A5052510145- Glioma Diagnosis and Treatment
- Histone Deacetylase Inhibitors Research
- Pharmacological Effects and Toxicity Studies
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Radiomics and Machine Learning in Medical Imaging
- Brain Metastases and Treatment
- Cell Image Analysis Techniques
- Cancer-related molecular mechanisms research
- Gene expression and cancer classification
- Ferroptosis and cancer prognosis
- Epilepsy research and treatment
- Cancer-related cognitive impairment studies
- Neurogenesis and neuroplasticity mechanisms
- Neuroblastoma Research and Treatments
- Bioinformatics and Genomic Networks
- Cytokine Signaling Pathways and Interactions
- HER2/EGFR in Cancer Research
- Mathematical Biology Tumor Growth
- Melanoma and MAPK Pathways
- Computational Drug Discovery Methods
- Extracellular vesicles in disease
- Bladder and Urothelial Cancer Treatments
- Cell Adhesion Molecules Research
- Caveolin-1 and cellular processes
University Medical Center Utrecht
2012-2023
Utrecht University
2019
Heidelberg University
2016-2019
University Hospital Heidelberg
2016-2019
University of Liège
2015-2016
Abstract Background. Patients with glioblastoma (GBM) have an overall median survival of 15 months despite multimodal therapy. These catastrophic rates are to be correlated systematic relapses that might arise from remaining stem cells (GSCs) left behind after surgery. In this line, it has recently been demonstrated GSCs able escape the tumor mass and preferentially colonize adult subventricular zone (SVZ). At a distance initial site, these therefore represent high-quality model clinical...
Epileptogenic glioblastomas are thought to convey a favorable prognosis, either due early diagnosis or potential antitumor effects of antiepileptic drugs. We investigated the relationship between survival and epilepsy at presentation, diagnosis, drug therapy in glioblastoma patients. Multivariable Cox regression was applied data 647 consecutive patients diagnosed with de novo 2005 2013 order investigate association In addition, we quantified valproic acid (VPA) treatment. Epilepsy correlated...
Abstract Background Cognitive impairment is a common and debilitating symptom in patients with diffuse glioma, the result of multiple factors. We hypothesized that molecular tumor characteristics influence neurocognitive functioning (NCF), aimed to identify tumor-related markers NCF glioma patients. Methods examined relation between cognitive performance (executive function, memory, psychomotor speed) intratumoral expression levels treatment-naive glioma. performed single-center study...
Introduction The subventricular zone (SVZ) in the brain is associated with gliomagenesis and resistance to treatment glioblastoma. In this study, we investigate prognostic role biological characteristics of involvement Methods We analyzed T1-weighted, gadolinium-enhanced MR images a retrospective cohort 647 primary glioblastoma patients diagnosed between 2005–2013, performed multivariable Cox regression analysis adjust effect SVZ for clinical patient- tumor-related factors. Protein...
Epilepsy at presentation is an independent favorable prognostic factor in glioblastoma (GBM). In this study, we analyze the oncologic signaling pathways that associate with epilepsy human GBMs, and can underlie effect. Following ethical approval patient consent, fresh frozen GBM tissue was obtained from 76 surgeries. Hospital records were screened for presence of seizures disease. mRNA miRNA expression-based gene set enrichment analyses performed on these tissues, to uncover candidate...
The antiepileptic drug valproic acid (VPA) inhibits histone deacetylase in glioblastoma cells vitro, which influences several oncogenic pathways and decreases glioma cell proliferation. clinical relevance of these observations remains unclear, as VPA does not seem to affect patient survival. In this study, we analyzed whether the vitro effects treatment on acetylation are also observed tumor tissues patients.The were assessed with immunofluorescence western blotting. On tissue microarrays...
Background: Involvement of the subventricular zone (SVZ) in glioblastoma is associated with poor prognosis and specific tumor-biological characteristics. The SVZ microenvironment can influence gene expression cells preclinical models. We aimed to investigate whether has any on intratumoral patterns patients. Methods: publicly available Ivy Glioblastoma database contains clinical, radiological whole exome sequencing data from multiple regions resected glioblastomas. involvement various tissue...
The copy number and mRNA expression of STAT5b were assessed in samples from the TCGA repository glioblastomas (GBM). activation this transcription factor was analyzed on tissue microarrays comprising 392 WHO 2016 GBM our clinical practice. These data correlated with patient survival using multivariable Cox analysis and, for a subset 167 tumors, signs tumor invasiveness MRI. effects knockdown by siRNA growth, therapeutic resistance, invasion migration cell lines U87, U87‑EGFRVIII LN18 primary...
