A5064361052- Brucella: diagnosis, epidemiology, treatment
- Galectins and Cancer Biology
- Burkholderia infections and melioidosis
- Monoclonal and Polyclonal Antibodies Research
- Mycobacterium research and diagnosis
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Salmonella and Campylobacter epidemiology
- Neuroinflammation and Neurodegeneration Mechanisms
- Inflammation biomarkers and pathways
- Multiple Sclerosis Research Studies
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Venomous Animal Envenomation and Studies
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Tuberculosis
- Healthcare and Venom Research
- Salivary Gland Disorders and Functions
- Protein Tyrosine Phosphatases
- Renal Transplantation Outcomes and Treatments
- HIV/AIDS oral health manifestations
- Inflammasome and immune disorders
- Animal Virus Infections Studies
- HIV Research and Treatment
- Apelin-related biomedical research
Universidad de Málaga
2007-2024
Hospital Regional Universitario de Málaga
2004-2024
Hospital La Paz Institute for Health Research
2016
Hospital Universitario Rey Juan Carlos
2011
Instituto de Salud Carlos III
2007
Hospital Doctor José Molina Orosa
2003
University of Chile
1993-1994
Real-time PCR is a widely used tool for the diagnosis of many infectious diseases. However, little information exists about influences different factors involved in on amplification efficiency. The aim this study was to analyze effect boiling as DNA preparation method efficiency process real-time human brucellosis with serum samples. Serum samples from 10 patients were analyzed by SYBR green I LightCycler-based and using obtain DNA. prepared lysis bacteria isolated did not prevent presence...
Background: Although not definitively proven, there is commonly accepted to be a latitudinal gradient in the distribution of multiple sclerosis (MS), which more frequent temperate zones. The European Mediterranean countries are situated zone median frequency, although ever increasing figures have been noted last decades. Objective: objective this study was assess current prevalence rate MS province Malaga, Southern Spain. Methods: capture–recapture method (CRM) uses independent sources data...
Case-control study for assessing a diagnostic test.The aim of this was to analyze the yield multiplex real-time polymerase chain reaction (PCR) assay in differential diagnosis tuberculous vertebral osteomyelitis (TVO) and brucellar (BVO).Vertebral (VO) is one commonest osteoarticular complications tuberculosis brucellosis. However, very similar clinical, radiologic, histologic characteristics these entities mean that requires etiological confirmation, but conventional microbiologic methods...
Summary We genotyped 83 patients with brucellosis and 101 controls to determine the influence of polymorphisms interferon gamma (IFNG) interleukin 10 (IL10) genes on protection against, or susceptibility to, human its complications. The results showed a significant increase in IFNG +874A/A genotype compared (34% vs. 19%) ( P = 0.023, odds ratio 2.17, 95% confidence interval 1.05–4.51). No differences were detected frequency distribution IL10 genotypes between controls. Similarly, no observed...
Background: Microglial dysfunction plays a causative role in Alzheimer’s disease (AD) pathogenesis. Here we focus on germline insertion/deletion variant mapping SIRPβ1, surface receptor that triggers amyloid-β(Aβ) phagocytosis via TYROBP. Objective: To analyze the impact of this copy-number SIRPβ1 expression and how it affects AD molecular etiology. Methods: Copy-number proxy rs2209313 was evaluated GERALD GR@ACE longitudinal series. Hippocampal specimens genotyped patients were also...
Background Arduous to differ clinically, extrapulmonary tuberculosis and focal complications of brucellosis remain important causes morbidity mortality in many countries. We developed applied a multiplex real-time PCR assay (M RT-PCR) for the simultaneous detection Mycobacterium complex Brucella spp. Methodology Conventional microbiological techniques M RT-PCR M. spp were performed on 45 clinical specimens from patients with or 26 control samples. Fragments 207 bp 164 conserved region genes...
Background Both brucellosis and tuberculosis are chronic-debilitating systemic granulomatous diseases with a high incidence in many countries Africa, Central South America, the Middle East Indian subcontinent. Certain focal complications of extrapulmonary very difficult to differentiate clinically, biologically radiologically. As conventional microbiological methods for diagnosis two have limitations, as well being time-consuming, multiplex real time PCR (M RT-PCR) could be promising...
The aim of this study was to investigate the possible influence tumor necrosis factor alpha (TNFA) gene promoter polymorphisms and HLA class II genes on susceptibility or development human brucellosis. TNFA genotypes (at positions -308 -238) were determined in 59 patients with brucellosis 160 healthy controls by polymerase chain reaction-restriction fragment length polymorphism. There no significant differences between for TNFA-238 genotypes. However, when overall TNFA-308 genotype...
Abstract We investigated the polymorphism of transmembrane region MICA gene (major histocompatibility complex class I chain‐related A) in relation to susceptibility human brucellosis. typed 114 patients with brucellosis and 121 healthy controls for polymerase chain reaction methods combined fluorescent technology. found a significant decrease frequency MICA‐A4 allele compared (4.4% vs 10.3%, P c = 0.03). The MICA‐A5 was increased group focal complications (15% 38%, 0.004). Our data suggest...
The PTPN22 gene encodes for an intracellular lymphoid-specific phosphatase (Lyp) that has a negative regulatory effect on T-cell activation. minor allele of the single nucleotide polymorphism (SNP) in encoding Lyp-tyrosine been associated with multiple autoimmune disorders and susceptibility to M. tuberculosis. It is possible, therefore, variants this may also be involved another pathogen, B. melitensis, which gives rise human brucellosis. Accordingly, we studied 111 patients brucellosis 150...
In an attempt to clarify the issue of genetic predisposition leprosy, we examined distribution class II human leucocyte antigen variants (DR and DQ) in 70 patients from around city Goiânia, Brazil. Only two presented tuberculoid form disease, whereas 17 fell into lepromatous category; 51 were intermediate. The allele frequencies found compared with those a group 77 healthy controls. We increased frequency HLA-DRB1*11 leprosy controls (P=0.0132; RR=4.130, 95% Cl: 1.338 12.747). These results...
Abstract Toll-like receptor 2 (TLR2) plays a key role in innate immune response recognizing molecular patterns expressed by pathogens. rs111200466 is TLR2 promoter insertion/deletion polymorphism with contradictory data about its human immunodeficiency virus type 1 (HIV-1) infection. We analyzed HIV-1 disease progression and showed correlation faster to the CD4+ < 200 cells/μL outcome for deletion allele carriers (Cox regression analysis: hazard ratio, 2.4 [95% confidence interval,...
Survival of children with combined immunodeficiency is strongly related to patient's age and clinical situation at the time hematopoietic stem cell transplantation (HSCT). We describe features before HSCT or enzyme replacement therapy (ERT) in a cohort treated National Reference Unit.A retrospective study CIDs our Hospital during 20-year period (1995-2014) was performed, analyzing their HSCT/ERT.Thirty-one were included. Risk factors such as family history consanguinity present 35% cases,...
It has been reported that specific killer-cell immunoglobulin-like receptors (KIRs) and HLA genotype combinations, such as KIR2DS4/HLA-C1 with presence of KIRDL2 or KIRDL3, homozygous KIRDL3/HLA-C1 KIR3DL1/≥2HLA-Bw4, are strongly associated the lack active infection seroconversion after exposition to hepatitis C virus (HCV).