Deep generative models are attracting great attention as a new promising approach for molecular design. A variety of reported so far based on either variational autoencoder (VAE) or adversarial network (GAN), but they have limitations such low validity and uniqueness. Here, we propose type model an adversarially regularized (ARAE). It basically uses latent variables like VAE, the distribution is estimated by training in GAN. The latter intended to avoid both insufficiently flexible...

Molecular structure-property relationships are key to molecular engineering for materials and drug discovery. The rise of deep learning offers a new viable solution elucidate the directly from chemical data. Here we show that performance graph convolutional networks (GCNs) prediction properties can be improved by incorporating attention gate mechanisms. mechanism enables GCN identify atoms in different environments. gated skip-connection further improves updating feature maps at an...

8-Cl-cyclic adenosine monophosphate (8-Cl-cAMP) has been known to induce growth inhibition and differentiation in a variety of cancer cells by differential modulation protein kinase A isozymes. To understand the anticancer activity 8-Cl-cAMP further, we investigated effect on apoptosis human cells. Most tested exhibited upon treatment with 8-Cl-cAMP, albeit different sensitivity. Among them, SH-SY5Y neuroblastoma HL60 leukemic showed most extensive apoptosis. The was not reproduced other...

Abstract For protein structure modeling in the CASP12 experiment, we have developed a new protocol based on our previous CASP11 approach. The global optimization method of conformational space annealing (CSA) was applied to 3 stages modeling: multiple sequence‐structure alignment, three‐dimensional (3D) chain building, and side‐chain re‐modeling. better template selection model selection, updated quality assessment (QA) with newly SVMQA (support vector machine for assessment). 3D energy...

Domain boundary prediction is one of the most important problems in study protein structure and function. Many sequence-based domain methods are either template-based or machine learning (ML) based. ML-based often perform poorly due to their use only local (i.e. short-range) features. These conventional features such as sequence profiles, secondary structures solvent accessibilities typically restricted be within 20 residues candidate.To address performance methods, we developed a new method...

The expression of the maltase (MALS) and maltose permease (MALT) genes in Saccharomyces species is coregulated at transcriptional level; they are coordinately induced by presence a positively acting regulatory (MALR) gene carbon catabolite repressed glucose. We generated series deletions upstream region MAL6S to examine elements detail. results showed that inducible was lost when between 320 380 base pairs translation initiation codon deleted. This contained an imperfect inverted repeat...

There is an enormous interest in renormalization of quasi-particle (qp) dispersion relation cuprate superconductors both below and above the critical temperature $T_c$ because it enables determination fluctuation spectrum to which qps are coupled. A remarkable discovery by angle-resolved photoemission spectroscopy (ARPES) a sharp low energy feature (LEF) qp spectra well superconducting gap but with its increasing proportion intensity sharply $T_c$. This unexpected needs be reconciled...

The Schizosaccharomyces pombe uvi31+ gene has been previously isolated as a UV-inducible [Lee JK et al. (1994) Biochem Biophys Res Commun 202:1113–1119]. This encodes protein of about 12 kDa with 57% amino acid sequence similarity to Escherichia coli BolA which is known be involved in switching between the cell elongation and septation systems during division cycle. putative Mlul cycle box (MCB), SWI4/6-dependent (SCB), gear-box elements are found upstream region gene, suggesting that this...

In CASP12, 2 types of data-assisted protein structure modeling were experimented. Either SAXS experimental data or cross-linking was provided for a selected number CASP12 targets that the predictor could utilize better modeling. We devised separate energy terms and to drive model structures into more native-like satisfied given as much possible. CASP11, we successfully performed using simulated sparse ambiguously assigned NOE and/or correct residue-residue contact information, where only...

We investigated the characteristics of spin fluctuations mediated superconductivity employing Eliashberg formalism. The effective interaction between electrons was modeled in terms susceptibility measured by inelastic neutron scattering experiments on single crystal La2-xSrxCuO4 superconductors. diagonal self-energy and off-diagonal were calculated solving coupled equation self-consistently for chosen tight-binding dispersion electrons. full momentum frequency dependence is presented...

To investigate the role of protein kinase A (PKA) (EC 2.7.1.37) in myogenesis, PKA activity was closely monitored during differentiation L6 rat skeletal myoblasts. As proceeded, total increased about 2–3 fold, and levels RIα Cα subunits 3–4 fold. We then looked at effect specific inhibitor for PKA, N-[2-(p-bromocinnamy-lamino)-ethyl]-5-isoquinoline-sulfonamide (H89), on H89 completely blocked myotube formation abolished up-regulation Cα. This inhibitory dose-dependent could be reversed upon...

도시의 발전 및 성장으로 인해 건물은 고층화, 대형화, 복잡화 되고 있으며, 효율적인 공간정보의 활용 공유를 위해 실내외 GIS의 중요성은 증가되고 있다. 하지만 도면 생성기술은 지형 2차원 3차원 생성에 대해서 주로 선행되었으며, 건물 실내공간의 구축 기술에 대한 연구는 미비한 실정이다. 본 연구에서는 지상 라이다로부터 취득된 실내 점군데이터를 이용한 반자동 기법을 제안하였다. 제안한 기법은 전처리, 도면생성, 도면생성 단계로 이루어진다. 전처리 단계는 공간의 높이를 측정하BR고 점군데이터의 노이즈를 식별한다. 생성 단계에서는 외곽선 추출격자와 정제과정을 이용하여 평면도를 생성한다. 과정에서 측정된 높이와 와이어프레임 모델을 식별된 노이즈 데이터는 와이어 프레임 모델과 함께 도면의 세부 모델링에 이용된다. 복도를 측량한 점군데이터에 적용하여 결과를 확인하였으며, 향후 GIS 구축을 위한 활용될 수 있을 것으로 기대된다.

