A5103126082- Nerve injury and regeneration
- Orthopedic Surgery and Rehabilitation
- Wound Healing and Treatments
- Tendon Structure and Treatment
- Spinal Cord Injury Research
- Lower Extremity Biomechanics and Pathologies
- Forensic Entomology and Diptera Studies
- Insect Utilization and Effects
- Cancer-related molecular mechanisms research
- Foot and Ankle Surgery
- Bee Products Chemical Analysis
- Trauma Management and Diagnosis
- Neurogenesis and neuroplasticity mechanisms
- Mesenchymal stem cell research
- Adenosine and Purinergic Signaling
- Diabetic Foot Ulcer Assessment and Management
- Bone fractures and treatments
- Drug Transport and Resistance Mechanisms
- Orthopaedic implants and arthroplasty
- Shoulder and Clavicle Injuries
- Spinal Dysraphism and Malformations
- Computational Geometry and Mesh Generation
- Pregnancy-related medical research
- Sarcoma Diagnosis and Treatment
- Phytoestrogen effects and research
Dalian Medical University
2014-2024
First Affiliated Hospital of Dalian Medical University
2013-2024
In-Q-Tel
2014
Peking University
2010-2012
Abstract Background fatty acids are considered to be effective components promote wound healing and Lucilia sericata larvae applied clinically treat intractable wounds. We aimed investigat the effect of acid extracts from dried on murine cutaneuous as well angiogenesis. Results On day 7 10 after acute excision wounds creation, percent contraction group was higher than that vaseline group. 3, quality better terms granulation formation collagen organization. 3 micro vessel density vascular...
Human osteosarcoma usually presented a high tendency to metastatic spread and caused poor outcomes, however, the underlying mechanism was still largely unknown. In present study, using series of in vitro experiments an animal model, we investigated roles HOX antisense intergenic RNA (HOTAIR) during proliferation invasion osteosarcoma. According with our results, HOTAIR commonly overexpressed osteosarcoma, which significantly correlated advanced tumor stage, highly histological grade...
Maggots (larvae of Lucilia sericata) have shown therapeutic effects on refractory wounds infected with bacteria, yet the bacterial killing mechanisms are unclear. Herein, we report isolation and purification an antibacterial protein from maggots (MAMP). MAMP demonstrated inhibitory activity against both standard strains clinically isolated antibiotic-resistant Staphylococcus aureus in vitro. The topical use effectively decreased viability S. promoted wound healing mouse skin infection model....
Objectives: DFU is a serious chronic disease with high disability and fatality rates, yet there no completely effective therapy. While ferroptosis integrated to inflammation infection, its involvement in still unclear. The study aimed identify ferroptosis-related genes DFU, providing potential therapeutic targets. Methods: In the GEO database, two microarray datasets (GSE147890 GSE80178) were collected. WGCNA was conducted modular most involved DFU. Subsequently, enrichment analysis PPI...
Currently, Posterior Short Segment Pedicle Screw Fixation is a popular procedure for treating unstable thoracolumbar/lumbar burst fracture. But progressive kyphosis and high rate of hardware failure because lack the anterior column support remains concern. The efficacy different methods debatable each technique has its advantages disadvantages. A consecutive series 20 patients with isolated fractures were treated by posterior short segment pedicle screw fixation transforaminal interbody...
Abstract Maggot extracts promote wound healing, but their bioactive part(s) and molecular effects on the regenerating tissues/cells remain largely unclear. These issues are addressed here by treating rat skin wounds, human keratinocyte line/ HaCat fibroblasts with maggot secretion/excretion, of maggots without secretion/excretion. The closure rates, cell proliferation activities, statuses healing‐related signaling pathways ( STAT 3, Notch 1, Wnt 2, NF ‐κ B , TGF ‐beta/Smad3) downstream gene...
Clinically there are different fixation methods used for of the posterior malleolar fractures (PMF), but best treatment modality is still not clear. Few studies have concentrated on this issue, least all using a biomechanical comparison. The purpose study was to carry out computational comparative biomechanics three commonly constructs PMF by finite element analysis (FEA). Computed tomography (CT) images were reconstruct dimensional (3D) model tibia. Computer aided design (CAD) software 3D...
