A5100762674- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Virology and Viral Diseases
- Viral Infections and Vectors
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Viral Infections and Outbreaks Research
- Mosquito-borne diseases and control
- Virus-based gene therapy research
- interferon and immune responses
- Viral gastroenteritis research and epidemiology
- Intraocular Surgery and Lenses
- Rabies epidemiology and control
- SARS-CoV-2 detection and testing
- Immune Response and Inflammation
- Long-Term Effects of COVID-19
- Animal Disease Management and Epidemiology
- Ophthalmology and Visual Impairment Studies
- Immune Cell Function and Interaction
- Plasma Applications and Diagnostics
- Retinal and Macular Surgery
- Vector-Borne Animal Diseases
- Monoclonal and Polyclonal Antibodies Research
- Ocular Diseases and Behçet’s Syndrome
Chinese Academy of Agricultural Sciences
2019-2025
Tianjin Medical University
2008-2024
Jilin Academy of Agricultural Sciences
2023-2024
Guangxi Veterinary Research Institute
2024
Yangzhou University
2020-2024
Tianjin Medical University Eye Hospital
2008-2024
Sheng Jing Hospital
2023
Zhejiang University
2023
Institute of Special Animal and Plant Sciences
2021-2023
Academy of Military Medical Sciences
2015-2021
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is closely related to various cellular aspects associated with autophagy. However, how SARS-CoV-2 mediates the subversion of macroautophagy/autophagy pathway remains largely unclear. In this study, we demonstrate that overexpression ORF7a protein activates LC3-II and leads accumulation autophagosomes in multiple cell lines, while knockdown viral gene via shRNAs targeting sgRNA during decreased autophagy levels....
New emerging severe acute respiratory syndrome 2 (SARS-CoV-2) has caused a worldwide pandemic. Several animal models of coronavirus disease 2019 (COVID-19) have been developed and applied to antiviral research. In this study, two lethal mouse-adapted SARS-CoV-2 variants (BMA8 C57MA14) with different virulence were generated from hosts, which are characterized by high viral replication titers in the upper lower tract, pulmonary pathology, cytokine storm, cellular tropism, lymphopenia,...
Abstract Messenger RNA (mRNA) vaccine technology has shown its power in preventing the ongoing COVID-19 pandemic. Two mRNA vaccines targeting full-length S protein of SARS-CoV-2 have been authorized for emergency use. Recently, we developed a lipid nanoparticle-encapsulated (mRNA-LNP) encoding receptor-binding domain (RBD) (termed ARCoV), which confers complete protection mouse model. Herein, further characterized efficacy ARCoV nonhuman primates and long-term stability under normal...
Marburg virus disease (MVD) is a severe infectious characterized by fever and profound hemorrhage caused the (MARV), with mortality rate reaching 90%, posing significant threat to humans. MARV lies in its classification as biosafety level four (BSL-4) pathogen, which demands stringent experimental conditions substantial funding. Therefore, accessible practical animal models are urgently needed advance prophylactic therapeutic strategies for MARV. In this study, we constructed recombinant...
Middle East respiratory syndrome coronavirus (MERS-CoV), a new that has been causing severe and fatal acute illnesses in humans since its outbreak 2012, raised public fear worldwide. The development of prophylactics therapeutics is urgently needed to prevent control MERS-CoV infections. In this study, bacterium (Lactococcus lactis)-like particle (BLP) vaccine displaying the receptor-binding domain (RBD) was developed, gram-positive enhancer matrix (GEM) particles were used as substrates...
To date, intermediate hosts of SARS-CoV-2 remain obscure and controversial. Several studies have shown that SARS-CoV-2-related pangolin coronavirus (Pangolin-CoV) has a high sequence similarity to might be the initial source SARS-CoV-2; however, biological characteristics Pangolin-CoV are still largely unknown. In this study, we evaluated pathogenicity transmissibility in Syrian golden hamsters Mesocricetus auratus (Linnaeus, 1758) compared it with SARS-CoV-2. could effectively infect...
