A5083375648- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- Long-Term Effects of COVID-19
- Virology and Viral Diseases
- Insect symbiosis and bacterial influences
- Mycobacterium research and diagnosis
- vaccines and immunoinformatics approaches
- Infectious Diseases and Mycology
- Malaria Research and Control
- Cancer-related molecular mechanisms research
- Animal Virus Infections Studies
- Viral-associated cancers and disorders
- Vaccine Coverage and Hesitancy
- SARS-CoV-2 detection and testing
- Birth, Development, and Health
- 3D Shape Modeling and Analysis
- Liver Disease Diagnosis and Treatment
- Lymphoma Diagnosis and Treatment
- Adrenal and Paraganglionic Tumors
- Gout, Hyperuricemia, Uric Acid
- Immunotherapy and Immune Responses
- Patient Safety and Medication Errors
- Tuberculosis Research and Epidemiology
Beijing Chest Hospital
2022-2024
Capital Medical University
2021-2024
Far Eastern University
2024
Xiangnan University
2023-2024
Academy of Military Medical Sciences
2019-2023
Institute of Microbiology
2017-2023
Wuhan Institute of Virology
2017-2023
Anhui Medical University
2017-2022
Jilin University
2020-2022
Beijing Children’s Hospital
2022
Mutation for microcephaly Zika virus infections in humans have been known since 1947. Microcephaly and neuropathologies associated with only reported recently, most prevalently the Americas. Yuan et al. investigated recent stable mutations genome engineered them into a low-virulence ancestral strain (see Perspective by Screaton Mongkolsapaya). A single amino acid substitution (serine to asparagine, S139N) viral precursor membrane protein exacerbated symptoms pregnant mice. The reverse...
The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has resulted in a global health emergency because of its rapid spread and high mortality. molecular mechanism interaction between host viral genomic RNA is yet unclear. We demonstrate herein that SARS-CoV-2 RNA, as well the negative-sense dynamically N6-methyladenosine (m6A)-modified human monkey cells. Combined RIP-seq miCLIP analyses total 8 m6A sites at single-base resolution genome. Especially, epidemic...
Abstract Messenger RNA (mRNA) vaccine technology has shown its power in preventing the ongoing COVID-19 pandemic. Two mRNA vaccines targeting full-length S protein of SARS-CoV-2 have been authorized for emergency use. Recently, we developed a lipid nanoparticle-encapsulated (mRNA-LNP) encoding receptor-binding domain (RBD) (termed ARCoV), which confers complete protection mouse model. Herein, further characterized efficacy ARCoV nonhuman primates and long-term stability under normal...
The high infection rate and rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) make it a world-wide pandemic. Individuals infected by the virus exhibited different degrees symptoms, most convalescent individuals have been shown to develop both cellular humoral immune responses. However, virus-specific adaptive responses in patients during phase not thoroughly studied. Here, we found that group COVID-19 with distress (ARDS) hospitalization, them mounted...
Safe and effective vaccines are urgently needed to end the COVID-19 pandemic caused by SARS-CoV-2 infection. We aimed assess preliminary safety, tolerability, immunogenicity of an mRNA vaccine ARCoV, which encodes spike protein receptor-binding domain (RBD).This single centre, double-blind, randomised, placebo-controlled, dose-escalation, phase 1 trial ARCoV was conducted at Shulan (Hangzhou) hospital in Hangzhou, Zhejiang province, China. Healthy adults aged 18-59 years negative for...
Zika virus (ZIKV) has caused significant outbreaks and epidemics in the Americas recently, raising global concern due to its ability cause microcephaly other neurological complications. A stable efficient infectious clone of ZIKV is urgently needed. However, instability toxicity flavivirus cDNA clones Escherichia coli hosts hindered development clones. Here, using a novel self-splicing ribozyme-based strategy, we generated contemporary strain imported from Venezuela China 2016. The...
Most mosquito-borne flaviviruses, including Zika virus (ZIKV), Dengue (DENV), and West Nile (WNV), produce long non-coding subgenomic RNAs (sfRNAs) in infected cells that link to pathogenicity immune evasion. Until now, the structural characterization of these lncRNAs remains limited. Here, we studied 3D structures individual combined subdomains sfRNAs, visualized accessible conformational spaces complete sfRNAs from DENV2, ZIKV, WNV by small angle X-ray scattering (SAXS) computational...
Abstract Human RNA binding protein Musashi-1 (MSI1) plays a critical role in neural progenitor cells (NPCs) by to various host transcripts. The canonical MSI1 site (MBS), A/GU (1-3) AG single-strand motif, is present many virus genomes, but only Zika (ZIKV) genome has been demonstrated bind MSI1. Herein, we identified the AUAG motif and AGAA tetraloop Xrn1-resistant 2 (xrRNA2) as non-canonical MBS, respectively, both are crucial for ZIKV neurotropism. More importantly, unique AGNN-type...
