A5074152562- Plant nutrient uptake and metabolism
- Plant Micronutrient Interactions and Effects
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Arsenic contamination and mitigation
- Legume Nitrogen Fixing Symbiosis
- Aluminum toxicity and tolerance in plants and animals
- Photosynthetic Processes and Mechanisms
- Metalloenzymes and iron-sulfur proteins
- Plant Reproductive Biology
- Nitrogen and Sulfur Effects on Brassica
- Mitochondrial Function and Pathology
- Polysaccharides and Plant Cell Walls
- Plant Pathogenic Bacteria Studies
- Iron oxide chemistry and applications
- Plant Disease Resistance and Genetics
- Pesticide and Herbicide Environmental Studies
- Light effects on plants
- Chromosomal and Genetic Variations
- Plant Pathogens and Fungal Diseases
- Fungal and yeast genetics research
- Phytase and its Applications
- Glutathione Transferases and Polymorphisms
- Carbon and Quantum Dots Applications
- Metabolism, Diabetes, and Cancer
Zhejiang University
2013-2025
State Key Laboratory of Plant Physiology and Biochemistry
2009-2023
Nanjing Agricultural University
2015
Rothamsted Research
2011
Oxford Brookes University
2011
University of Oxford
2011
China National Rice Research Institute
2005
Abstract Previous research has demonstrated that AtPHR1 plays a central role in phosphate (Pi)-starvation signaling Arabidopsis thaliana. In this work, two OsPHR genes from rice (Oryza sativa) were isolated and designated as OsPHR1 OsPHR2 based on amino acid sequence homology to AtPHR1. Their functions Pi investigated using transgenic plants. Our results showed both are involved Pi-starvation pathway by regulation of the expression Pi-starvation-induced genes, whereas only overexpression...
Significance Phosphate (Pi) is a primary nutrient for plant growth. Because of the low availability soil Pi, Pi starvation signaling in plants gaining great interest. Arabidopsis AtPHR1 and its rice homologue OsPHR2 are known to be central transcription factors homeostasis; however, mechanism how sense external fluctuation regulate activity AtPHR1/OsPHR2 has been elusive. Here, we identify SPX1 SPX2 as Pi-dependent inhibitors PHR2, implicating Pi-sensing mechanism. We also show that SPX...
Abstract We report here on a novel transcription factor with basic helix-loop-helix domain for tolerance to inorganic phosphate (Pi) starvation in rice (Oryza sativa). The gene is designated OsPTF1. expression of OsPTF1 Pi induced roots while constitutively expressed shoots, as shown by northern-blot analysis. Overexpression enhanced transgenic rice. Tillering ability, root and shoot biomass, phosphorus content plants were about 30% higher than those the wild-type Pi-deficient conditions...
Abstract Arsenic (As) accumulation in rice (Oryza sativa) may pose a significant health risk to consumers. Plants take up different As species using various pathways. Here, we investigated the contribution of phosphate (Pi) transport pathway grown hydroponically or under flooded soil conditions. In hydroponic experiments, mutant defective OsPHF1 (for transporter traffic facilitator1) lost much ability Pi and arsenate them from roots shoots, whereas transgenic overexpressing either OsPht1;8...
PHR2, a central regulator of phosphate signaling in rice, enhanced the expression starvation-induced (PSI) genes and resulted enhancement Pi acquisition under deficiency stress. This occurred via PHR2 binding to cis-element named PHR1 sequences. However, transcription level was not responsive starvation. So how is activity factor adjusted adapt diverse status? Here, we identify an SPX family protein, Os-SPX4 (SPX4 hereafter), involving starvation acting as negative PHR2. SPX4 shown be fast...
Rice is a major dietary source of the toxic metalloid arsenic (As). Reducing its accumulation in rice (Oryza sativa) grain critical importance to food safety. roots take up arsenate and arsenite depending on prevailing soil conditions. The first step detoxification reduction arsenite, but enzyme(s) catalyzing this reaction remains unknown. Here, we identify OsHAC1;1 OsHAC1;2 as reductases rice. are able complement an Escherichia coli mutant lacking endogenous reductase reduce arsenite....