: Epilepsy is common in glioma patients, and glioma-related epilepsy has a strong impact on patients’ quality of life. Glioma-related unfavorable clinical course when compared to other types symptomatic epilepsy, with low rates seizure freedom, relapses seizures after seizure-free periods, severe outcome case intractable (status epilepticus). Translational research starting elucidate the specific pathophysiological mechanisms this disease: molecular-biological characteristics tumor result...
Background: Fractal structure is found throughout many processes in nature, and often arises from sets of simple rules. We examined MRI contrast enhancement patterns glioblastoma patients for evidence fractal correlated these with gene expression patterns.Methods: For 39 patients, volumetric T1-post-contrast scans were obtained before surgical resection. calculated the dimension each tumor’s using a box-counting (cubic scaling) approach. RNA microarray data resected tissue explored by set...
Aim: This study aims to analyze the role of I-kappa-B kinase (IKK)-epsilon in glioblastoma (GBM). Methods: A series vitro, vivo, microarray, and immunohistochemical assessments were performed evaluate biological effects IKK-epsilon on cell signaling, radiation sensitivity, patient survival GBM condition. Results: was strongly expressed 75% 195 primary samples but did not correlate with survival. No correlation established between copy number, messenger RNA (mRNA) expression, protein...
BACKGROUND: The objective was to analyze the role of IkappaB-zeta in cell death resistance and growth glioblastomas. METHODS: CNV, mRNA protein expression NFKBIZ/IKB-zeta were assessed low grade high glioma samples from TCGA, Rembrandt proprietary cohorts tumors, totaling 1195 samples. Data correlated histological patient survival. IKBzeta knocked down using inducible sh-RNA technology GBM cultures, clonogenic assays, MTT counts, caspase activation LDH release Western blotting, FACS DNA...
BACKGROUND: In 30-40% of glioblastoma patients, the disease present with an epileptic seizure. Some studies suggest that tumor-associated epilepsy influences survival possibly due to early diagnosis or tumors' biology. However, in vivo mechanisms underlying suggested prognostic effect patients are still unclear. METHODS: a retrospective cohort study, we investigated on 230 using multivariate Weibull analysis. Gene set enrichment analyses defined transcriptional effects and anti-epileptic...
BACKGROUND: Several studies suggest that involvement of the subventricular zone (SVZ) by glioblastoma is a negative prognostic factor associating to poor patient survival. We confirm this relationship and explore biological significance effect in series 647 consecutive patients. METHODS: performed multivariable Cox regression analysis using retrospective cohort primary GBM SVZ was determined gadolinium-enhanced T1 pre-operative MRI scans. The adjusted for age, gender, KPS, surgery type,...
INTRODUCTION: Fractal structure is found throughout many processes in nature, and often arises from sets of simple rules. We examined the contrast enhancement pattern glioblastoma brain tumor MRIs for evidence fractal structure, which might then be compared with expression specific gene obtained surgical specimens each tumor. METHODS: Volumetric T1 postcontrast imaging was 39 patients prior to resection pathology-confirmed lesions. For tumor, we calculated dimension (Minkowski Bouligand...
2057 Background: Epilepsy at presentation is a favorable prognostic factor in glioblastoma. In literature, deregulated expression of serum response (SRF) has been associated with epilepsy animal models, and seems to enhance proliferation invasion multiple types cancer. this study, we analyze the biological mechanisms that associate glioblastoma patients, which might thus underlie effect. Methods: Molecular classification, mRNA miRNA expression-based (gene set) enrichment analyses were...
Despite high expression of the angiogenic stimulator VEGF-A and recently reported autocrine VEGF-A-
AbstractIntroduction:In glioblastoma, VEGF expression promotes angiogenesis and has been reported to correlate with contrast enhancement on MRI scans. However, clinical results of anti-angiogenic therapy seem limited there is a need identify glioblastoma patients that might benefit from this treatment. Fractal structure found throughout many processes in nature, often arises sets simple growth rules. In study, we examined the patterns scans for evidence fractal correlated these biological...
Fractal structure is found throughout many processes in nature, and often arises from sets of simple rules. We examined the contrast enhancement patterns MRI scans glioblastoma patients for evidence fractal correlated these with gene expression patterns. For 39 patients, volumetric T1 post-contrast were obtained before surgical resection. each tumor, we calculated dimension (Minkowski–Bouligand dimension) a box-counting (cubic scaling) approach. RNA microarray data resected tissue explored...
Abstract BACKGROUND Status Epilepticus (SE) is a potentially life-threatening and highly debilitating manifestation of diffuse glioma. Glioma-associated SE has yet been poorly studied; consequently, evidence-based recommendations for clinical management are scarce. Prevalence predictors might vary across the biomolecular heterogeneity We aimed to determine prevalence in grade 2 3 glioma throughout disease trajectory, identify associated baseline determinants (BD) short-term (STD). MATERIAL...