The combination of blueberry juice and probiotics reduces apoptosis alcoholic fatty liver mice by affecting SIRT1 pathway Juanjuan Zhu,1,2,* Tingting Ren,3,* Mingyu Zhou,2 Mingliang Cheng2 1First Hospital Affiliated to Suzhou University, Suzhou, 2Department Infectious Diseases, 3Biochemistry Department, Guiyang Medical College, Guiyang, People's Republic China *These authors contributed equally this work Purpose: To explore the effects on disease (AFLD).Methods: Healthy C57BL/6J were used in...
Zika virus (ZIKV) can infect a wide range of tissues including the developmental brain human fetus. Whether specific viral genetic variants are linked to neuropathology is incompletely understood. To address this, we have intracranially serially passaged clinical ZIKV isolate (SW01) in neonatal mice and discovered that exhibit markedly increased virulence neurotropism. Deep sequencing analysis combining with molecular virology studies revealed single 67D (Aspartic acid) N (Asparagine)...
As the task of 2D-to-3D reconstruction has gained significant attention in various real-world scenarios, it becomes crucial to be able generate high-quality point clouds. Despite recent success deep learning models generating clouds, there are still challenges producing high-fidelity results due disparities between images and While vision transformers (ViT) diffusion have shown promise tasks, their benefits for reconstructing clouds from not been demonstrated yet. In this paper, we first...
Infections caused by rapidly growing mycobacteria (RGM) have increased globally. Chemotherapy against these infections is challenging due to the minimal antimicrobial choices available. The main aim of this study was evaluate in vitro susceptibilities four tetracyclines different RGM species. MICs eravacycline (ERC), omadacycline (OMC), sarecycline (SAC), and tigecycline (TGC) reference strains 27 species 121 clinical isolates were determined microtiter plate assay. minimum bactericidal...
Abstract Increasing evidence shows the African lineage Zika virus (ZIKV) displays a more severe neurovirulence compared to Asian ZIKV. However, viral determinants and underlying mechanisms of enhanced virulence phenotype remain largely unknown. Herein, we identify panel amino acid substitutions that are unique ZIKVs by phylogenetic analysis sequence alignment. We then utilize reverse genetic technology generate recombinant incorporating these lineage-specific based on an infectious cDNA...
SUMMARY The World Health Organization has declared SARS-CoV-2 virus outbreak a world-wide pandemic. Individuals infected by the exhibited different degrees of symptoms, basis which remains largely unclear. Currently, though convalescent individuals have been shown with both cellular and humoral immune responses, there is very limited understanding on especially adaptive in patients severe COVID-19. Here, we examined 10 blood samples from COVID-19 acute respiratory distress syndrome (ARDS)....
Human immunodeficiency virus (HIV) significantly increases the risk of non-Hodgkin lymphoma (NHL) development, yet population-level impact on NHL burden is unquantified. We aim to quantify this association and estimate global HIV-associated NHL.In meta-analysis, we searched five databases (PubMed, EMBASE, Cochrane Library, Web Science, Scopus) from database inception up September 13, 2023, identifying cohort, case-control, or cross-sectional studies with an effective control group assess...
Safe and effective vaccines therapeutics based on the understanding of antiviral immunity are urgently needed to end COVID-19 pandemic. However, these immune responses, especially cellular responses SARS-CoV-2 infection, is limited. Here, we conducted a cohort study patients who were followed had blood collected characterize longitudinal dynamics their responses. Compared with healthy controls, percentage activation S/N-specific T cells in recovered was significantly higher. And CD8 + higher...
Background: Although the prevalence of hypertension has been well studied in middle age and elderly populations, few studies have systematically investigated its association with cardiovascular cerebrovascular risk factors young populations.Objective: This study examined college students correlation factors, such as neck circumference body mass index (BMI).Methods: population-based recruited a total 1719 (723 were junior, 502 sophomore, 494 freshman), including 996 males (average age: 20.8...
Zika virus (ZIKV) has been identified as a cause of microcephaly and acquired evolutionary mutations since its discovery. Previously deletions in the E glycan loop were recorded African ZIKV strains, which have re-emerged contemporary Asian lineages recently.
This paper introduces a mental health status assessment system based on the Logistic regression model. The mainly consists of user end, server database, web server, and client. Users can assess their through browser or mobile terminal, record query results. end accesses database via displays results in page format. To ensure secure storage transmission information, design also fully considers privacy protection data security. uses model as its core algorithm, combining users' personal...