Agmatine, an endogenous polyamine and putative neuromodulator, is known to have neuroprotective effects on various neurons in the central nervous system. We determined whether or not topically administered agmatine could reduce ischemic retinal injury. Transient ocular ischemia was achieved by intraluminal occlusion of middle cerebral artery ddY mice (30-35 g) for 2 h, which also induce ophthalmic artery. In group (N = 6), a 1.0 mM agmatine-containing solution four times daily weeks before...

Chimeric genes coding for prepro region of yeast α-factor and anglerfish SRIF were expressed in rat GH3 cells to determine whether signals could regulate hormone processing mammalian cells. We report that nascent hybrid polypeptides efficiently targeted ER, where cleavage signal peptides core glycosylation occurred, localized mainly Golgi. These data indicate functions sorting molecules secretory pathway A construct with a mutated peptide underwent similar ER translocation, whereas such...

We investigate the effects of dynamic bosonic fluctuations on Fermi surface reconstruction in two dimensions as a model for underdoped cuprates. At energies larger than boson energy $\omega_b$, nature is not important and quasi-particle dispersion exhibits shadow feature like that induced by static long range order. lower energies, however, pushed away finite $\omega_b$. The detailed low features are determined bare coupling quasi-particles to fluctuations. present how these factors...

SAR 위성 영상의 기하 위치정확도는 크게 시스템 내적 변수인 궤도 및 센서 정보와 외적 DEM 오차, 대기 지연 등에 영향을 받는다. 이러한 변수들이 현재 운용 중인 다목적실용위성 5호와 개발 6호의 영상 내 위치정확도에 미치는 분석하기 위하여 유사한 제원을 지닌 TerraSAR-X 영상을 이용하여 연구를 수행하였다. 오차 분석을 위해 경사거리 방정식과 도플러 방정식을 시뮬레이션 데이터를 생성하였으며, 변수, 변수에 대한 오차를 지상 좌표와 좌표 간 관계식에 반영하여 해당 변수들에 민감도 통해 저궤도 위성의 위치 속도 정보로 인한 오차는 이중주파수 GPS 데이터 이용에 따라 20cm 내외로 크지 않음이 확인되었다. 따라서 센서관련 줄 수 있으며, 특히 전자적 시간 지연은 방향의 정확도에 미쳐 지속적인 모니터링이 필요할 것으로 판단되었다. 변수 외에도 의한 오차로 인해 산지지역에서 Geocoding 시 오차가 20~30m까지 발생하고, 현상에 의해 입사각에 5m까지 발생할...

To investigate the mechanism of two forms messages (5.1 and 2.4 kb) in mouse Lamc2, a gene encoding for γ2 chain epithelial cell-specific laminin 5, we analyzed approximately 40 kb genomic DNA containing sequences from first intron to 23rd exon 3′ untranscribed region. With 5′ rapid amplification cDNA end (RACE) primer extension technique using RNA kidney thymic cells, found that smaller message starts at 2895th base within 19th exon. Upstream this alternative start site showed promoter...

Based on the surmise that a variety of genes might play important roles in embryonic development and tissue differentiation, some them are likely to be expressed undifferentiated ES cells, we attempted identify new from cell cDNA library. The modified method sequence tags (ESTs) examination expression patterns adult tissues vitro differentiated cells were utilized this study. We have isolated identified several novel clones with interesting developmental pattern. Among 83 randomly chosen, 23...

다중분광 위성영상을 이용한 원격탐측 기술은 광범위한 지역의 열섬효과 분석에 있어 유용하게 활용될 수 있다. 하지만 우리나라와 같이 구름이 많은 기상조건은 활용한 주기적인 관측을 어렵게 한다. 이에 본 연구에서는 시계열 Landsat 영상과 ATC 모델을 열섬현상 분석 방법을 제안하였다. 식생상태와 도시화정도를 분석하기 위하여 영상으로부터 NDVI와 NDBI를 산출하였으며 모델의 파라미터 추정을 열적외선 지표온도를 산출하여 활용하였다. 또한 토지 피복 및 이용형태에 따른 분석을 위해 환경부에서 제공하는 토지피복도를 기반으로 파라미터를 비교하였다. 산출한 분광지수와 간의 상관관계를 분석한 결과 MAST는 NDVI NDBI와 각각 -0.76, 0.69 의 강한 보였으며, YAST는 -0.53, 0.42의 나타냈다. 따라 비교한 도시 지역에서의 MAST와 YAST가 주변의 농업지역, 초지 등에 비해 높게 나타나는 것을 확인하였다. 지역 내에서 주거지역, 산업지역, 상업지역,...

As 3D geospatial information is demanded, terrestrial laser scanners which can obtain model of objects have been applied in various fields such as Building Information Modeling (BIM), structural analysis, and disaster management. To acquire precise data, performance evaluation a scanner must be conducted. While existing surveying equipment like total station has standard method for evaluation, technique users not established. This paper categorizes analyzes error sources generally occur...