A5102731839- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- MicroRNA in disease regulation
- Plant Pathogens and Resistance
- Circular RNAs in diseases
- Genetic Mapping and Diversity in Plants and Animals
- Toxoplasma gondii Research Studies
- Microbial infections and disease research
- Syphilis Diagnosis and Treatment
- TGF-β signaling in diseases
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Plant Reproductive Biology
- Metabolism, Diabetes, and Cancer
- Antibiotic Resistance in Bacteria
- Genomics and Chromatin Dynamics
- Phytochemistry and Biological Activities
- Muscle Physiology and Disorders
- Photosynthetic Processes and Mechanisms
- Extracellular vesicles in disease
- Phytochemical Studies and Bioactivities
University of South China
2024
Beijing Chest Hospital
2023
Capital Medical University
2020-2023
BioNano Genomics (United States)
2016-2021
Nantong University
2020
Baotou Medical College
2020
Taizhou People's Hospital
2020
Taian City Central Hospital
2019
University of Georgia
2009-2014
University of California, Riverside
2011
Although draft genomes are available for most agronomically important plant species, the majority incomplete, highly fragmented, and often riddled with assembly scaffolding errors. These issues hinder advances in tool development functional genomics systems biology.Here we utilized a robust, cost-effective approach to produce high-quality reference genomes. We report near-complete genome of diploid woodland strawberry (Fragaria vesca) using single-molecule real-time sequencing from Pacific...
The maize W22 inbred has served as a platform for genetics since the mid twentieth century. To streamline genome analyses, we have sequenced and de novo assembled reference using short-read sequencing technologies. We show that significant structural heterogeneity exists in comparison to B73 at multiple scales, from transposon composition copy number variation single-nucleotide polymorphisms. generation of this enables accurate placement thousands Mutator (Mu) Dissociation (Ds) transposable...
We describe a comprehensive and general approach for mapping centromeres present detailed characterization of two maize centromeres. Centromeres are difficult to map analyze because they consist primarily repetitive DNA sequences, which in the tandem satellite repeat CentC interspersed centromeric retrotransposons (CRM). defined epigenetically by histone H3 variant, CENH3. Using novel markers derived from centromere repeats, we have mapped all ten onto physical genetic maps maize. were able...
Biolistic transformation delivers nucleic acids into plant cells by bombarding the with microprojectiles, which are micron-scale, typically gold particles. Despite wide use of this technique, little is known about its effect on cell's genome. We biolistically transformed linear 48-kb phage lambda and two different circular plasmids rice (Oryza sativa) maize (Zea mays) analyzed results whole genome sequencing optical mapping. Although some transgenic events showed simple insertions, others...
Centromeres are the most dynamic regions of genome, yet they typified by little or no crossing over, making it difficult to explain origin this diversity. To address question, we developed a novel CENH3 ChIP display method that maps kinetochore footprints over transposon-rich areas centromere cores. A high level polymorphism made possible map total 238 within-centromere markers using maize recombinant inbred lines. Over half were shown interact directly with kinetochores (CENH3) chromatin...
Significance B chromosomes are nonvital found in thousands of plants and animals that persist through various drive mechanisms. The mechanism the maize chromosome consists mitotic nondisjunction at second pollen division to produce two unequal sperm then with preferentially fertilizes egg double fertilization. A high-quality sequence together genetic analysis reveals cis factor for is a chromosome-specific repeat interspersed around centromere. gene transposable element content relaxed...
Abstract We report a detailed analysis of maize chromosome structure with respect to seven histone H3 methylation states (dimethylation at lysine 4 and mono-, di-, trimethylation lysines 9 27). Three-dimensional light microscopy the fine cytological resolution pachytene chromosomes made it possible compare distribution individual events each other DNA staining intensity. Major conclusions are that (1) H3K27me2 marks classical heterochromatin; (2) H3K4me2 is limited areas between around...
ABSTRACT Complete and accurate reference genomes annotations provide fundamental tools for characterization of genetic functional variation. These resources facilitate elucidation biological processes support translation research findings into improved sustainable agricultural technologies. Many crop plants have been generated over the past decade, but these are often fragmented missing complex repeat regions. Here, we report assembly annotation maize, a model species, using Single Molecule...
Circular RNA (circRNA) is highly expressed in the brain tissue, but its molecular mechanism cerebral ischemia–reperfusion remains unclear. Here, we explored role and underlying mechanisms of circRNA antisense non-coding INK4 locus (circ_ANRIL) oxygen–glucose deprivation reoxygenation (OGD/R)-induced cell injury. The expression circ_ANRIL OGD/R-induced human microvascular endothelial cells (HBMECs) was significantly up-regulated, while that miR-622 down-regulated. Overexpression inhibited...
Ischemic tolerance in the brain can be induced by transient limb ischemia, and this phenomenon is termed remote ischemic preconditioning (RIPC). It still remains elusive how transfer of occurs. Exosomes cross blood-brain barrier, some molecules may neuroprotective signals from periphery to brain. Serum miRNA-126 associated with stroke, exosomal has shown protective effects against acute myocardial infarction. Therefore, study aims explore whether RIPC serum play a similar role. were isolated...
Bedaquiline (BDQ) is an effective drug for the treatment of drug-resistant tuberculosis. Mutations in atpE , which encodes target BDQ, are associated with large increases MICs. Rv0678 that derepress transcription MmpL5-MmpS5 efflux transporter smaller However, mutations most common BDQ resistance clinical isolates, and they also confer cross-resistance to clofazimine (CFZ).
ABSTRACT We biolistically transformed linear 48 kb phage lambda and two different circular plasmids into rice maize analyzed the results by whole genome sequencing optical mapping. While some transgenic events showed simple insertions, others extreme damage in form of chromosome truncations, large deletions, partial trisomy, evidence chromothripsis breakage-fusion bridge cycling. Several contained megabase-scale arrays introduced DNA mixed with genomic fragments assembled non-homologous or...
Abstract Centromere repeats are found in most eukaryotes and play a critical role kinetochore formation. Though CentC exhibit considerable diversity both within among species, little is understood about the mechanisms that drive centromere repeat evolution. Here, we use maize as model to investigate how complex history involving polyploidy, fractionation, recent domestication has impacted of repeat. We first validate existence long tandem arrays other taxa genus Zea . Although find sequence...
In order to investigate the antitumor effects of thein vivo G-CSF gene therapy mediated by liposome and its mechanisms, human was encapsulated into directly injected tumor mass C-26 colon adenocardnoma-bearing mice. After direct intratumoral injection DNA, subcutaneous growth dramatically inhibited survival time prolonged significantly. Tumor regression could be observed in about 30% C-26-bearing By analysis we found that anti-G41s (600ug/ml) clone selected from cells freshly separated...