A5029646733- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- Spaceflight effects on biology
- Mitochondrial Function and Pathology
- Olfactory and Sensory Function Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Tryptophan and brain disorders
- Retinal Development and Disorders
- Environmental Monitoring and Data Management
- Neurobiology and Insect Physiology Research
- Biochemical Analysis and Sensing Techniques
- Neuroendocrine regulation and behavior
- Ethics in medical practice
- Pluripotent Stem Cells Research
- Planarian Biology and Electrostimulation
- Health and Medical Research Impacts
- Adipose Tissue and Metabolism
- Ethics in Business and Education
- Exercise and Physiological Responses
- Cellular Mechanics and Interactions
- Muscle Physiology and Disorders
- Neural dynamics and brain function
- CRISPR and Genetic Engineering
University of Washington
2020-2024
Fred Hutch Cancer Center
2019-2024
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
2021-2022
Cancer Research Center
2021-2022
Seattle University
2021
Brotman Baty Institute
2020-2021
Rockefeller University
2008-2020
University of California, Berkeley
2004-2014
Sensory neurons are an animal's gateway to the world, and their receptive endings, sites of sensory signal transduction, often associated with glia. Although glia known promote sensory-neuron functions, molecular bases these interactions poorly explored. Here, we describe a post-developmental glial role for PROS-1/Prospero/PROX1 homeodomain protein in function C. elegans. Using expression profiling, demonstrate that, unlike previously characterized cell fate roles, PROS-1 functions...
Glia in the central nervous system engulf neuron fragments to remodel synapses and recycle photoreceptor outer segments. Whether glia passively clear shed neuronal debris or actively prune is unknown. How pruning of single-neuron endings impacts animal behavior also unclear. Here, we report our discovery glia-directed Caenorhabditis elegans. Adult C. elegans AMsh sensory AFD thermosensory by repurposing components conserved apoptotic corpse phagocytosis machinery. The phosphatidylserine (PS)...
For an organ to maintain correct architecture and function, its diverse cellular components must coordinate their size shape. Although cell-intrinsic mechanisms driving homotypic cell-cell coordination are known, it is unclear how cell shape regulated across heterotypic cells. We find that epithelial cells the of neighboring sense-organ glia-neuron units in adult Caenorhabditis elegans (C. elegans). Hsp co-chaperone UNC-23/BAG2 prevents from deforming, loss causes head epithelia stretch...
Glia interact with multiple neurons, but it is unclear whether their interactions each neuron are different. Our interrogation at single-cell resolution reveals that a single glial cell exhibits specificity in its different contacting neurons. Briefly, C. elegans amphid sheath (AMsh) glia apical-like domains contact 12 neuron-endings. At these ad-neuronal membranes, AMsh localize the K/Cl transporter KCC-3 to microdomain exclusively around thermosensory AFD regulate properties. Glial...
A comprehensive description of nervous system function, and sex dimorphism within, is incomplete without clear assessment the diversity its component cell types, neurons glia. C. elegans has an invariant with first mapped connectome a multi-cellular organism single-cell atlas neurons. Here we present single nuclear RNA-seq evaluation glia across entire adult system, including both sexes. Machine learning models enabled us to identify sex-shared sex-specific glial subclasses. We have...
Significance Duchenne muscular dystrophy is a degenerative disease affecting tens of thousands people in the United States alone. Much remains unknown about disease, including chain events that links loss dystrophin to muscle death, and extent which exercise might be able protect degenerating muscles. We used nematode Caenorhabditis elegans show sarcoplasmic calcium dysregulation takes place dystrophic muscles before other overt signs damage manifest. When placed assays altered activity by...
Age-dependent cognitive and behavioral deterioration may arise from defects in different components of the nervous system, including those neurons, synapses, glial cells, or a combination them. We find that AFD, primary thermosensory neuron Caenorhabditis elegans, aged animals is characterized by loss sensory ending integrity, reduced actin-based microvilli abundance aggregation guanylyl cyclases. At functional level, AFD neurons are hypersensitive to high temperatures show sustained...
Abstract Cytohesins are Arf guanine nucleotide exchange factors (GEFs) that regulate membrane trafficking and actin cytoskeletal dynamics. We report here GRP-1, the sole Caenorhabditis elegans cytohesin, controls asymmetric divisions of certain neuroblasts divide to produce a larger neuronal precursor or neuron smaller cell fated die. In Q neuroblast lineage, loss GRP-1 led production daughter cells more similar in size transformation normally apoptotic into its sister, resulting extra...
The outcome of deliberation on various aspects publication and free access to scientific literature by a panel nominated fellows from three science academies, viz. Indian National Science Academy, Academy Sciences, Sciences India, expert invitees are presented.
Understanding how neurons adopt particular fates is a fundamental challenge in developmental neurobiology. To address this issue, we have been studying Caenorhabditis elegans lineage that produces the HSN motor neuron and PHB sensory neuron, sister cells produced by HSN/PHB precursor. We previously shown novel protein HAM-1 controls asymmetric neuroblast division lineage. In study examine tbx-2 egl-5, genes act concert with ham-1 to regulate fate. screens for mutants abnormal development,...
ABSTRACT For an organ to maintain proper architecture and function, its different component cell-types must coordinate their cell-shapes with each other through life. While cell-intrinsic developmental mechanisms driving homotypic cell-cell coordination are known, how heterotypic cells collectively regulate cell-shape is less-clear. We report that, in a sense-organ, epithelial delimit polarity domains of contacting glia, thereby, associated neuron shapes throughout Briefly, Hsp co-chaperone...
In the following pages, we share a collection of photos, drawings, and mixed-media creations, most them especially made for this JoN issue, manifesting C. elegans researchers' affection their model organism founders field. This is celebration our community's growth, flourish, spread, bright future. Descriptions provided by contributors, edited space. 1.
Thermotaxis behaviors in C. elegans exhibit experience-dependent plasticity of thermal preference memory. This behavior can be assayed either at population level, on linear temperature gradients, or the individual animal by radial isothermal microfluidic tracking orientation. These are low-throughput as well variable, due to inherent sensitivity environmental perturbations. To facilitate reproducible studies, we describe an updated apparatus design that enables simultaneous runs three...