A5079545643- Salmonella and Campylobacter epidemiology
- Systemic Lupus Erythematosus Research
- Cryospheric studies and observations
- Bacterial Identification and Susceptibility Testing
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related gene regulation
- Climate change and permafrost
- Viral gastroenteritis research and epidemiology
- Kruppel-like factors research
- Social and Educational Sciences
- Bacteriophages and microbial interactions
- T-cell and B-cell Immunology
- Streptococcal Infections and Treatments
- Meteorological Phenomena and Simulations
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Protein Tyrosine Phosphatases
- Health, psychology, and well-being
- Molecular Biology Techniques and Applications
- Antibiotics Pharmacokinetics and Efficacy
- Geology and Paleoclimatology Research
- Climate variability and models
- Vibrio bacteria research studies
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
Gradientech (Sweden)
2021-2024
Uppsala University
2006-2021
Stockholm University
2020
Science for Life Laboratory
2016
The rapidly changing landscape of antimicrobial resistance poses a challenge for empirical antibiotic therapy in severely ill patients and highlights the need fast susceptibility diagnostics to guide treatment. Traditional methods testing (AST) bacteria such as broth microdilution (BMD) or disc diffusion method (DDM) are comparatively slow show high variability. Rapid AST under development often trade speed resolution, sometimes only measuring responses at single concentration. QuickMIC is...
ABSTRACT Common phenotypic methods for antimicrobial susceptibility testing (AST) of bacteria are slow, labor intensive, and display considerable technical variability. The QuickMIC system provides rapid AST using a microfluidic linear gradient. Here, we evaluate the performance at four different laboratories with regard to speed, precision, accuracy, reproducibility in comparison broth microdilution (BMD). Spiked ( n = 411) clinical blood cultures 148) were tested Gram-negative panel...
To further analyze a clinical isolate originally identified as methicillin-resistant Staphylococcus aureus (MRSA) using whole-genome sequencing and comparative genomics.Classical diagnostic methods such cultivation, biochemical tests, PCR were supplemented with genomics, to identify the isolate.The was phenotypically similar MRSA. However, presence of nuc gene could not be confirmed PCR, while it positive for mecA gene. Whole-genome correctly argenteus. The possessed several resistance...
The role of water for transmission Campylobacter jejuni and C. coli to humans might be underestimated, as factors important bacterial viability in are largely unknown. We have studied survival seven eight isolates originally isolated from Swedish waters, together with selected reference strains, over days at 4 °C the dark untreated collected a local lake private well. To study seasonality, samples were during spring autumn. Samples culturable counts taken on 2, 4, 6, 8 compared start...
Staphylococcus argenteus and schweitzeri are newly identified species of the S. aureus-related complex. argenteus, as occurring globally showing significant prevalence comparable infection morbidity rates compared to aureus, is becoming clinically important. Whole genome sequencing has revealed presence several virulence genes but molecular mechanisms largely unknown. Here, we studied effect a previously characterized clinical isolate on human cells in vitro. The isolate, together with type...
Campylobacter jejuni is an important foodborne pathogen and the most commonly reported bacterial cause of gastroenteritis. C. occasionally found in blood, although mechanisms for invasiveness have remained unclear. divided into many different lineages, which ST21 clonal complex (CC) widely distributed. Here, we performed comparative genomic vitro analyses on 17C. ST21CC strains derived from human blood feces order to identify features associated with isolation site. The lineage two large...
ABSTRACT Whole transcriptome sequencing (RNA-seq) has become a standard for cataloguing and monitoring RNA populations. Among the plethora of reconstructed transcripts, one main bottlenecks consists in correctly identifying different classes RNAs, particularly those that will be translated (mRNAs) from class long non-coding RNAs (lncRNAs). Here, we present FEELnc (FlExible Extraction LncRNAs), an alignment-free program which accurately annotates lncRNAs based on Random Forest model trained...
Myosin regulatory light chain interacting protein (MIR) belongs to the ezrin, radixin, moesin (ERM) family of proteins involved in membrane cytoskeleton interactions and cell dynamics. MIR contains, beside ERM domain, a RING zinc finger region. Immunocytochemistry showed that full‐length subdomains localize differently cells. Cell fractionation revealed similar distribution domain Triton X‐100‐insoluble fraction. The neurite outgrowth inhibitory activity was attributed domain. levels were...
Campylobacter jejuni is the most common cause of bacterial gastroenteritis. Major reservoirs are warm-blooded animals, poultry in particular, but can also be transmitted via water. In this paper, we have taken a closer look at biology and potential virulence C. water isolates. Seven isolates from incoming surface plants Sweden were characterized with whole genome sequencing phenotypical testing. Multi locus sequence typing analysis revealed that these belonged to groups known include both...
Snow particle size is an important parameter strongly affecting snow cover broadband albedo from seasonally covered areas and ice sheets. It also in remote sensing analyses because it influences the reflectance scattering properties of snow. We have developed a digital image processing method for capture analysis data shape. The suitable quick reliable field. Traditional methods based on visual inspection samples been used but do not yield quantitative data. Our provides alternative to both...
Abstract Campylobacter jejuni and coli are important bacterial enteropathogens. Poultry is the best‐known reservoir for infection but natural bodies of water have also been shown to be pathways transmission. can survive in cold most studies focused on C . only. In this paper, we take a closer look at biology survival strategies Eight isolates cultivated from raw (incoming) surface plants Sweden were characterized using whole‐genome sequencing phenotypical assays. Phylogenetic analysis...
Campylobacter colonise the gastrointestinal tract of warm-blooded animals and are major enteropathogens in humans. C. coli is less common than jejuni accounts for about 10% total number infections although two species seem to share many virulence determinants. bacteraemia rare, estimated occur 1% infections, exact mechanisms regulating progression infection from blood stream unclear. Here, we looked at contribution further compared various traits clade 1 stool isolates. We assessed numbers...
Campylobacter bacteria are major human enteropathogens. coli shows less genetic diversity than C. jejuni and clusters into three clades, of which clade 1 includes most farm animal isolates, while environmental isolates mainly belong to clades 2 3. Recently, we sequenced the whole genomes eight 3 cultivated from water, here studied their interaction with HT-29 colon cancer cells compared that clinical isolates. All already caused cell necrosis h after inoculation, whereas none analyzed...
Campylobacter infections are the leading cause of bacterial gastroenteritis. In Europe, over 246,000 cases confirmed annually. Infections often transmitted via contaminated food, such as poultry products, but water may be source infection well. The aim this study was to characterize a selection jejuni human isolates, together with isolate, from waterborne outbreak in Norway 2019, including isolates early, mid- and late epidemic. were characterized whole-genome sequencing, analysing...
Abstract Objectives Common phenotypic methods for antibiotic susceptibility testing (AST) of bacteria are slow, labour intensive and display considerable technical variability. The QuickMIC system provides rapid AST using a microfluidic linear gradient. Here we evaluate the performance at four different laboratories with regards to speed, precision, accuracy, reproducibility in comparison broth microdilution (BMD). Methods Spiked blood cultures (n=411) clinical (n=148) were tested Gram...
Corresponding author address: Dr. Ulf Högström, Department of Earth Sciences, Meteorology, University Uppsala, Villavägen 16, Uppsala S-75236, Sweden. Email: ulf.hogstrom@met.uu.se