Abstract Phosphorus (P), an essential macronutrient for all living cells, is indispensable agricultural production. Although Arabidopsis (Arabidopsis thaliana) PHOSPHATE RESPONSE1 (PHR1) and its orthologs in other species have been shown to function transcriptional regulation of phosphate (Pi) signaling Pi homeostasis, integrative comparison PHR1-related proteins rice (Oryza sativa) has not previously reported. Here, we identified functional redundancy among three PHR1 (OsPHR1, OsPHR2,...
Soil contamination with arsenic (As) can cause phytotoxicity and elevated As accumulation in rice grain. Here, we used a forward genetics approach to investigate the mechanism of arsenate (As(V)) tolerance rice. A mutant hypersensitive As(V), but not As(III), was isolated. Genomic resequencing complementation tests were identify causal gene. The function gene, its expression pattern subcellular localization characterized. OsHAC4 is gene for As(V)-hypersensitive phenotype. encodes...
PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1 (PHF1) is known to regulate the plasma membrane localization of PHT1;1, a high-affinity inorganic phosphate (Pi) transporter in Arabidopsis (Arabidopsis thaliana). OsPHF1, rice (Oryza sativa) gene homologous AtPHF1, was isolated and found both low- Pi transporters membrane. Three OsPHF1 allelic mutants carrying one-point mutations at fifth WD-repeat motif two transmembrane helix, respectively, showed arsenate resistance severely reduced...
Abstract We characterized the function of two rice phosphate ( P i) transporters: OsPHT1 ;9 OsPT9 ) and ;10 OsPT10 ). were expressed in root epidermis, hairs lateral roots, with their expression being specifically induced by i starvation. In leaves, genes was observed both mesophyll vasculature. High‐affinity K m values for transport determined yeast experiments two‐electrode voltage clamp analysis anion X enopus oocytes expressing . uptake concentrations transgenic plants harbouring...
Phosphate transporters (PTs) mediate phosphorus uptake and are regulated at the transcriptional posttranslational levels. In one key mechanism of regulation, phosphorylation PTs affects their trafficking from endoplasmic reticulum (ER) to plasma membrane. However, kinase(s) mediating PT leading ER retention phosphorylated remain unclear. this study, we identified a rice (Oryza sativa) kinase subunit, CK2β3, which interacts with PT2 PT8 in yeast two-hybrid screen. Also, CK2α3/β3 holoenzyme...
Abstract Rice (Oryza sativa) takes up arsenite mainly through the silicic acid transport pathway. Understanding uptake and sequestration of arsenic (As) into rice plant is important for developing strategies to reduce As concentration in grain. In this study, cellular subcellular distributions silicon (Si) roots were investigated using high-pressure freezing, high-resolution secondary ion mass spectrometry, transmission electron microscopy. plants, both lsi2 mutant lacking Si/arsenite efflux...
Root system architecture is very important for plant growth and crop yield. It essential nutrient water uptake, anchoring, mechanical support. angle (RGA) a vital constituent of root used as parameter variety evaluation in breeding. However, little known about the underlying molecular mechanisms that determine rice (Oryza sativa). In this study, mutant large angle1 (lra1) was isolated shown to exhibit RGA reduced sensitivity gravity. Genome resequencing complementation assays identified...
Summary Arsenic (As) contamination in a paddy environment can cause phytotoxicity and elevated As accumulation rice ( Oryza sativa ). The mechanism of detoxification is still poorly understood. We isolated an arsenate (As(V))‐sensitive mutant rice. Genomic resequencing complementation identified OsCLT1 , encoding CRT‐like transporter, as the causal gene for phenotype. localized to envelope membrane plastids. glutathione γ‐glutamylcysteine contents roots Osclt1 RNA interference lines were...
