A5040014580- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Pneumonia and Respiratory Infections
- Bacterial Infections and Vaccines
- Neonatal and Maternal Infections
- Integrated Circuits and Semiconductor Failure Analysis
- Semiconductor materials and devices
- Semiconductor Quantum Structures and Devices
- Mesenchymal stem cell research
- Endodontics and Root Canal Treatments
- Quantum and electron transport phenomena
- GaN-based semiconductor devices and materials
- Silicon and Solar Cell Technologies
- Genomics and Phylogenetic Studies
- Semiconductor materials and interfaces
- Infective Endocarditis Diagnosis and Management
- Copper Interconnects and Reliability
- Spectroscopy and Laser Applications
- Muon and positron interactions and applications
- Periodontal Regeneration and Treatments
- Wound Healing and Treatments
- Tissue Engineering and Regenerative Medicine
- Advanced Materials Characterization Techniques
- Silicon Nanostructures and Photoluminescence
- Dental materials and restorations
Osaka University
2016-2025
University of California, San Diego
2020-2024
University of Osijek
2018
Nagoya University
2014-2018
National Center for Geriatrics and Gerontology
2014-2016
Renesas Electronics (Japan)
2008-2013
Renesas Electronics (United States)
2004-2011
Semiconductor Energy Laboratory (Japan)
2009-2010
Meijo University
2006-2007
Osaka City University
2002-2006
Dental pulp stem cell (DPSC) subsets mobilized by granulocyte-colony-stimulating factor (G-CSF) are safe and efficacious for complete regeneration. The supply of autologous tissue, however, is very limited in the aged. Therefore, alternative sources mesenchymal stem/progenitor cells (MSCs) needed therapy. In this study, DPSCs, bone marrow (BM), adipose tissue (AD)-derived same individual dog were isolated using G-CSF-induced mobilization (MDPSCs, MBMSCs, MADSCs). positive rates CXCR4 G-CSFR...
The secretome obtained from stem cell cultures contains an array of neurotrophic factors and cytokines that might have the potential to treat neurodegenerative conditions. Alzheimer's disease (AD) is one most common human late onset sporadic disorders. Here, we investigated therapeutic derived dental pulp cells (DPSCs) reduce cytotoxicity apoptosis caused by amyloid beta (Aβ) peptide. We determined whether DPSCs can secrete Aβ-degrading enzyme, neprilysin (NEP), evaluated effects NEP...
Streptococcus pyogenes causes mild human infections as well life-threatening invasive diseases. Since the mutations known to enhance virulence date account for only half of severe infections, additional mechanisms/mutations need be identified. Here, we conducted a genome-wide association study emm 89 S. strains comprehensively identify pathology-related bacterial genetic factors (SNPs, indels, genes, or k-mers). Japanese ( n =311) and global =666) cohort studies isolated from non-invasive...
Frailty is gaining attention worldwide with the aging of society. Despite potential lethality and multiple signs symptoms in affected individuals, preclinical detection early manifestations leading to frailty syndrome have not been established. We speculated that composition oral microbiota associated general frailty, as well a relationship between gut health condition. In present study, we investigated salivary samples from healthy frail elderly individuals using 16S rRNA sequencing...
The arginine deiminase (ADI) pathway has been found in many kinds of bacteria and functions to supplement energy production provide protection against acid stress. Streptococcus pyogenes ADI is upregulated upon exposure various environmental stresses, including glucose starvation. However, there are several unclear points about the advantages organism for upregulating catabolism. We show that contributes bacterial viability pathogenesis under low-glucose conditions. S. changes global gene...
The rise in hospital outbreaks of multidrug-resistant Acinetobacter baumannii infections underscores the urgent need for alternatives to traditional broad-spectrum antibiotic therapies. success A. as a significant nosocomial pathogen is largely attributed its ability resist antibiotics and survive environmental stressors. However, there limited literature available on global, complex regulatory circuitry that shapes these phenotypes. Computational tools can assist elucidation ’s...
Streptococcus pyogenes causes mild human infections as well life-threatening invasive diseases. Since the mutations known to enhance virulence date account for only half of severe infections, additional mechanisms/mutations need be identified. Here, we conducted a genome-wide association study emm 89 S. strains comprehensively identify pathology-related bacterial genetic factors (SNPs, indels, genes, or k-mers). Japanese ( n =311) and global =666) cohort studies isolated from non-invasive...
<title>Abstract</title> Serum amyloid A1 (SAA1), an acute-phase protein, exhibits a decreased level in bronchoalveolar lavage fluid (BALF) following nasal <italic>Streptococcus pneumoniae</italic> infections. However, the function of SAA1 relation to <italic>S. remains be unclear. In this study, we investigate whether utilized for its survival host or not. We explored by initially comparing serum with high levels low levels. then validated our findings using recombinant SAA1. research,...
can cause a wide variety of acute infections throughout the body its human host. An underlying transcriptional regulatory network (TRN) is responsible for altering physiological state bacterium to adapt each unique host environment. Consequently, an in-depth understanding comprehensive dynamics
Streptococcus pyogenes is a major cause of necrotizing fasciitis, life-threatening subcutaneous soft-tissue infection. At the host infection site, local environment and interactions between bacteria have effects on bacterial gene expression profiles, while pattern S. related to this disease remains unknown. In study, we used mouse model fasciitis performed RNA-sequencing (RNA-seq) analysis M1T1 strain 5448 by isolating total RNA from infected hind limbs obtained at 24, 48, 96 h...
