A5102865163- Respiratory viral infections research
- Influenza Virus Research Studies
- Virology and Viral Diseases
- interferon and immune responses
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Bacteriophages and microbial interactions
- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- Immune responses and vaccinations
- Animal Disease Management and Epidemiology
- Viral Infections and Outbreaks Research
- RNA and protein synthesis mechanisms
- Zoonotic diseases and public health
- Microbial infections and disease research
- Molecular Biology Techniques and Applications
- Toxoplasma gondii Research Studies
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Vibrio bacteria research studies
- Hepatitis Viruses Studies and Epidemiology
Zhejiang Center for Disease Control and Prevention
2011-2022
Academy of Military Medical Sciences
2016
Centers for Disease Control and Prevention
2009-2014
Beijing Fengtai Disease Prevention and Control Center
2014
Chinese Center For Disease Control and Prevention
2013
Hangzhou Center for Disease Control and Prevention
2011
Although Ma Huang Tang (MHT) has long been considered as a classical formula for respiratory infections like influenza, bronchitis and asthma, its chemical ingredients really exert the antiviral efficacy are still obscure. In this study we aimed to screen components investigate potential mechanisms. The MDCK cellular research results showed that, among nine predominant of MHT, L-methylephedrin (LMEP), L-ephedrine (LEP), D-pseudoephedrine (DPEP) significantly inhibited proliferation influenza...
Influenza represents a serious public health concern. The emergence of resistance to anti-influenza drugs underlines the need develop new drugs. This study aimed evaluate viral activity and possible mechanisms 12 phenanthrenes from medicinal plant Bletilla striata (Orchidaceae family).Twelve were isolated identified B. striata. virus A/Sydney/5/97 (H3N2) propagated in embryonated chicken eggs was used. Phenanthrenes mixed with incubated at 37 °C for 1 h then inoculated into 9-day-old via...
ABSTRACT As part of a national rotavirus surveillance activity, we collected fecal specimens from 3,177 children with acute diarrhea in 10 regions China between April 1998 and 2000 screened them for rotavirus. Rotavirus was detected 41% ( n = 1,305) specimens, these, G1 the predominant serotype (72.6%), followed by G3 (14.2%), G2 (12.1%), G4 (2.5%), G9 (0.9%), G untypeable (0.7%). Among 327 G-typed strains tested P genotype, 14 different P-G combinations were identified, globally common...
The diversity of pathogens associated with acute respiratory infection (ARI) makes diagnosis challenging. Traditional pathogen screening tests have a limited detection range and provide little additional information. We used total RNA sequencing ("meta-transcriptomics") to reveal the full spectrum microbes paediatric ARI. Throat swabs were collected from 48 ARI patients 7 healthy controls. Samples subjected meta-transcriptomics determine presence abundance viral, bacterial, eukaryotic...
West Nile virus (WNV) causes a severe zoonosis, which can lead to large number of casualties and considerable economic losses. A rapid accurate identification methodfor WNV for use in field laboratories is urgently needed. Here, method utilizing reverse transcription loop-mediated isothermal amplification combined with vertical flow visualization strip (RT-LAMP-VF) was developed detect the envelope (E) gene WNV. The RT-LAMP-VF assay could 102 copies/μl ofan RNA standard using 40 min reaction...
BackgroundHuman infection with a novel avian-origin influenza A (H7N9) virus occurred continuously in China during the first half of 2013, high infectivity and pathogenicity to humans. In this study, we investigated origin internal genes H7N9 analyzed relationship between virus. Methodology Principal findingsWe tested environmental specimens using real-time RT-PCR assays isolated five H9N2 viruses from that were positive for both H7 H9. Results recombination phylogeny analysis, performed...
Along with the improving vaccine coverage, suspected vaccine-associated measles has been reported in Zhejiang Province, China. In order to maintain accuracy of surveillance system, it is critical discriminate between and wild-type virus.Eight cases were Province during 2011 2014. Sera collected within 4 days throat swabs 6 after rash onset tested immunoglobulin M virus (MeV) RNA confirm MeV infection. further identify cases, positive using an allelic discrimination real-time reverse...
Dengue fever (DF) is often asymptomatic in endemic areas. Asymptomatic infection during a DF outbreak China, where not endemic, has been reported until now. In this study total of 365 subjects from 6 villages were recruited October 4–7, 2009. Overall, 102 (27.95%) positive for dengue virus (DENV) IgM, and 14 (3.84%) DENV IgG IgM. different age groups, seropositive rates varied 12.50% to 50.00% 0% 11.76% IgG. Seroprevalence IgM was significantly higher than that differed among villages....
Context Yinhuapinggan granule (YHPG) is frequently used for treating fever, cough, and viral pneumonia in traditional Chinese medicine.Objective This study investigated the antiviral effects of YHPG H1N1 influenza virus (IFV)-infected mice its possible mechanism.Materials methods ICR were intranasally infected with 10 LD50 dose IFV then oral administration (6, 12, 18 g/kg) or oseltamivir (positive control) once a day 2 4 consecutive days, six each group. The lung, spleen thymus indexes...
Rubella virus (RV) isolation is recommended by the WHO Measles and Labnet for studying etiology epidemiology of rubella. However, absence cytopathologic effects (CPE) in many cell lines used commonly makes it difficult to confirm RV growth. In this study, two assays amplifying cDNA were developed validated order genotype isolates after culture. A SYBR Green I-based real-time PCR (Rtime-SGE317) was established initial rapid detection Vero cells a nested (PCR-E860) further 739 nt window E1...
Biofilm formation is a well-known pathogenic mechanism in infections caused by Acinetobacter baumannii. Recently, biofilm synthesis-associated gene has been found A. baumannii ATCC19606. Bioinformatic analysis showed 2 transmembrane structures and an hmsS superfamily domain, which was related to formation. What more, high homology sequences of the bfs were only present spp., similarities nucleotide from strains ATCC17978, ACICU, S1, AB307-0294, AB0057 compared with reported sequence (GenBank...
Objective To determine a dengue fever outbreak in Yiwu city,Zhejiang Province 2009 and to trace the origin of pathogen. Methods The virus IgM, IgG antibodies viral nucleic acid were detected was isolated using 40 serum samples from suspected patients. RNA strains extracted E gene amplified by RT-PCR. amplicons sequenced phylogenetic homological analyses also constructed. Results Among patients, 17 positive for IgM (42. 5%); 4 (10. 0%); 34 (85.0%), 28 type 3 (D3) (70. 0%). complete coding...
Objective To Characterize the genetic diversity of hemagglutinin(HA)and neuraminidase(NA)of influenza B viruses isolated in Zhejiang province during 1999-2010.Methods Respiratory specimens were collected from patients with flu-like syndrome outbreaks or hospitals which carrying out surveillance project province.Samples detected by real-time RT-PCR and for virus.HA1 NA genes virus isolates amplified sequenced.Phylogenetic comparison analysis performed using bioinformation software.Results A...