A5004061274- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Rabies epidemiology and control
- Nanoplatforms for cancer theranostics
- Advanced Cellulose Research Studies
- Poxvirus research and outbreaks
- Material Properties and Processing
- Microbial infections and disease research
- Bone Tissue Engineering Materials
- biodegradable polymer synthesis and properties
- Viral Infections and Outbreaks Research
- Cancer Immunotherapy and Biomarkers
- HIV/AIDS drug development and treatment
- Nanoparticle-Based Drug Delivery
- Cancer Cells and Metastasis
- RNA Research and Splicing
- Liquid Crystal Research Advancements
- Advanced Theoretical and Applied Studies in Material Sciences and Geometry
- Metabolism and Genetic Disorders
- Cancer-related gene regulation
- Polymer crystallization and properties
- Graphene and Nanomaterials Applications
- Virology and Viral Diseases
- HIV Research and Treatment
- Epigenetics and DNA Methylation
Peking University First Hospital
2001-2023
Hainan University
2020-2023
Centers for Disease Control and Prevention
2019-2023
Peking University
2001-2023
OAI Consulting (United States)
2023
The Affiliated Shunde Hospital of Jinan University
2021
Jinan University
2018-2021
Materials Science & Engineering
2020
National Center for Emerging and Zoonotic Infectious Diseases
2019-2020
Haikou City People's Hospital
2020
Abstract During the 2022 multinational outbreak of monkeypox virus (MPXV) infection, antiviral drug tecovirimat (TPOXX; SIGA Technologies, Inc., https://www.siga.com) was deployed in United States on a large scale for first time. The MPXV F13L gene homologue encodes target tecovirimat, and single amino acid changes F13 are known to cause resistance tecovirimat. Genomic sequencing identified 11 mutations previously reported resistance, along with 13 novel mutations. Resistant phenotype...
Resistance to conventional chemotherapeutic agents, including irinotecan (CPT‑11), 5-fluorouracil and capecitabine is a major cause for therapeutic failure in patients with colorectal cancer (CRC). Increasing evidence has demonstrated that cells exhibiting stem cell-like characteristics are associated the development of resistance agents. As plant polyphenol, curcumin been have ability ameliorate CRC but associations among curcumin, (CSCs) chemoresistance remain unclear. The present study...
Utilizing nanoparticles to deliver subunit vaccines can be viewed as a promising strategy for enhancing the immune response, especially with regard cellular immunity fight against infectious viruses and malignant cancer. Nevertheless, its applications are still far from practicality because of some limitations such high cost, non-biocompatibility, non-biodegradability, inefficient stimulation cytotoxic T lymphocyte (CTL) response. In this study, we use metal-organic framework (MOF)...
Biodegradable blends of poly(butylene adipate-co-terephthalate) (PBAT) and stereocomplex polylactide (sc-PLA) were prepared herein via a melt blending method at various sc-PLA loadings. Wide-angle X-ray diffraction differential scanning calorimetry results verified that complete crystallites in the PBAT could be achieved. Scanning electron microscopy observation indicated was dispersed matrix as spherical particles; size did not display pronounced increase once content sc-crystallites...
Inherent and acquired chemoresistance reduce the effectiveness of irinotecan in treatment metastatic colorectal cancer (CRC). However, molecular mechanisms underlying this resistance process are still unclear. Twist1 is one master transcription factors epithelial-mesenchymal transition (EMT). Our previous study indicated that overexpressed colon tissues, demonstrated plays a crucial role CRC. In present study, we further investigated how contribute to cancer. The irinotecan-resistant cells...
In immunotherapy, induction of potent cellular immunity by vaccination is essential to treat intracellular infectious diseases and tumors. this work, we designed a new synthetic peptide carrier, Cys-Trp-Trp-Arg8-Cys-Arg8-Cys-Arg8-Cys, for vaccine delivery integrating redox-responsive disulfide bond cross-linking cell-penetrating arginine octamer. The carrier bound the antigen protein ovalbumin (OVA) via electrostatic self-assembly form peptide/OVA nanocomposites. Then, spontaneous...
Cancer nanovaccines have been widely explored to enhance immunotherapy efficiency, in which the significant irritation of antigen-specific cytotoxic T cells (CTLs) is critical point. In this study, we developed a pH and reduction dual-sensitive nanovaccine (PMSN@OVA-MPN) composed two parts. The inner part was made up polyethyleneimine (PEI)-modified mesoporous silica nanoparticles (MSNs) loaded with model antigen ovalbumin (OVA) outer disulfide bond-involved metal–phenolic networks (MPNs) as...
