A5057818762- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Immune cells in cancer
- S100 Proteins and Annexins
- Neuroinflammation and Neurodegeneration Mechanisms
- Periodontal Regeneration and Treatments
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Wound Healing and Treatments
- Bioeconomy and Sustainability Development
- Immune Response and Inflammation
- Fibroblast Growth Factor Research
- Agronomic Practices and Intercropping Systems
- Kawasaki Disease and Coronary Complications
- Liver Diseases and Immunity
- Cancer Immunotherapy and Biomarkers
- interferon and immune responses
- COVID-19 Clinical Research Studies
- Hepatitis B Virus Studies
- Plant Taxonomy and Phylogenetics
- Nonmelanoma Skin Cancer Studies
- COVID-19 Impact on Reproduction
Ghent University
2020-2024
VIB-UGent Center for Inflammation Research
2020-2024
VIB-VUB Center for Structural Biology
2022-2024
VIB Single Cell Core
2022-2024
VIB-KU Leuven Center for Cancer Biology
2023-2024
In rare instances, pediatric SARS-CoV-2 infection results in a novel immunodysregulation syndrome termed multisystem inflammatory children (MIS-C). We compared MIS-C immunopathology with severe COVID-19 adults. does not result pneumocyte damage but is associated vascular endotheliitis and gastrointestinal epithelial injury. MIS-C, the cytokine release characterized by IFNγ type I interferon. Persistence of patrolling monocytes differentiates from COVID-19, which dominated HLA-DRlo classical...
Long considered to fluctuate between pro- and anti-inflammatory states, it has now become evident that microglia occupy a variegated phenotypic landscape with relevance aging neurodegeneration. However, whether specific microglial subsets converge in or contribute both processes eventually affect brain function is less clear. To investigate this, we analyzed heterogeneity tauopathy mouse model (K18-seeded P301L) an accelerated (Senescence-Accelerated Mouse-Prone 8, SAMP8) using cellular...
Inflammatory signaling pathways are tightly regulated to avoid chronic inflammation and the development of disease. OTULIN is a deubiquitinating enzyme that controls by cleaving linear ubiquitin chains generated chain assembly complex. Here, we show ablation in liver parenchymal cells mice causes severe disease which characterized inflammation, hepatocyte apoptosis, compensatory proliferation, leading steatohepatitis, fibrosis, hepatocellular carcinoma (HCC). Genetic Fas-associated death...
Abstract OTULIN is a deubiquitinase that specifically cleaves linear ubiquitin chains. Here we demonstrate the ablation of Otulin selectively in keratinocytes causes inflammatory skin lesions develop into verrucous carcinomas. Genetic deletion Tnfr1 , knockin expression kinase-inactive Ripk1 or keratinocyte-specific Fadd and Mlkl completely rescues mice with deficiency from dermatitis tumorigenesis, thereby identifying keratinocyte cell death as driving force for inflammation. Single-cell...
In the human thymus, a CD10+ PD-1+ TCRαβ+ differentiation pathway diverges from conventional single positive T cell lineages at early double-positive stage. Here, we identify progeny of this unconventional lineage in antigen-inexperienced blood. These cells (UTCs) thymus and blood share transcriptomic profile, characterized by hallmark transcription factors (i.e., ZNF683 IKZF2), polyclonal TCR repertoire with autoreactive features, exhibiting bias toward TCRα chain rearrangements....
Lymphatic endothelial cells (LECs) of the lymph node (LN) parenchyma orchestrate leukocyte trafficking and peripheral T cell dynamics. responses to immunotherapy largely rely on recruitment in tumors. Yet, a systematic molecular understanding how LECs within LNs control dynamics under steady-state tumor-bearing conditions is lacking. Intravital imaging combined with immune phenotyping shows that LEC-specific deletion essential autophagy gene Atg5 alters intranodal positioning lymphocytes...
Macroautophagy/autophagy is a cellular recycling program regulating cell survival and controlling inflammatory responses in context-dependent manner. Here, we demonstrate that keratinocyte-selective ablation of Atg16l1, an essential autophagy mediator, results exacerbated neoplastic skin responses. In addition, mice lacking keratinocyte exhibit precocious onset hair follicle growth, indicating altered activation kinetics stem cells (HFSCs). These HFSCs also expanded potencies...
Abstract Single-cell RNA sequencing is instrumental to unravel the cellular and transcriptomic heterogeneity of T B cells in health disease. Recent technological advances add additional layers information allowing researchers simultaneously explore transcriptomic, surface protein immune receptor diversity during adaptive responses. The increasing data complexity poses a burden on workload for bioinformaticians, who are often not familiar with specificities biology profiling. wet-lab...
Abstract Despite major improvements in immunotherapeutic strategies, the immunosuppressive tumor microenvironment remains a obstacle for induction of efficient antitumor responses. In this study, we show that local delivery bispecific Clec9A-PD-L1 targeted type I interferon (AcTaferon, AFN) overcomes hurdle by reshaping immune landscape. Treatment with AFN resulted presence pro-immunogenic tumor-associated macrophages and neutrophils, increased motility maturation profile cDC1 inflammatory...
Abstract In the human thymus, a CD10 + PD-1 TCRαβ differentiation pathway diverges from conventional single positive T cell lineages at early double stage. These cells are phenotypically and functionally similar to murine unconventional intraepithelial lymphocyte (uIEL) precursors. Here, progeny of uIEL lineage was identified in antigen-inexperienced blood. The uIELs thymus peripheral blood share transcriptomic profile, characterized by hallmark transcription factors (i.e. ZNF683 IKZF2 ),...
In vitro cultures remain crucial for studying the fundamental mechanisms of human T-cell development. Here, we introduce a novel in cultivation system based on ThymoSpheres (TS): dense spheroids consisting DLL4-expressing stromal cells and hematopoietic precursor cells, absence thymic epithelial cells. These are subsequently cultured at air-liquid interphase. TS generate large numbers mature T easy to manipulate, scalable, can be repeatably sampled monitor differentiation. The generated from...
Abstract Long considered to fluctuate between pro- and anti-inflammatory states, it has now become evident that microglia occupy a variegated phenotypic landscape with relevance aging neurodegeneration. However, whether specific microglial subsets converge in or contribute both processes eventually affect brain function is less clear. To investigate this, we analyzed heterogeneity tauopathy mouse model (K18-seeded P301L) an accelerated (senescence prone 8, SAMP8) using cellular indexing of...
ABSTRACT Lymphatic endothelial cells (LECs) lining the lymphatic vessels of lymph node (LN) parenchyma orchestrate leukocyte trafficking and peripheral T cell dynamics. responses to immunotherapy largely rely on recruitment in tumors. Yet, a systematic molecular understanding how LECs within LNs control dynamics under steady state tumor-bearing conditions is lacking. Using intravital high-resolution imaging combined with immune phenotyping, we show that LEC-specific deletion essential...
Background: ETP-ALL is a subtype of T cell acute lymphoblastic leukemia (T-ALL) and can be identified by characteristic immunophenotype gene expression profile. Treatment relapsed remains challenging using conventional chemotherapy little targeted therapies are available. A better understanding in the molecular mechanisms involved leukemogenesis maintenance could lead to improved more treatments. An estimated 9% patients carry mutations GATA3, transcription factor known regulate commitment...