid (VPA) in rats and mice, better defining malformation pattern these species, effects detectable mid-pregnancy to those observed fetuses at term. Pregnant DC:Crl were treated subcutaneously (s.c.) 08:00, 16:00, 00:00 on day 9 gestation 0, 150, 300 mg/kg VPA; pregnant NMRI mice s.c. 7 gestation, 08:00 16:00 8 75, or VPA. Groups females killed (mice) 11 (rats) pregnancy their embryos carefully examined...
Abstract BACKGROUND: Triadimefon is an antifungal derived from triazole. In in vitro whole‐rodent embryo cultures, triazole‐derivatives showed specific teratogenic effects at the branchial apparatus. The aim of present work was to test vivo triadimefon (FON), order verify a relationship between triazole exposure, embryonic abnormalities, and/or fetal malformations. METHODS: Pregnant CD‐1 mice were treated with 0–300 mg/kg FON by gavage on day 8 post coitum (p.c.) 10:00 AM , and sacrificed...
Objectives Valproic acid (VPA) is a widely used anticonvulsant medication with well-known teratogenic effects in both humans and experimental animal model systems. The most commonly observed malformations induced by VPA animals include neural skeletal defects. In this study the potential alterations somitic tissue gene expression relative to development of axial defects were examined. Methods SWV mice treated at 8.5 days post coitum (d.p.c.) 1.36 mmol/kg or 2.72 i.p. injection. At 18.5...