A5059672534- Reproductive Biology and Fertility
- Reproductive Health and Technologies
- Sperm and Testicular Function
- Genetic Neurodegenerative Diseases
- Biochemical Analysis and Sensing Techniques
- Regulation of Appetite and Obesity
- Fungal and yeast genetics research
- Mitochondrial Function and Pathology
- Cardiomyopathy and Myosin Studies
- melanin and skin pigmentation
- Renal and related cancers
- Viral Infections and Immunology Research
- Childhood Cancer Survivors' Quality of Life
- Assisted Reproductive Technology and Twin Pregnancy
- Animal Disease Management and Epidemiology
- Neurotransmitter Receptor Influence on Behavior
- Male Reproductive Health Studies
- Receptor Mechanisms and Signaling
- Vector-Borne Animal Diseases
University Medical Center Utrecht
2014-2021
Utrecht University
2018
Radboud University Nijmegen
2006-2009
Radboud University Medical Center
2006-2009
Australian Centre for Disease Preparedness
2005
Commonwealth Scientific and Industrial Research Organisation
2005
The central melanocortin (MC) system mediates its effects on food intake via MC3 (MC3R) and MC4 receptors (MC4R). Although the role of MC4R in meal size determination, satiation, preference, motivation is well established, involvement MC3R modulation has been less explored. Here, we investigated incentive for food, which a crucial component feeding behavior. Dopaminergic neurons within ventral tegmental area (VTA) have food. We here report that MC3Rs are expressed VTA dopaminergic...
The striatum harbors two neuronal populations that enable action selection. One population represents the striatonigral pathway, expresses dopamine receptor D1 (DRD1) and promotes execution of motor programs, while other striatopallidal D2 (DRD2) suppresses voluntary activity. integrate distinct sensorimotor, cognitive emotional information streams their combined activity enables selection adaptive behaviors. Characterization these is critical to understanding role in selection, because it...
The role of the melanocortin (MC) system in feeding behavior is well established. Food intake potently suppressed by central infusion MC 3/4 receptor agonist α-melanocyte stimulating hormone (α-MSH), whereas inverse-agonist Agouti Related Peptide (AGRP) has opposite effect. receptors are widely expressed both hypothalamic and extra-hypothalamic brain regions, including nuclei involved food reward motivation, such as nucleus accumbens (NAc) ventral tegmental area. This suggests that MCs...
Background Childhood cancer patients often remain uninformed regarding their potential risk of gonadal damage. In our hospital we introduced a five step standard oncofertility care plan for all newly diagnosed female aiming to identify, inform and triage 100% counsel at high (HR) This observational retrospective study (PEARL study) evaluated the use this in first full year national cohort. Methods The steps consist 1)timely (preferably before start gonadotoxic treatment) identification new...
Studies on the myotonic dystrophy protein kinase (DMPK) gene and products have thus far mainly concentrated fate of length mutation in (CTG)n repeat at DNA level consequences expansion RNA DM1 patients disease models. Surprisingly little is known about function DMPK products.We demonstrate here that transient expression one major product human gene, hDMPK A isoform with a long tail anchor, results mitochondrial fragmentation clustering perinuclear region. Clustering occurred variety cell...
Myotonic dystrophy protein kinase (DMPK) is a Ser/Thr-type with unknown function, originally identified as the product of gene that mutated by triplet repeat expansion in patients myotonic type 1 (DM1). Alternative splicing DMPK transcripts results multiple isoforms carrying distinct C termini. Here, we demonstrate expressing individual DMPKs various cell types, including C2C12 and DMPK−/− myoblast cells, unique sequence arrangements these tails control specificity anchoring into...
Abstract Myotonic dystrophy type 1 (DM1) is a neuromuscular disorder caused by an unstable (CTG · CAG)n segment in the 3′ untranslated region of myotonic protein kinase ( DMPK ) gene. It commonly accepted that mRNA‐based toxicity main contributor to DM1 manifestations; however, not much known about significance protein. To appreciate its normal and possible pathobiological role, we analyzed patterns splice isoform expression mouse tissues. Long membrane–anchored dominated heart, diaphragm,...
In men suffering from obstructive azoospermia (OA), surgical sperm retrieval (SR) can be performed for use with intracytoplasmic injection (ICSI). Several techniques of SR exist, various results. our facility, we have developed the open epididymal spermatozoa aspiration (OESA) procedure. The aim this study was to report on rate (SRR), reproductive outcome and neonatal OESA followed by ICSI. addition, investigated possible predictors successful clinical pregnancy. A total 231 who were treated...
Objectives To evaluate treatment results of electroejaculation in patients with spinal cord injuries and the additional value repeated electroejaculation. Methods We carried out a retrospective chart analysis all injury treated at University Medical Center Utrecht, Netherlands, from January 1994 to July 2015. Data were collected on patients’ demographics medical history. evaluated sperm quality according World Health Organization standards, pregnancy delivery rates. Results A total 230...
The myotonic dystrophy protein kinase polypeptide repertoire in mice and humans consists of six different splice isoforms that vary the nature their C-terminal tails presence or absence an internal Val-Ser-Gly-Gly-Gly motif. Here, we demonstrate exist high-molecular-weight complexes controlled by homo- heteromultimerization. This multimerization is mediated coiled-coil interactions tail-proximal domain occurs independently alternatively spliced segments activity. Complex formation was...