Recent studies regarding regenerative medicine have focused on bone marrow mesenchymal stem cells (BMSCs), which the potential to undergo neural differentiation, and may be transfected with specific genes. BMSCs can differentiate into neuron‑like in certain neurotropic circumstances vitro. Basic fibroblast growth factor (bFGF) nerve (NGF) are often used induce differentiation However, previous their combined actions insufficient. The present study is first, best of our knowledge, thoroughly...
Following spinal cord injury in rats, FK506 is able to protect local nerve tissue, promote neural regeneration, reduce neuronal apoptosis and accelerate the recovery of functions. Nerve growth factor (NGF) important regulation central peripheral cell differentiation Previous studies have shown that NGF exhibit a synergistic effect treatment injury; however, it remains unclear whether present injury. In this study, we combined for rats. The NF200 protein expression rats with was determined...
The present study aimed to investigate the reversal effect of resveratrol on phenomenon multidrug resistance in U2OS/adriamycin (ADR) cells and clarify molecular mechanisms. To examine cell survival half‑inhibitory concentration (IC50) ADR U2OS U2OS/ADR cells, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used. accumulation investigated by flow cytometry. Reverse transcription‑quantitative polymerase chain reaction western blot analysis were used detect expression...
Wound measurement is an objective and direct way to trace the course of wound healing evaluate therapeutic efficacy. Nevertheless, accuracy efficiency current methods need be improved. Taking advantages reliability transparency tracing computer-aided digital imaging, a transparency-based imaging approach established, by which data from 340 were collected 6 experimental groups (8 rats/group) at 8 time points (Day 1, 3, 5, 7, 10, 12, 14 16) orderly archived onto model sheet. This sheet was...
Abstract Different fixation modalities are available for of posterior malleolar fractures (PMFs), but the best method is still unclear. The purpose this study was to carry out a comparative biomechanical analysis three commonly used constructs PMFs using experimental and finite element (FEA). 15 human cadaveric ankle specimens were randomly divided into groups. Specimens in group-A fixed with two anteroposterior (AP) lag screws, group-B posteroanterior (PA) group-C, plate used. Each model...
Numerous researches demonstrated the possibility of derivation Schwann-like (SC-like) cells in vitro from bone marrow stromal (BMSCs). However, concentration induce factors were different those studies, especially for critical forskolin (FSK) and β-heregulin (HRG). Here, we used a new useful method to build an integrated microfluidic chip rapid analyses optimal combination between FSK HRG. The device was mainly composed upstream gradient generator (CGG) downstream cell culture module. Rat...
Abstract Background Neurofibromas may occur as part of neurofibromatosis or a solitary tumor. Solitary subungual neurofibroma appears to be rare condition. Up until now, less than 10 case reports have been documented. is difficult diagnose, particularly it often small and without obvious symptoms. Awareness the diagnosis emphasized prevent unnecessary delay in treatment. So, understanding differentiation has great value. Methods A 32‐year‐old woman presented with two‐year history painless...
Abstract A highly selective and sensitive liquid chromatography–tandem mass spectrometry has been developed validated for simultaneous determination of three isoflavones – ononin, formononetin biochanin in rat plasma using lysionotin as internal standard (IS). The samples were pretreated extracted by liquid–liquid extraction. Chromatographic separation was accomplished on a C 18 column with the temperature 30 °C mobile phase methanol–0.1% formic acid (75:25, v/v). detection multiple‐reaction...
AIM:To investigate the antibacterial effects of a crude extract maggots against Escherichia coli (E. ) and underlying mechanisms, to separate purify assess active ingredients in extract.
Mouse zygotes are widely used in developmental biology and transgenic animal research. Two-photon laser scanning microscopy is particularly useful four-dimensional observation of big thick biological samples, such as mouse embryo. The early embryo development from zygote to 8-cell stage compaction was observed real-time by stages using two-photon this paper. During our experiment, several parameters were optimized different stages. initial cleavages embryo, the 2-cell, 2-cell 4-cell, 4-cell...
To investigate the impact of subchondral bone cysts (SBCs) in stress-induced osseous and articular variations cystic non-cystic knee models using finite element analysis.3D joint were reconstructed from computed tomography (CT) magnetic resonance imaging (MRI). Duplicate 3D also created with a sphere mimicking SBCs medial tibia. Models divided into three groups. In group A, model was used, whereas groups B C, 4 12 mm size simulated, respectively. Cyst further sub-groups. Each sub-group 1...