A5047408416- Spaceflight effects on biology
- Calcium signaling and nucleotide metabolism
- High Altitude and Hypoxia
- Sphingolipid Metabolism and Signaling
- Lipid Membrane Structure and Behavior
- Sleep and Wakefulness Research
- Autophagy in Disease and Therapy
- Traumatic Brain Injury and Neurovascular Disturbances
- Cancer, Hypoxia, and Metabolism
- Heme Oxygenase-1 and Carbon Monoxide
- Ion Channels and Receptors
- Ion channel regulation and function
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Circadian rhythm and melatonin
- Anesthesia and Neurotoxicity Research
- Nitric Oxide and Endothelin Effects
- Lipid metabolism and disorders
- Birth, Development, and Health
- Noise Effects and Management
- Human Health and Disease
- Genetics, Aging, and Longevity in Model Organisms
- Epilepsy research and treatment
- Adenosine and Purinergic Signaling
- Advanced Glycation End Products research
Air Force Medical University
2013-2024
Virginia Commonwealth University
2016-2017
Virginia Commonwealth University Medical Center
2009-2015
Abstract Although haemoglobin is a known carrier of oxygen in erythrocytes that functions to transport over long range, its physiological roles outside are largely elusive 1,2 . Here we found chondrocytes produced massive amounts form eosin-positive bodies their cytoplasm. The body (Hedy) membraneless condensate characterized by phase separation. Production controlled hypoxia and dependent on KLF1 rather than the HIF1/2α pathway. Deletion leads Hedy loss along with severe hypoxia, enhanced...
Previous studies have demonstrated activation of the local renin-angiotensin system in hindlimb unweighting (HU) rat vasculature. The present study intended to identify effects blockade angiotensin II (ANG II) type 1 (AT(1)) receptors with losartan on vascular reactivity, nitric oxide synthase (NOS) expression, and superoxide anion (O(2)(*-)) levels 3-wk HU cerebral carotid arteries. Three weeks later, vasoconstriction, vasodilatation, endothelial NOS (eNOS) inducible (iNOS) protein, as well...
This study explored the mechanism mediating aggregation of membrane NADPH oxidase (NOX) subunits and subsequent activation this enzyme in bovine coronary arterial endothelial cells (CAECs). With confocal microscopy, we found that FasL stimulated lipid rafts (LRs) clustering with NOX subunit acid sphingomyelinase (ASM) gathering, which was blocked by siRNA sortilin, an intracellular protein responsible for binding targeting ASM to lysosomes. Correspondingly, FasL-induced O(2)(.-) production...
This study was designed to test the hypothesis that a 28-day tail suspension (SUS) could induce hypertrophy and enhanced myogenic vasoconstrictor reactivity in middle cerebral arteries (MCAs), whereas atrophy decreased responses mesenteric third-order arterioles (MSAs). Also, addition functional enhancement MCAs, structural changes both kinds of decrement MSAs all be prevented by intervention daily 1-h dorsoventral (-G(x)) gravitation restoring standing posture. To this hypothesis, vessel...
BackgroundNot all the people with metabolic syndrome (MS) have abdominal obesity (AO). The study aimed to investigate gender differences in prevalence and development of MS Chinese population obesity, which has rarely been reported. MethodsData were obtained from 2007-08 China National Diabetes Metabolic Disorders Study, participants divided into two samples for analysis. Sample 1 consisted 19,046 while sample 2 included 2,124 meeting pre-specified requirements. Survival analysis was used...
The present study tested the hypothesis that homocysteine (Hcys)-induced ceramide production stimulates lipid rafts (LRs) clustering on membrane of glomerular endothelial cells (GECs) to form redox signalling platforms by aggregation and activation NADPH oxidase subunits thereby enhances superoxide (O2*-) production, leading dysfunction ultimate injury or sclerosis. Using confocal microscopy, we first demonstrated a co-localization LR clusters with subunits, gp91(phox) p47(phox) in GECs upon...
The present study determined whether activation of acid sphingomyelinase (ASM) drives membrane proximal lysosomes to fuse the cell surface, facilitating lipid rafts (LRs) clustering in coronary arterial endothelial cells (CAECs) and leading dysfunction. By confocal microscopy, activators ASM, phosphatidylinositol (PI), bis (monoacylglyceryl) phosphate (Bis), an inducer butyrate, were found increase LRs bovine CAECs, which was blocked by lysosome fusion inhibitor vacuolin-1. However, arsenic...
The aim of the present study was to investigate whether an expression vascular cell adhesion molecule–1 (VCAM-1) upregulated in 3-week simulated microgravity rat cerebral and carotid arteries impaired endothelium–dependent relaxation concomitant with VCAM-1 expression. Male Sprague-Dawley rats were randomly divided into control (CON) hindlimb unweighting (HU) groups. After 3 weeks, protein vasodilatation basilar artery common determined. Immunohistochemical results revealed positive staining...