Previous studies have shown that B.1.351 and other variants extended the host range of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to mice. Sustained transmission is a prerequisite for viral maintenance in population. However, no evidence natural SARS-CoV-2 wild mice has been documented date. Here, we evaluated replication contact variant rats. The could infect replicate efficiently airways Furthermore, not be transmitted BALB/c or C57BL/6 but with moderate efficiency rats...
Wild aquatic birds are the primary hosts of H13 avian influenza viruses (AIVs). Herein, we performed a genetic analysis two AIVs isolated from wild in China and evaluated their infection potential poultry to further explore for transmission poultry. Our results showed that strains belong different groups, one strain (A/mallard/Dalian/DZ-137/2013; abbreviated as DZ137) belongs Group I, whereas other (A/Eurasian Curlew/Liaoning/ZH-385/2014; ZH385) III. In vitro experiments both DZ137 ZH385 can...
Abstract We recently detected a HKU4-related coronavirus in subgenus Merbecovirus (named pangolin-CoV-HKU4-P251T) from Malayan pangolin 1 . Here we report isolation and characterization of pangolin-CoV-HKU4-P251T, the genome sequence which is closest to that greater bamboo bat ( Tylonycteris robustula ) Yunnan Province, China, with 94.3% nucleotide identity. Pangolin-CoV-HKU4-P251T able infect human cell lines, replicates more efficiently cells express human-dipeptidyl-peptidase-4...
The Ebola virus (EBOV) is a member of the Orthoebolavirus genus, Filoviridae family, which causes severe hemorrhagic diseases in humans and non-human primates (NHPs), with case fatality rate up to 90%. development countermeasures against EBOV has been hindered by lack ideal animal models, as requires handling biosafety level (BSL)-4 facilities. Therefore, accessible convenient models are urgently needed promote prophylactic therapeutic approaches EBOV. In this study, recombinant vesicular...
We confirmed infection with influenza A(H1N1)pdm09 in giant pandas China during 2009 by using virus isolation and serologic analysis methods. This finding extends the host range of viruses indicates a need for increased surveillance control among pandas.
West Nile virus (WNV) causes a severe zoonosis, which can lead to large number of casualties and considerable economic losses. A rapid accurate identification methodfor WNV for use in field laboratories is urgently needed. Here, method utilizing reverse transcription loop-mediated isothermal amplification combined with vertical flow visualization strip (RT-LAMP-VF) was developed detect the envelope (E) gene WNV. The RT-LAMP-VF assay could 102 copies/μl ofan RNA standard using 40 min reaction...
Rift Valley fever virus (RVFV), which causes (RVF), is a mosquito-borne zoonotic pathogen that serious morbidity and mortality in livestock humans. RVF World Health Organization (WHO) priority disease and, together with rabies, major health burden Africa. Here, we present the development characterization of an inactivated recombinant RVFV rabies (RABV) vaccine candidate (rSRV9-eGn). Immunization rSRV9-eGn stimulated production RVFV-specific IgG antibodies induced humoral cellular immunity...
The emergence of Zika virus (ZIKV) infection, which is unexpectedly associated with congenital defects, has prompted the development safe and effective vaccines. Gram-positive enhancer matrix-protein anchor (GEM-PA) display system emerged as a versatile highly platform for delivering target proteins in In this study, we developed bacterium-like particle vaccine, ZI-△-PA-GEM, based on GEM-PA system. fusion protein ZI-△-PA, contains prM-E-△TM ZIKV (with stem-transmembrane region deletion) PA3,...
The weakened protective efficacy of COVID-19 vaccines and antibodies caused by SARS-CoV-2 variants presents a global health emergency, which underscores the urgent need for universal therapeutic antibody intervention clinical patients. Here, we screened three alpacas-derived nanobodies (Nbs) with neutralizing activity from twenty RBD-specific Nbs. Nbs were fused Fc domain human IgG, namely aVHH-11-Fc, aVHH-13-Fc aVHH-14-Fc, could specifically bind RBD protein competitively inhibit binding...