A5101423860- Traditional Chinese Medicine Analysis
- Plant biochemistry and biosynthesis
- Microbial Natural Products and Biosynthesis
- Ginseng Biological Effects and Applications
- Metabolomics and Mass Spectrometry Studies
- Genomics and Phylogenetic Studies
- Medicinal Plant Pharmacodynamics Research
- Plant tissue culture and regeneration
- Berberine and alkaloids research
- Plant-based Medicinal Research
- Natural product bioactivities and synthesis
- Phytochemistry and Biological Activities
- Pharmacological Effects of Natural Compounds
- Plant Gene Expression Analysis
- Network Traffic and Congestion Control
- Advanced Queuing Theory Analysis
- Plant Disease Resistance and Genetics
- Remote Sensing in Agriculture
- Traditional and Medicinal Uses of Annonaceae
- Chromatography in Natural Products
- Plant Genetic and Mutation Studies
- Alkaloids: synthesis and pharmacology
- Remote Sensing and Land Use
- Microbial Metabolism and Applications
- Photochromic and Fluorescence Chemistry
Gansu University of Traditional Chinese Medicine
2023-2025
Yunnan Provincial Department of Education
2023-2025
Yunnan University of Traditional Chinese Medicine
2019-2025
Yunnan University
2010-2025
Tasly Holding Group (China)
2023
Lanzhou University
2022-2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2012-2019
Chengdu University of Traditional Chinese Medicine
2015-2016
University of Illinois Chicago
1994
Cytochromes P450 (CYPs) play a key role in generating the structural diversity of terpenoids, largest group plant natural products. However, functional characterization CYPs has been challenging because expansive families found genomes, diverse reactivity and inaccessibility their substrates Here we present two CYPs, CYP76AH3 CYP76AK1, which act sequentially to form bifurcating pathway for biosynthesis tanshinones, oxygenated diterpenoids from Chinese medicinal Danshen (Salvia miltiorrhiza)....
Abstract Tanshinones are the bioactive nor -diterpenoid constituents of Chinese medicinal herb Danshen ( Salvia miltiorrhiza ). These groups chemicals have characteristic furan D-ring, which differentiates them from phenolic abietane-type diterpenoids frequently found in Lamiaceae family. However, how 14,16-epoxy is formed has not been elucidated. Here, we report an improved genome assembly using a highly homozygous genotype. We identify cytochrome P450 (CYP71D) tandem gene array through...
The crosstalk between ferroptosis and cancer metastasis remains unclear. Here, we identify AMER1 as a key regulator of ferroptosis. loss causes resistance to in colorectal (CRC) cells. Interestingly, AMER1-deficient CRC cells preferentially form distant metastases, while AMER1-naive mainly invade lymph nodes. Moreover, the inhibitor liproxstatin-1 effectively promotes hematogenous transfer Mechanistically, binds SLC7A11 ferritin light chain (FTL) recruits β-TrCP1/2, which degrade FTL by...
The medicinal plant Stephania yunnanensis is rich in aporphine alkaloids, a type of benzylisoquinoline alkaloid (BIA), with being the representative and most abundant compound, but our understanding biosynthesis BIAs this has been relatively limited. Previous research reported genome S. preliminarily identified norcoclaurine synthase (NCS), which involved early stages BIA biosynthetic pathways. However, key genes promoting formation skeleton have not yet reported. In study, based on...
Background/Objectives: Stephania epigaea is a plant from the Menispermaceae family. Its root an important traditional folk medicine, which called Diburong in China. rich benzylisoquinoline alkaloids (BIAs), including cepharanthine, has been demonstrated to exhibit significant anti-inflammatory, antiviral, antineoplastic, and anti-SARS-CoV-2 activities, as well raising leukocytes. Cepharanthine composed of (R)- (S)-1-benzylisoquinoline alkaloid (1-BIA). (S)-norcoclaurine-6-O-methyltransferase...
The dry root and rhizome of Salvia miltiorrhiza Bunge, or Danshen, is a well-known, traditional Chinese medicine. Tanshinones are active compounds that accumulate in the periderm, resulting red-colored roots. However, lines with orange roots have been observed cultivated fields. Here, we performed metabolome transcriptome analyses to investigate changes orange-rooted Danshen.Metabolome analysis was by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry...
Cytochrome P450s (CYPs) are important enzymes in the secondary metabolism of plants and have been recognized as key players bioengineering synthetic biology. Previously reported CYP76AH1 CYP76AH3, having greater than 80% sequence homology, played a continuous catalytic role biosynthesis tanshinones Salvia miltiorrhiza. Homology modeling indicates that four sites might be responsible for differences activity between two enzymes. A series modeling-based mutational variants were designed to...