Ideal root system architecture (RSA) is important for efficient nutrient uptake and high yield in crops. We cloned characterized a key RSA regulatory gene, GRAVITROPISM LOSS 1 (OsGLS1), rice (Oryza sativa L.). The gls1 mutant displays an increased growth angle, longer primary roots, more adventitious roots greater efficiency grain paddy fields. OsGLS1 strongly expressed the tips of seedlings leaves at flowering stage. encodes RING finger E3 ubiquitin ligase mainly localizing basal plasma...
The rice (Oryza sativa L.) genome contains at least six genes exclusively with an SPX (SYG1/PHO81/XPR1) domain the N-terminal, designated as OsSPX1-6. Here we report diverse expression patterns of OsSPX in different tissues and their responses to Pi-starvation. Among them, five genes, OsSPX1, 2, 3, 5 6 are responsive Pi-starvation shoots and/or roots. subcellular localization analysis indicates that OsSPX1 OsSPX2 is located nucleus, OsSPX3 cytoplasm, OsSPX4 a membrane protein. regulates...
The importance of SPX-domain-containing proteins to phosphate (Pi) homeostasis and signalling transduction has been established in plants. In this study, phylogenetic analysis revealed that OsSPX3 OsSPX5 (SPX3/5) are paralogous SPX genes (SYG1/Pho81/XPR1) cereal crops. SPX3/5 specifically responsive Pi starvation at both the transcriptional post-transcriptional levels. Similar tissue expression patterns two were identified by situ hybridization transgenic plants harbouring SPX3pro-SPX3-GUS...
Phosphate (Pi) uptake in plants depends on plasma membrane (PM)-localized phosphate transporters (PTs). OsCK2 phosphorylates PTs and inhibits their trafficking from the endoplasmic reticulum (ER) to PM rice (Oryza sativa), but how are dephosphorylated is unknown. We demonstrate that protein phosphatase type 2C (PP2C) OsPP95 interacts with OsPT2 OsPT8 dephosphorylates at Ser-517. Rice overexpressing reduced phosphorylation promoted ER PM, resulting Pi accumulation. Under Pi-sufficient...
ABSTRACT The involvement of cytokinins (CTKs) in the repression phosphate (Pi)‐starvation signalling has been widely documented. However, full physiological and molecular relevance this role remains unclear. To gain further insights into regulation system CTK Pi‐starvation signalling, a global analysis gene expression events rice seedlings under Pi starvation, exogenous treatment Pi‐sufficient (+P) Pi‐deficient (−P) conditions, was conducted using oligonucleotide array analysis....
The origin recognition complex (ORC) is a pivotal element in DNA replication, heterochromatin assembly, checkpoint regulation and chromosome assembly. Although the functions of ORC have been determined yeast model animals, they remain largely unknown plant kingdom. In this study, Oryza sativa Origin Recognition Complex subunit 3 (OsORC3) was cloned using map-based cloning procedures, functionally characterized rice (Oryza sativa) orc3 mutant. mutant showed temperature-dependent defect...
• A rice mutant, Oryza sativa short postembryonic roots 1 (Osspr1), has been characterized. It roots, including adventitious and lateral a lower iron content in its leaves. OsSPR1 was identified by map-based cloning. encodes novel mitochondrial protein with the Armadillo-like repeat domain. Osspr1 mutants exhibited decreased root cell elongation. The of mutant shoots significantly altered compared that wild-type shoots. similar pattern alteration manganese zinc concentrations also observed....
LIR1 (LIGHT-INDUCED RICE1) encodes a 13-kD, chloroplast-targeted protein containing two nearly identical motifs of unknown function. is present in the genomes vascular plants, mosses, liverworts, and algae, but not cyanobacteria. Using coimmunoprecipitation assays, pull-down yeast two-hybrid analyses, we showed that interacts with LEAF-TYPE FERREDOXIN-NADP+ OXIDOREDUCTASE (LFNR), an essential chloroplast enzyme functioning last step photosynthetic linear electron transfer. LFNR formed high...