Platinum-based doublets with a third-generation agent are the recommended option for many patients non-small cell lung cancer (NSCLC) no contraindications to platinum compounds. Unfortunately, clinical effectiveness of such chemotherapy is limited by intrinsic or acquired resistance.Circulating exosomal miRNAs were isolated and used perform HiSeq deep-sequencing analyses on serum pool samples from platinum-resistant platinum-sensitive patients, six further validated their predictive utility...

Exosomes are important mediators of intercellular communication. Previously, we characterized circulating exosomal miR-425-3p as a non-invasive prognostic marker for predicting clinical response to platinum-based chemotherapy in patients with non-small cell lung cancer (NSCLC).Circulating was validated by qRT-PCR paired serum samples from NSCLC during the course chemotherapy. Cell coculture performed examine effects on sensitivity recipient A549 cells cisplatin. Using bioinformatics, ChIP...

Colorectal cancer, a malignant neoplasm that occurs in the colorectal mucosa, is one of most common types gastrointestinal cancer. cancer has been studied extensively, but molecular mechanisms this malignancy have not characterized. This study identified and verified core genes associated with using integrated bioinformatics analysis. Three gene expression profiles (GSE15781, GSE110223, GSE110224) were downloaded from Gene Expression Omnibus (GEO) databases. A total 87 differentially...

Colorectal cancer (CRC) is one of the most common malignant tumors with a poor prognosis. At present, pathogenesis not completely clear. Therefore, finding reliable prognostic indicators for CRC important clinical significance. In this study, bioinformatics methods were used to screen immune-related lncRNAs CRC, and risk scoring model based on signatures constructed provide basis evaluation immunotherapy patients.The information RNA-seq data patients obtained from The Cancer Genome Atlas...

Lung cancer is the most common cause of cancer-related death worldwide and platinum-based drugs such as cisplatin have been used first line treatment. However, clinical effectiveness chemotherapy limited by intrinsic or acquired resistance. In this study, we found that induced autophagy attenuated sensitivity both A549 Lewis lung (LLC) cells to cisplatin. contrast, drug andrographolide (Andro) suppressed enhanced cisplatin-mediated apoptosis in these cells. Using two murine models, including...

Aims Fine roots and leaves are important belowand above-ground functional organs. It is widely recognized that leaf nitrogen (N) phosphorus (P) stoichiometry displays significant latitudinal variations, two competing theories (i.e. Temperature-Plant Physiological Hypothesis Growth Rate Hypothesis) have been proposed to explain this phenomenon. Although considerable efforts made test these theories, comparatively few data reported for the plants in China examine longitudinal variations fine...

Background and Aims Empirical studies allometric partitioning (AP) theory indicate that plant above-ground biomass (MA) scales, on average, one-to-one (isometrically) with below-ground (MR) at the level of individual trees entire forest communities. However, ability AP to predict allocation patterns understorey plants has not been established because most previous empirical tests have focused canopy tree species or very large shrubs. Methods In order test further, 1586 sub-tropical from 30...

Stroke is a neurological disease with high rates of mortality and disability. The pathogenesis stroke acute focal injury the central nervous system, leading to impaired neural function. Ischemic accounts for majority cases. At present, exact molecular mechanism ischemic remains unclear. Studies have shown that long noncoding RNAs (lncRNAs) an important regulatory role in biological processes, participating regulation transcription affecting processing splicing mRNAs. Abnormal lncRNA...

Flowering time is an important trait for ornamental plants, and flowering regulation has thus been both a focus of challenge to researchers. Lagerstroemia indica summer tree in China introduced abroad as key parent new cultivars; no previous reports have addressed the this species. In study, 28,567,778×2 reads were obtained from leaves L. indica. A total 37,325 unigenes assembled with average length 849.56 bp, 17,506 (46.90%) significantly matched known genes nr database GenBank. The...

Clematis apiifolia, belonging to the L., is a woody vine native China. It characterized as heat resistant and fast growing. To better understand potential mechanisms involved in heat-stress responses Clematis, we digital gene expression signatures of C. apiifolia under conditions. Using RNA sequencing technology, sequenced six libraries, three biological replicates control samples heat-stressed samples. In total, 61,708 unigenes were obtained, 36,447 (59.06%) which annotated. There 1941...

Chemotherapy‐induced peripheral neuropathy (CIPN) is a common side effect of anticancer treatment, which may influence its successful completion. The Huang‐Qi‐Gui‐Zhi‐Wu‐Wu decoction (HQGZWWD) has been widely used to treat CIPN in China although the pharmacological mechanisms involved have not clarified. Using network pharmacology approach, this study investigated potential pathogenesis and therapeutic exerted by HQGZWWD herbal formula CIPN. targets were identified using traditional Chinese...

Abstract Allometric biomass allocation theory predicts that leaf ( M L ) scaled isometrically with stem S and root R biomass, thus above‐ground (leaf stem) A nearly below‐ground (root) for tree seedlings across a wide diversity of taxa. Furthermore, prior studies also imply scaling constant should vary species. However, litter is known about whether such invariant isometric exponents hold intraspecific allocation, how variation in constants influences the interspecific relationship between...

Highly sensitive and accurate detection of disease biomarkers is great importance for diagnosis, staging, treatment certain diseases. Herein, we report a novel electrochemical method the quantification miRNA with DNA tetrahedrons as signal reporters. Upon initiation hairpin opening by at electrode interface, hidden click reaction group exposed bioconjugation tetrahedral nanostructure, which carries multiple species. Strand displacement polymerization reductant-mediated amplification are...

Premise of the study: Catalpa bungei (Bignoniaceae) is a deciduous tree native to China. We developed microsatellite markers for C. investigate its population genetics. Methods and Results: One hundred seventy‐seven expressed sequence tag (EST)–simple repeat (SSR) primer pairs were isolated characterized using next‐generation sequencing. Thirty these polymorphic loci in 52 individuals . The number alleles ranged from two 18 with observed expected heterozygosity values 0.05−1.00 0.18−0.95,...

Purpose: This study aimed to investigate the relationship between gut microbiota (GM) and serum metabolism using antineoplastic Fufangchangtai (FFCT) as model prescription in treatment of colorectal cancer (CRC). Methods: Tumor-bearing mice normal were administered different doses FFCT. The tumor volume tumor-bearing was observed. levels CD4 + CD8 T cells blood, spleen, determined a flow cytometer. bacterial stool samples from metabolomics FFCT-treated fecal transplantation detected 16s RNA...

In the context of high-quality and connotative development higher education institutions, this study addresses characteristics talent demand in new era, adhering to educational philosophy "people-oriented, moral education." A "Five-in-One" blended teaching model is proposed exemplified with "Cartography" course. The elaborates on optimizing design, refining content, improving schemes. Application practices demonstrate that a integrating professional courses ideological political education,...

Polychlorinated biphenyls (PCBs), a family of persistent toxic and organic environmental pollutants, were associated with multiple organ damages in humans once accumulating. However, association between PCBs exposure circulatory immune markers not clear. Data was collected from participants enrolled the National Health Nutrition Examination Survey 1999-2004. categorized by latent class analysis (LCA). Weighted quantile sum (WQS) regression used to investigate effects on including leukocyte...