A5014963443- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Nerve injury and regeneration
- Orthopedic Surgery and Rehabilitation
- Plant Pathogens and Fungal Diseases
- Yeasts and Rust Fungi Studies
- Berry genetics and cultivation research
- Neurofibromatosis and Schwannoma Cases
- Cancer-related gene regulation
- Chromosomal and Genetic Variations
- HER2/EGFR in Cancer Research
- Elbow and Forearm Trauma Treatment
- Genomics and Phylogenetic Studies
- Animal Genetics and Reproduction
- Plant Reproductive Biology
- Neurogenesis and neuroplasticity mechanisms
- CRISPR and Genetic Engineering
- Breast Cancer Treatment Studies
- Cancer-related Molecular Pathways
- Shoulder Injury and Treatment
- Genetic Syndromes and Imprinting
- Breast Lesions and Carcinomas
- GABA and Rice Research
- Sarcoma Diagnosis and Treatment
- Soft tissue tumors and treatment
Takeda (Japan)
2024
Wuhan General Hospital of Guangzhou
2024
Third Hospital of Nanchang
2012-2023
The University of Tokyo
2014-2023
Dalian Medical University
2008-2022
First Affiliated Hospital of Dalian Medical University
2009-2022
Wuhan University of Science and Technology
2020-2021
Wayne State University
2020-2021
Nanjing Agricultural University
2016-2020
Second Affiliated Hospital of Xi'an Jiaotong University
2016-2020
The medaka fish (Oryzias latipes) is a popular pet in Japan and more recently laboratory model organism for developmental genetics evolutionary biology. Now the medaka's genome has been sequenced analysed by large Japanese consortium. Cichlids stickleback, which are emerging systems understanding genetic basis of vertebrate speciation, evolutionarily closer to than zebrafish, so sequence will yield valuable insights into 400 million years evolution. long organism; it now its Teleosts...
To investigate the expression and role of long non-coding RNA (lncRNA) small nucleolar host gene 12 (SNHG12) in papillary thyroid carcinoma (PTC).The relative levels lncRNA SNHG12 (hereinafter referred to as SNHG12) 42 pairs PTC tissues para-carcinoma were detected via quantitative reverse transcription polymerase chain reaction (qRT-PCR). specific interference sequences designed synthesized. The level transfection efficiency cells qRT-PCR. After expression, change cell proliferation...
Abstract Alveolar soft part sarcoma (ASPS) is a malignancy affecting adolescents and young adults. ASPS characterized by highly integrated vascular network, its high metastatic potential indicates the importance of ASPS’s prominent angiogenic activity. Here, we find that expression ASPSCR1::TFE3 , fusion transcription factor causatively associated with ASPS, dispensable for in vitro tumor maintenance; however, it required vivo development via angiogenesis. frequently super-enhancers (SEs)...
Prostate cancer is the second leading cause of cancer-related deaths among men worldwide. Early diagnosis increases survival rates in patients but rate has remained relatively poor over past years. Increasing evidence shows that altered metabolism a critical hallmark prostate cancer. There strong need to explore molecular mechanisms underlying for therapy. Whether aberrant expression microRNA (miRNA) contributes not fully known. In this study, we found microRNA-132 (miR-132) reduced and thus...
Triple-negative breast cancer (TNBC) cells do not express estrogen receptors, progesterone or human epidermal growth factor receptor 2. Currently, apart from poly ADP-ribose polymerase inhibitors, there are few effective therapeutic options for this type of cancer. Here, we present comprehensive characterization the genetic alterations in TNBC performed by high coverage whole genome sequencing together with transcriptome and exome sequencing. Silencing BRCA1 gene impaired homologous...
Novel massively parallel sequencing technologies provide highly detailed structures of transcriptomes and genomes by yielding deep coverage short reads, but their utility is limited inadequate quality short-read lengths. Sequencing-error trimming in reads therefore a vital process that could improve the rate successful reference mapping polymorphism detection. Toward this aim, we herein report frequency-based, de novo clustering method organizes erroneous sequences originating single...
5-methyl-cytosines at CpG sites frequently mutate into thymines, accounting for a large proportion of spontaneous point mutations. The repair system would leave substantial numbers errors in neighboring regions if the synthesis erased gaps around deaminated is error-prone. Indeed, we identified an unexpected genome-wide role methylation state as major determinant proximal natural genetic variation. Specifically, 507 Mbp (∼18%) human genome was within 10 bp site; these regions, single...
Memory CD4(+) T cells are central regulators of both humoral and cellular immune responses. cell differentiation results in specific changes chromatin structure DNA methylation cytokine genes. Although the status a limited number gene loci has been examined, genome-wide memory remains unexplored. To further elucidate molecular signature cells, we conducted methylome transcriptome analyses generated using from TCR-transgenic mice. The resulting profile revealed 1144 differentially methylated...
DNA methylation is a fundamental epigenetic modification in vertebrate genomes and small fraction of genomic regions hypomethylated. Previous studies have implicated hypomethylated gene regulation, but their functions development remain elusive. To address this issue, we generated epigenomic profiles that include base-resolution methylomes histone maps from both pluripotent cells mature organs medaka fish compared the with those human ES cells. We found subset domains harbor H3K27me3...
Organic acid is an important indicator of fruit quality, and malate the predominant organic in apple fruit. However, regulation metabolism postharvest rarely reported. Here, we found that, compared with a control treatment, 10 mM γ-aminobutyric (GABA) treatment remarkably delayed loss tiftratable acidity increased succinate oxalate contents "Cripps Pink" stored polyethylene bags at room temperature. The higher levels GABA-treated were accompanied by activities cytosolic nicotinamide adenine...
Saccharin sodium consumption is considered safe and beneficial, owing to its very intense sweetness without any associated calories, but supporting scientific data remain sparse controversial. Herein, we demonstrate that dose-response relationships existed with regard administration of saccharin or sucrose mice for 35 days, this association involved testis-expressed sweet-tasting molecules (taste receptor type 1 subunit 3 [T1R3]; G protein alpha-gustducin [Galpha]). Mouse body weights testis...
Intraflagellar transport (IFT) is an evolutionarily conserved mechanism that indispensable for the formation and maintenance of cilia flagella; however, implications functions IFT81 remain unknown. In this study, we disrupted expression in male germ cells starting from spermatocyte stage. As a result, homozygous mutant males were completely infertile displayed abnormal sperm parameters. addition to oligozoospermia, spermatozoa presented dysmorphic nonfunctional flagella. Histological...
Massively parallel, tag-based sequencing systems, such as the SOLiD system, hold promise of revolutionizing study whole genome gene expression due to number data points that can be generated in a simple and cost-effective manner. We describe development 5'-end transcriptome workflow for system demonstrate advantages sensitivity dynamic range offered by this application over traditional approaches expression. analysis was used within colon cancer cell line, HT-29, treated with DNA...
Determining the methylation state of regions with high copy numbers is challenging for second-generation sequencing, because read length insufficient to map reads uniquely, especially when repetitive are long and nearly identical each other. Single-molecule real-time (SMRT) sequencing a promising method observing such regions, it not vulnerable GC bias, produces lengths, its kinetic information sensitive DNA modifications.
Both lyophilization and electrospinning are commonly used to make chitosan scaffolds. However, it remains unknown which method is better for cell growth. In this study, we established the following groups: (1) group-chitosan scaffolds were prepared by seeded with Schwann cells from Sprague-Dawley rats aged 3-5 days; (2) cells; (3) control group-Schwann cultured on culture dishes. The growth of was evaluated immunofluorescence scanning electron microscopy. Western blot assay performed explore...