Abstract The genetic bases of halophytes for salinity tolerance are crucial genetically breeding salt-tolerant crops. All natural Nitrariaceae species that exclusively occur in arid environments highly tolerant to salt stress, but the underlying genomic this adaptation remain unknown. Here we present a high-quality, chromosome-level genome sequence Nitraria sibirica, with an assembled size 456.66 Mb and 23,365 annotated genes. Phylogenomic analyses confirmed N. sibirica as sister all other...
Diterpenoid alkaloids (DAs) are major pharmacologically active ingredients of Aconitum vilmorinianum, an important medicinal plant. Cytochrome P450 monooxygenases (P450s) involved in the DA biosynthetic pathway, and electron transfer reaction NADPH-cytochrome reductase (CPR) with is rate-limiting step redox reaction. Here, we identified characterized two homologs CPR from vilmorinianum. The open reading frames AvCPR1 AvCPR2 were found to be 2103 2100 bp, encoding 700 699 amino acid residues,...
Elaeagnus moorcroftii Wall. (Elaeagnaceae) is an important tree species naturally growing in arid Northwest China that has great economic and ecological values drylands. In this study, we de novo assembled a chromosome-level genome for E. by using PacBio’s high-fidelity (HiFi) sequencing Hi-C-assisted assembly technology. The size was 529.56 Mb, of which 94.56% anchored to 14 pseudochromosomes with contig N50 up 28.21 Mb. total, 29,243 protein-coding genes were annotated, 98.5% the...
Aconitum vilmorinianum is an authentic and superior medicinal herbal in Yunnan, which rich yunaconitine other diterpene alkaloids. Diterpene alkaloids are its main active components. Farnesyl pyrophosphate synthase(FPS) a key enzyme the terpene biosynthetic pathway plays important role alkaloid biosynthesis. Functional studies of FPS help to reveal molecular mechanism In this study, one gene(AvFPS) was selected based on transcriptome data A. vilmorinianum. Its full-length sequence cloned,...
Tunicosaponins are natural products extracted from Psammosilene tunicoides, which is an important ingredient of Yunnan Baiyao Powder, ancient and famous Asian herbal medicine. The representative aglycones tunicosaponins the oleanane-type triterpenoids gypsogenin quillaic acid, were found to manipulate a broad range virus–host fusion via wrapping heptad repeat-2 (HR2) domain prevalent in viral envelopes. However, unknown biosynthetic pathway difficulty chemical synthesis hinder therapeutic...
Squalene synthase (SQS) is the key precursor in synthesis of cholesterol. Located downstream relation to hydroxy methylglutaryl coenzyme A reductase and having no influence on formation biologically necessary isoprenoids make it an interesting target for development cholesterol lowering drugs with fewer side effects. To discover novel SQS inhibitors, three‐dimensional quantitative structure‐activity relationship pharmacophore models were built further validated by cost function analysis,...
This paper proposed a novel analytical model of the polling MAC protocol using M-gated services in continuous time. By imbedded Markov chain theory, generating functions queue length at server arrival epoch is obtained. Also, Laplace-Stieltjes transform (LST) packet delay distribution function derived. Then computational equations are explicitl determined for mean and delay. Through analysis mechanism model, its control values service related properties have been Meanwhile, basic systems...
Screening suitable reference genes is the premise of quantitative Real-time PCR(qRT-PCR)for gene expression analysis. To provide stable for analysis in Aconitum vilmorinianum, this study selected 19 candidate re-ference genes(ACT1, ACT2, ACT3, aTUB1, aTUB2, bTUB, 18S rRNA, UBQ, eIF2, eIF3, eIF4, eIF5, CYP, GAPDH1, GAPDH2, PP2A1, PP2A2, ACP, and EF1α) based on transcriptome data A. vilmorinianum. qRT-PCR was conducted to profile these root, stem, leaf, flower The Ct values showed that rRNA...
<title>Abstract</title> The medicinal plant <italic>Stephania yunnanensis</italic> is rich in aporphine alkaloids, a type of benzylisoquinoline alkaloids (BIAs), with being the representative and most abundant compound, but our understanding on biosynthesis BIA this have been relatively limited. Previous research has reported genome <italic>S. preliminarily identified upstream gene norcoclaurine synthase (NCS) biosynthetic pathways. However, key genes promoting formation skeleton not yet...
<title>Abstract</title> <italic>Gentiana macrophylla</italic> Pall. is a medicinally important perennial herb used in traditional Chinese and ethnic medicines. Iridoids are the main chemical constituents of <italic>G. macrophylla.</italic> The scarcity wild resource has led to increased attention cultivated macrophylla</italic>. However, little known about metabolic differences regulatory mechanisms between This study utilized untargeted metabolomics transcriptomics reveal accumulated...