Post-spaceflight orthostatic intolerance is one of the most important adverse effects after exposure to space microgravity, and there are still no effective countermeasures. It has been considered that arterial remodeling may play an role in occurrence post-spaceflight intolerance, but cellular mechanisms remain unknown. In this study, we investigated whether inflammatory response exists common carotid artery rats exposed simulated microgravity. For this, Sprague-Dawley were subjected 4...
Summary Noise‐induced structural and functional disorder of the liver has been realized, but underlying mechanism remains to be characterized, which limited introduction precautious measures. Over‐activation acid sphingomyelinase ( ASM )/ceramide (Cer) pathway takes centre stage in hepatocyte injury entailed by various stimulus. We aimed investigate whether it mediated noise elicited on infrastructure, lipid metabolism, apoptosis, oxidative stress. Mice were exposed broad band (20–20k Hz,...
Collagen deposition is a hallmark of atherosclerosis. Although compromised collagen I degradation has been implied in the pathogenesis atherosclerosis, molecular mechanisms are still unclear. Thus, we determined role CD38, an enzyme involved cellular calcium modulation and autophagic flux, regulation coronary arterial myocytes (CAMs). In primary cultured CAMs from CD38-/- mice, protein accumulation but not mRNA abundance was significantly increased compared with cells CD38+/+ mice either...
Background: Excessive daytime sleepiness (EDS) forms a prevalent symptom of obstructive sleep apnea (OSA) and narcolepsy type 1 (NT1), while the latter might always be overlooked. Machine learning (ML) models can enable early detection these conditions, which has never been applied for diagnosis NT1. Objective: The study aimed to develop ML prediction help non-sleep specialist clinicians identify high probability comorbid NT1 in patients with OSA early. Methods: Totally, clinical features...
Lysosomal exocytosis and fusion to cellular membrane is critical in the oxidative stress formation of endothelium under apoptotic stimulus. We investigated role therein it hyperglycaemia-induced endothelial dysfunction. The lysosome-membrane was shown by expression lamp1, lysosomal marker, on transportation symbolic enzymes into cultural medium. also examined ceramide production, lipid rafts (LRs) clustering, colocalization gp91phox, a NADPH oxidase subunit (NOX) LRs clusters, superoxide...
Microgravity-induced vascular remodelling may play an important role in post-spaceflight orthostatic intolerance. In this study, we aimed to investigate the effects of simulated microgravity on monocyte adhesion aortic endothelium hindlimb unweighted rats and elucidate underlying mechanisms associated with event. Sprague-Dawley were subjected 4-week unweighting simulate microgravity. The recruitment monocytes abdominal aorta was investigated by en face immunofluorescence staining binding...
Introduction: Acute carbon monoxide (CO) poisoning causes serious health problems such as neuropsychological sequelae. This study aimed to investigate neuronal apoptosis and the effects of hyperbaric oxygen (HBO2) on different regions rat hippocampus after CO poisoning. Methods: 90 mature male Sprague Dawley rats were randomly divided into three groups: normal control group (NC group), acute monoxide-poisoned (CO group) treatment (HBO2 group). exposure included 0, 1, 3, 7, 14 21 days, one...
Weightlessness induces the functional remodelling of arteries, but changes to angiotensin II (Ang II)-elicited vasoconstriction and underlying mechanism have never been reported. Caveolae are invaginations cell membrane crucial for contraction vascular smooth muscle cells, so we investigated adaptation Ang II-elicited simulated weightlessness role caveolae in it. The 4 week hindlimb unweighted (HU) rat was used simulate effects weightlessness. measured by isometric force recording....
Noise-induced hearing loss (NIHL) relates closely to auditory cortex (AC) injury, so countermeasures aiming at the AC recovery would be of benefit. In this work, effect hyperbaric oxygen treatment on NIHL was elucidated, which imposed mice before (HBOP), during (HBOD) or after (HBOA) noise exposure. Morphology neurons assayed by hematoxylin-eosin Nissl staining. Ceramide (Cer) level measured through immunohistochemistry analysis. Apoptotic were counted using transferase-mediated dUTP nick...
Neuronal damage in primary auditory cortex (A1) underlies complex manifestations of noise exposure, prevention which is critical for health maintenance. Acid sphingomyelinase (ASM) catalyzes generation ceramide (Cer) if over-activated mediates neuronal disorders various diseases. Tricyclic antidepressants (TCAs), by restraining ASM/Cer, benefits multiple anomalies, so we aimed to elucidate the effect TCA on induced hearing loss and derangement, unraveling mechanism involved. The mice were...