Abstract Epitaxial lateral overgrowth (ELO) of low‐dislocation‐density AlN layers on trench‐patterned sapphire substrates is demonstrated for the first time. Sapphire (0001) with trench patterns formed along two different directions, 〈10 $ 10 \bar 0〉 and 〈11 11 20 0〉, were used. We can obtain fully coalesced only substrate having trenches. The dislocation density ELO‐AlN as low 6.7 × 8 cm –2 . (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Abstract Group B Streptococcus (GBS) is a leading cause of bacterial sepsis and meningitis in newborns. GBS possesses protein with homology to the pneumococcal virulence factor, NanA, which has neuraminidase (sialidase) activity promotes blood-brain barrier penetration. However, phylogenetic sequence enzymatic analyses indicate NanA ortholog lost sialidase function – for this distinction we designate gene encoded nonA /NonA. Here analyze NonA pathogenesis, through heterologous expression...
Evolutionarily conserved virulence factors can be candidate therapeutic targets or vaccine antigens. Here, we investigated the evolutionary selective pressures on 16 pneumococcal choline-binding cell-surface proteins since Streptococcus pneumoniae is one of pathogens posing greatest threats to human health. Phylogenetic and molecular analyses revealed that cbpJ had highest codon rates total numbers codons under considerable negative selection among those examined. Our in vitro vivo assays...
Dental pulp stem cells (DPSCs) are reported as sources of mesenchymal (MSCs). MSCs used cell therapy options for various diseases. The present study examined the healing effects DPSC injection on damaged bladder tissue in a chemically induced cystitis rat model. Cystitis was by hydrochloride into female F344/NSlc rats. On following day, DPSCs suspended phosphate-buffered saline (PBS) were injected and maintained 1 h (DPSC group), while PBS alone standard comparison (PBS group). After 2 days...
Streptococcus pneumoniae is a Gram-positive bacterium belonging to the oral streptococcus species, mitis group. This pathogen leading cause of community-acquired pneumonia, which often evades host immunity and causes systemic diseases, such as sepsis meningitis. Previously, we reported that PfbA β-helical cell surface protein contributing pneumococcal adhesion invasion human epithelial cells in addition its survival blood. In present study, investigated role pathogenesis. Phylogenetic...
Vital pulp therapy is an important endodontic treatment. Strategies using growth factors and biological molecules are effective in developing capping materials based on wound healing by the dentin-pulp complex. Our group developed biodegradable viscoelastic polymer for tissue-engineered medical devices. The contents help overcome poor fracture toughness of hydroxyapatite (HAp)-facilitated osteogenic differentiation cells. However, composition this novel remained unclear. This study evaluated...
Streptococcus pneumoniae is a leading cause of bacterial meningitis. Here, we investigated whether pneumococcal paralogous zinc metalloproteases contribute to meningitis onset. Findings codon-based phylogenetic analyses indicated 3 major clusters in the Zmp family; ZmpA, ZmpC, and ZmpB, with ZmpD as subgroup. In vitro invasion assays human brain microvascular endothelial cells (hBMECs) showed that deletion zmpC gene S. strain TIGR4 significantly increased into hBMECs, whereas either zmpA or...
The induction of tissue mineralization and the mechanism by which surface pre-reacted glass-ionomer (S-PRG) cement influences pulpal healing remain unclear. We evaluated S-PRG cement-induced tertiary dentin formation in vivo, its effect on pulp cell process vitro. Induced was with micro-computed tomography (μCT) scanning electron microscopy (SEM). distribution elements from confirmed micro-X-ray fluorescence (μXRF). effects cytotoxicity, proliferation, mineralized nodules, gene expression...
Surface-associated, coiled-coil M proteins of Streptococcus pyogenes (Strep A) disable human immunity through interaction with select proteins. However, coiled coils lack features typical protein–protein sites, and it is therefore challenging to understand how achieve specific binding, for example, the antimicrobial peptide LL-37, leading its neutralization. The crystal structure a complex LL-37 M87 protein, an antigenic protein variant from strain that emerging threat, revealed novel mode....
is responsible for a range of diseases in humans contributing significantly to morbidity and mortality. Among more than 200 serotypes
Streptococcus pneumoniae is a major pathogen that causes pneumonia, sepsis, and meningitis. The candidate combox site 4 (ccs4) gene has been reported to be pneumococcal competence-induced gene. Such genes are involved in development of S. competence virulence, though the functions ccs4 remain unknown. In present study, role Ccs4 pathogenesis meningitis was examined. We initially constructed deletion mutant complement strains, then examined their association with invasion into human brain...
Streptococcus pneumoniae is a major cause of invasive diseases such as pneumonia, meningitis, and sepsis, with high associated mortality. Our previous molecular evolutionary analysis revealed that the S. gene bgaA , encoding enzyme β-galactosidase (BgaA), had proportion codons under negative selection among examined pneumococcal genes deletion significantly reduced host mortality in mouse intravenous infection assay. BgaA multifunctional protein plays role cleaving terminal galactose N...