A5033314672- Clinical Laboratory Practices and Quality Control
- Advanced biosensing and bioanalysis techniques
- Liver Disease Diagnosis and Treatment
- Reliability and Agreement in Measurement
- Statistical Methods in Clinical Trials
- DNA and Nucleic Acid Chemistry
- Renal function and acid-base balance
- Whipple's Disease and Interleukins
- Adipokines, Inflammation, and Metabolic Diseases
- IL-33, ST2, and ILC Pathways
- Meta-analysis and systematic reviews
- Psoriasis: Treatment and Pathogenesis
- Blood groups and transfusion
- Hemoglobinopathies and Related Disorders
- Hemodynamic Monitoring and Therapy
- CRISPR and Genetic Engineering
- Blood properties and coagulation
- Hepatitis B Virus Studies
- Advanced Statistical Process Monitoring
- Single-cell and spatial transcriptomics
- Renal and Vascular Pathologies
- Statistical Methods in Epidemiology
- Iron Metabolism and Disorders
- Liver Diseases and Immunity
- Bacteriophages and microbial interactions
First Hospital of China Medical University
2014-2025
China Medical University
2014-2025
Background Complete blood count (CBC) reference intervals are important to diagnose diseases, screen donors, and assess overall health. However, current established by older instruments technologies those from American European populations not suitable for Chinese samples due ethnic, dietary, lifestyle differences. The aim of this multicenter collaborative study was establish CBC healthy Han adults. Methods A total 4,642 individuals (2,136 males 2,506 females) were recruited six clinical...
Background Reference intervals of Liver function tests are very important for the screening, diagnosis, treatment, and monitoring liver diseases. We aim to establish common reference specifically Chinese adult population. Methods A total 3210 individuals (20–79 years) were enrolled in six representative geographical regions China. Analytes ALT, AST, GGT, ALP, protein, albumin bilirubin measured using three analytical systems mainly used The newly established based on results traceability or...
The autoverification system for coagulation consists of a series rules that allow normal data to be released without manual verification. With new advances in medical informatics, the laboratory information (LIS) has growing potential autoverification, allowing rapid and accurate verification clinical tests. purpose study is develop evaluate LIS-based validation efficiency. Autoverification decision rules, including quality control, analytical error flag, critical value, limited range check,...
Background Reference intervals (RIs) play an important role in clinical decision-making. However, due to the time, labor, and financial costs involved establishing RIs using direct means, use of indirect methods, based on big data previously obtained from laboratories, is getting increasing attention. Different techniques combined with different transformation methods outlier removal might cause differences calculation RIs. there are few systematic evaluations this. Objective This study used...
Background Currently there are no reference intervals (RIs) of sodium (Na), potassium (K), and chlorine (Cl) on Chinese population. Two kinds ion‐selective electrode (ISE) methods were commonly used to determine K, Na, Cl levels in China, the difference between these two needs be evaluated. Methods A total 4,524 healthy participants (1,916 males 2,608 females) 20–79 years old from six cities China selected by strict criteria. Serum CL tested Roche Modular analyzers assigned laboratories....
A large number of people undergo annual health checkup but accurate laboratory criterion for evaluating their status is limited. The present study determined biological variation (BV) and derived parameters common analytes in order to accurately evaluate the test results healthcare population.A total 43 healthy individuals who had regular once a year six consecutive years, were enrolled using physical, electrocardiogram, ultrasonography laboratory. BV data parameters, such as reference...
The Pediatric Reference Intervals in China (PRINCE) was initiated to establish the reference intervals (RIs) of Chinese children, as well make it possible compare variability biochemical markers among countries internationally.
Abstract Background There is no common consensus on how to define the reference population for determination of high‐sensitivity cardiac troponin (hs‐cTn) upper limit (URL). This study aimed establish 99th percentile URLs hs‐cTnT under both 2018 AACC/IFCC criteria and improved selection further judging whether two are different. Methods Applying stratified cluster sampling protocol, this took 1848 apparently healthy subjects in communities Shenyang China as screening objects. We first...
