A5100699863- Metal-Catalyzed Oxygenation Mechanisms
- Advanced Drug Delivery Systems
- Advancements in Transdermal Drug Delivery
- DNA Repair Mechanisms
- Antibiotic Resistance in Bacteria
- Metal complexes synthesis and properties
- Essential Oils and Antimicrobial Activity
- Nerve injury and regeneration
- Microbial Natural Products and Biosynthesis
- Silk-based biomaterials and applications
- Ginger and Zingiberaceae research
- Probiotics and Fermented Foods
- Biochemical Acid Research Studies
- RNA Interference and Gene Delivery
- Phytochemistry and biological activity of medicinal plants
- Bee Products Chemical Analysis
- Bacterial biofilms and quorum sensing
- Virus-based gene therapy research
- Berberine and alkaloids research
- Synthesis and Biological Evaluation
- Nanoparticle-Based Drug Delivery
- Lymphatic System and Diseases
- Postharvest Quality and Shelf Life Management
- Body Contouring and Surgery
- Bacteriophages and microbial interactions
Henan Provincial Eye Hospital
2021-2023
Henan Provincial People's Hospital
2021-2023
Zhengzhou University
2021-2023
National Institute for Radiological Protection
2021
Chinese Center For Disease Control and Prevention
2021
Nanjing Agricultural University
2021
Jinan University
2020
First Affiliated Hospital of Zhengzhou University
2019
Shanghai Ninth People's Hospital
2013-2018
Capital Medical University
2014-2018
O-linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification involving an attachment of single β-N-acetylglucosamine moiety to serine or threonine residues in nuclear and cytoplasmic proteins. Cellular O-GlcNAc levels are regulated by two enzymes: transferase (OGT) O-GlcNAcase (OGA), which add remove the modification, respectively. The can rapidly change response fluctuations extracellular environment; however, O-GlcNAcylation returns baseline level quickly after stimulus...
Abstract Background fatty acids are considered to be effective components promote wound healing and Lucilia sericata larvae applied clinically treat intractable wounds. We aimed investigat the effect of acid extracts from dried on murine cutaneuous as well angiogenesis. Results On day 7 10 after acute excision wounds creation, percent contraction group was higher than that vaseline group. 3, quality better terms granulation formation collagen organization. 3 micro vessel density vascular...
Fungal keratitis (FK) remains a severe sight-threatening disease, and case management is difficult due to ocular intrinsic barriers drug shortages. Econazole (ECZ), broad-spectrum antifungal agent, limited in applications the poor water solubility strong irritant property.We successfully prepared solid-lipid nanoparticle-based ECZ eye drops (E-SLNs) by microemulsion method, physicochemical properties of E-SLNs were investigated. Corneal permeability, ability against Fusarium spp., irritation...
Fungal keratitis is an infectious disease caused by pathogenic fungi with a high blindness rate. Econazole (ECZ) imidazole antifungal drug insoluble ability. Econazole-loaded solid lipid nanoparticles (E-SLNs) were prepared microemulsion method, then modified positive and negative charge. The mean diameter of cationic E-SLNs, nearly neutral E-SLNs anionic 18.73±0.14, 19.05±0.28, 18.54±0.10 nm respectively. Zeta potential these different charged SLNs formulations 19.13±0.89, -2.20±0.10,...
beta-Lactamase inhibitory protein (BLIP) binds and inhibits a diverse collection of class A beta-lactamases at wide range affinities. Alanine-scanning mutagenesis was previously performed to identify the amino acid sequence requirements BLIP for inhibiting TEM-1 beta-lactamase SME-1 beta-lactamase. Two hotspots binding energy, one from each domain BLIP, were identified (Zhang, Z., Palzkill, T. (2003) J. Biol. Chem. 278, 45706-45712). This study has been extended examine SHV-1 beta-lactamase,...
Indole-2-carboxylic acid copper complex (ICA-Cu) was successfully prepared and characterized through elemental analysis, IR, UV-Vis, ¹H-NMR, TG molar conductance, its molecular formula [Cu₂(C₉H₆O₂N)₄(H₂O)₂]·2H₂O. The binding ability of ICA-Cu to calf thymus DNA (CT-DNA) examined by fluorescence spectrometry the viscosity method. results indicated that, upon addition increasing amounts CT-DNA, excitation emission intensity decreased obviously spectra shifted towards a long wavelength. could...
Ribonucleotide reductase maintains cellular deoxyribonucleotide pools and is thus tightly regulated during the cell cycle to ensure high fidelity in DNA replication. The Sml1 protein inhibits ribonucleotide activity by binding R1 subunit. At completion of each turnover cycle, active site becomes oxidized subsequently regenerated a cysteine pair (CX2C) at its C-terminal domain (R1-CTD). Here we show that R1-CTD acts trans reduce neighboring monomer. Both interact with N-terminal (R1-NTD),...
The preclinical studies using animal models play a very important role in the evaluation of facial nerve regeneration. Good need to recapitulate distance and time for axons regenerate humans. Compared with most used rodent animals, structure mini-pigs shares more similarities humans microanatomy. To evaluate feasibility repairing defects by collagen scaffolds combined ciliary neurotrophic factor (CNTF), 10-mm-long gaps were made buccal branch mini-pigs' nerve. Three months after surgery,...
The class I ribonucleotide reductases (RNRs) are composed of two homodimeric subunits: R1 and R2. R2 houses a diferric-tyrosyl radical (Y*) cofactor. Saccharomyces cerevisiae has R2s: Y2 (beta2) Y4 (beta'2). is an unusual because three residues required for iron binding have been mutated. While the heterodimer (betabeta') thought to be active form, several rnr4delta strains viable. To resolve this paradox, N-terminally epitope-tagged beta beta' were expressed in E. coli or integrated into...
Tumor is a prevalent great threat to public health worldwide and multidrug resistance (MDR) of tumor leading cause chemotherapy failure. Nanomedicine has shown prospects in overcoming the problem. Doxorubicin (DOX), broad-spectrum anticancer drug, showed limited efficacy due MDR. Herein, doxorubicin containing pectin nanocell (DOX-PEC-NC) core-shell structure, nanoparticle encapsulated with liposome-like membrane was developed. The DOX-PEC-NC, spheroid shape sized around 150 nm, exerted...
Adipocytes attached to the extracellular matrix (ECM) mainly consist of collagen in adipose tissues, while degradation ECM by collagenase induces apoptosis adipocytes, leading a decrease local subcutaneous adipose. To achieve this goal, we are developing mutant H (ColH) remove fat such as submental (SMF). Three vectors were constructed for expressing rColH(FM, melting, with 6xHis tag), rColH(WT, wild-type, and rColH(E451D, E451D mutant, without tag) Escherichia coli. rColH(FM) & rColH(WT)...
Objective To prepare arginine-glycine-aspartic acid (RGD)-and ribonuclease A (RNase A)-conjugated CdTe quantum dot (QD) nanoprobes,and to observe their capability target human A375 malignant melanoma cells.Methods RNase A-modified dots (CdTe RQDs) were obtained by using a microwave-based heating method,and then chemically conjugated the RGD peptide RGD-CdTe RQD nanoprobes,which physically and characterized transmission electron microscopy,powder crystal diffraction,fluorescence...
The objective of this research was to explore the dose-effect relationships dicentric plus ring (dic + r), micronucleus (MN) and nucleoplasmic bridges (NPB) induced by carbon ions in human lymphocytes.