Epithelial ovarian cancer is prone to metastasizing at an early stage, but their mechanisms remain unclear. CRM1 important nuclear exportin and inhibitors targeting has been explored as anti-cancer strategy. In previous study, we observed that PEITC could combine with the hydrophobic pocket of CRM1. this focused on effects EOC its mechanisms. Results showed IC50 values SKOV3 HO8910 cell line were 42.14 μM 37.29 μM, respectively. inhibits migration invasion cells in vitro. Oral administration...
Abstract Human rabies remains a globally significant public health problem. Replacement of polyclonal anti-rabies immunoglobulin (RIG), passive component post-exposure prophylaxis (PEP), with monoclonal antibody (MAb), would eliminate the cost and availability constraints associated RIG. Our team has developed licensed human RAB1 (Rabishield © ), as replacement for RIG where canine is enzootic. However, highly diverse viruses North America, cocktail containing two or more MAbs targeting...
Ebola virus (EBOV) is a highly lethal member of the Filoviridae family associated with human hemorrhagic disease. Despite being sporadic disease, it caused large outbreak in 2014-2016 West Africa and another recently Democratic Republic Congo. Several vaccine candidates are currently preclinical clinical studies but none stable without cold chain storage.We used preservation by vaporization (PBV), novel processing technology to heat-stabilize FiloRab1 (inactivated rabies-based vaccine),...
Rabies is nearly 100% lethal in the absence of treatment, killing an estimated 59,000 people annually. Vaccines and biologics are highly efficacious when administered properly. Sixteen rabies-related viruses (lyssaviruses) similarly lethal, but some divergent enough to evade protection from current vaccines biologics, which based only on classical rabies virus (RABV). Here we present development characterization LyssaVax, a vaccine featuring structurally designed, functional chimeric...
Studies of HIV molecular evolution and pathogenesis have relied on the polymerase chain reaction (PCR) to provide sequence information from infected tissues. Until recently, studies been constrained by limited length fragments that can be reliably amplified. The addition a thermostable 3′-exonuclease activity altered cycling profiles has increased target sequences amplified more than 10-fold. We evaluated fidelity long PCR (LPCR). determined LPCR amplification maintains distribution found in...
As a potential method for tumor treatment, vaccine immunization induces tumor-specific cellular immune response via with antigens. The delivery of exogenous antigen proteins into the cytoplasm antigen-presenting cells is well known to induce an intensive treatment. In this work, we fluorinated redox-responsive hyperbranched poly(amidoamine) (HPAA) heptafluorobutyric anhydride prepare HPAA (HPAA-F7) use as system antitumor therapy. results show that HPAA-F7 carrier could effectively promote...
The human immunodeficiency virus type 1 protease plays a critical role in the proteolytic processing of precursor polyproteins during virion maturation. Contradictory evidence has been obtained for possible early after infection, i.e., DNA synthesis and/or integration. We have reexamined this question by using conditional mutants protease. In one set experiments, that confer temperature-sensitive phenotype were used to assess need activity infection. No significant difference from results...
Rabies is preventable through vaccination, but the need to mount annual canine vaccination campaigns presents major challenges in rabies control and prevention. The development of a vaccine that ensures lifelong immunity animal population management one dose could be extremely advantageous. A nonsurgical alternative spay/neuter high priority for welfare, irreversible infertility has not been achieved. Towards this goal, we developed virus-vectored immunocontraceptive ERA-2GnRH, which...
Abstract Yolk-shell-structured calcium phosphate microspheres have a great potential for medical applications due to their excellent physicochemical properties and biocompatibility. However, developing yolk-shell-structured with high adsorption capability remains challenge. Herein, porous microsphere (ATP-CG) of high-specific surface area [ S BET = 143 m 2 g −1 , which is approximately three times as that ATP-CL synthesized by replacing source l -lactate pentahydrate (CL)] was successfully...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTheoretical Analysis of the Elastomeric and Optical Properties Networks Semirigid Chains in Swollen StateYong Yang, A. Kloczkowski, J. E. Mark, B. Erman, I. BaharCite this: Macromolecules 1995, 28, 14, 4920–4926Publication Date (Print):July 1, 1995Publication History Published online1 May 2002Published inissue 1 July 1995https://pubs.acs.org/doi/10.1021/ma00118a020https://doi.org/10.1021/ma00118a020research-articleACS PublicationsRequest reuse...