To further understand the ligation mechanism, effects of 2'-O-methyl nucleotide (2'-OMeN) on T4 DNA efficiency were investigated. Fluorescence resonance energy transfer assay was used to monitor nick-joining process by ligase. Results showed that substitutions at 5'- and 3'-ends nick decreased 48.7% ± 6.7% 70.6% 4.0%, respectively. Substitutions both 76.6% 1.3%. Corresponding kinetic parameters, Vmax, Km, kcat, have been determined in each case using Michaelis-Menten equation. The data...
To investigate the effect of sugar pucker conformation on DNA-protein interactions, we used 2'-O-methyl nucleotide (2'-OMeN) to modify EcoRI recognition sequence -TGAATTCT-, and monitored enzymatic cleavage process using FRET method. The has a C3'-endo different from C2'-endo native DNA nucleotides. initial reaction velocities were measured kinetic parameters, Km Vmax derived Michaelis-Menten equation. Experimental results showed that 2'-OMeN substitutions for decreased efficiency A2, A3 T4...
Abstract Mitosis promoting factor (MPF) plays a central role during the first mitosis of mouse embryo. We demonstrated that MPF activity increased when one‐cell stage embryo initiated G2/M transition following decrease cyclic adenosine 3′, 5′‐monophosphate (cAMP) and cAMP‐dependent protein kinase (PKA) activity. When cAMP PKA increases again, decreases starts metaphase–anaphase transition. In downstream cAMP/PKA, there are some effectors such as polo‐like 1 (Plk1), Cdc25, Mos...
Inflammatory/immune cells have the power of infiltrating almost all human solid tumors and influencing stages carcinogenesis because their stimulation various cytokine subsets. This study aims to determine correlation single nucleotide polymorphisms in IL-17F gene risk colorectal cancer (CRC). One thousand patients diagnosed with CRC a control group 354 healthy controls were involved. Peripheral blood samples collected. The PCR-RFLP method was used detect 7383A>G (rs2397084) 7488T>C...
The effects of nucleotide analogue substitution on the cleavage efficiencies type II restriction endonucleases have been investigated. Six (EcoRV, SpeI, XbaI, XhoI, PstI and SphI) were investigated respectively regarding their when substrates substituted by 2′-O-methyl (2′-OMeN) phosphorothioate (PS). Substitutions made in recognition sequence two nucleotides flanking for each endonuclease. endonuclease determined using FRET-based assay. Results demonstrated a position-dependent inhibitory...
Background Serum total N-terminal propeptide of type I collagen (P1NP) and serum C-terminal telopeptide (CTX) are used as reference analytes bone turnover in clinical application. Biological variation is important for application these biomarkers. However, the biological data biomarkers not consistent. The present study determined variations P1NP CTX their confidence intervals China using electrochemiluminescence. Methods We collected samples from 25 healthy individuals (17 women 8 men,...
Background The aim of our study was to assess two tumor markers, carbohydrate antigen 724 ( CA 724) and neuron‐specific enolase NSE ), in 20 healthy individuals. Then, we determined their average intra‐ inter‐individual biological variations CV I G ). Methods Blood samples were taken at weekly intervals for five weeks from subjects through a detailed questionnaire physical examination. These stored −80°C analyzed single run duplicate. We calculated the variations, reference change value RCV...
Abstract Background Reference intervals (RIs) transference can expand the applicability of established RIs. However, study on methodology is insufficient, and RIs validation based small samples cannot adequately identify transferred risk under complex situations. This aimed to find appropriate conditions ensure effect transference. Methods We Roche Beckman systems for 27 analytes 681 healthy individuals. were converted into using linear regression (least squares method) which divided two...
The data from apparently healthy individuals with thalassemia has been demonstrated to have an effect on the reference intervals for erythrocyte indices in areas a high incidence of thalassemia.Six clinical centers screened using questionnaire and physical examination. Then, qualified were selected by hematological genotypic diagnosis. Statistical comparisons conducted Chinese population without thalassemia. constituent ratios mean (SD) according genotype calculated. relationship between MCV...
Abstract Objectives Peripheral blood lymphocyte subsets are important parameters for monitoring immune status; however, subset detection is time-consuming and error-prone. This study aimed to explore a highly efficient clinically useful autoverification system assays performed on the flow cytometry platform. Methods A total of 94,402 test results were collected. To establish limited-range rules, 80,427 first used (69,135 T tests 11,292 NK, B, tests), which 15,000 from human immunodeficiency...