A5072111193- Glioma Diagnosis and Treatment
- CAR-T cell therapy research
- Autophagy in Disease and Therapy
- RNA modifications and cancer
- Neuroblastoma Research and Treatments
- Ubiquitin and proteasome pathways
- Chromatin Remodeling and Cancer
- Protein Degradation and Inhibitors
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Endoplasmic Reticulum Stress and Disease
- Growth Hormone and Insulin-like Growth Factors
- Pituitary Gland Disorders and Treatments
- Cancer Genomics and Diagnostics
- Cannabis and Cannabinoid Research
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Genetic factors in colorectal cancer
- Monoclonal and Polyclonal Antibodies Research
- ATP Synthase and ATPases Research
- Immunotherapy and Immune Responses
- Hippo pathway signaling and YAP/TAZ
- Brain Metastases and Treatment
- Phagocytosis and Immune Regulation
- Telomeres, Telomerase, and Senescence
Children Cancer Hospital
2015-2024
University of Louisville
2007-2009
Target (United States)
2008-2009
James Graham Brown Foundation
2008-2009
Overexpression of zinc finger E-box binding homeobox transcription factor 1 (Zeb1) in cancer leads to epithelial-to-mesenchymal transition (EMT) and increased metastasis. As opposed overexpression, we show that mutation Zeb1 mice causes a mesenchymal-epithelial gene expression characterized by ectopic epithelial genes such as E-cadherin loss mesenchymal vimentin. In contrast rapid proliferation cells where is overexpressed, this mutant associated with diminished progenitor at sites...
Abstract Pediatric high-grade gliomas (HGG) are rare aggressive tumors that present a prognostic and therapeutic challenge. Diffuse midline glioma, H3K27M–mutant is new entity introduced to HGG in the latest WHO classification. In this study we evaluated presence of H3K27M mutation 105 tumor samples histologically classified into low-grade (LGG) (n = 45), 60). Samples were screened for histone H3.3 H3.1 variants examine its prevalence, impact, assess potential clinical value limited resource...
Atk can be activated by two independent phosphorylation events. Growth factor-dependent of threonine 308 (Akt-308) phosphatidylinositol 3-kinase-dependent PDK1 leads to activation mammalian target rapamycin (mTor) complex 1 (TORC1) and stimulation protein synthesis. Phosphorylation on serine 473 (Akt-473) is catalyzed mTor in a second (TORC2), Akt-473 phosphorylates Foxo3a inhibit apoptosis. Accumulation both phosphorylated forms Akt frequent cancer, TORC2 activity required for progression...
Ovarian epithelial cancer is a significant cause of death among women, accounting for 5% all female cancer-related fatalities. A lack reliable detection methods and resistance to chemotherapy agents are considerable obstacles in the treatment this cancer. Recently, high-level expression pituitary tumor transforming gene (PTTG) was found wide range tumors, including ovarian cancers. Elevated PTTG levels were induce cellular transformation vitro formation nude mice. Therefore, we hypothesize...
Aggresomes are transient microtubule-dependent inclusion bodies that sequester misfolded proteins and ultimately removed by autophagy. Here we report the generation of a choroid plexus carcinoma cell line; Children's Cancer Hospital Egypt (CCHE)-45, which is characterized constitutive formation aggresomes. When examining autophagy pathway as main route for aggresomes clearance, CCHE-45 cells displayed increased flux mediated MAP1LC3B. MAP1LC3A-Variant1 gene expression was silenced promoter...
Histone deacetylase 6 (HDAC6) is an attractive target for cancer therapeutic intervention. Selective HDAC6 inhibitors important to minimise the side effects of pan inhibition. Thus, new class hydroxamic acid-based derivatives were designed on structural basis perform preferential activity against targeting solid tumours. Interestingly, 1-benzylbenzimidazole-2-thio-N-hydroxybutanamide 10a showed impressive preference with submicromolar potency (IC50 = 510 nM). cytotoxic interesting profile...
Disruption of the Rb (retinoblastoma protein)/E2F cell-cycle pathway and Ras activation are two most frequent events in cancer, both these mutations place oncogenic stress on cells to increase DNA replication. In present study, we demonstrate that have an additive effect induction members RecQ helicase family. activity is important for genomic stability, initiation replication telomere maintenance, mutation BLM (Bloom's syndrome gene), WRN (Werner's gene) or RECQL4 (Rothmund-Thomson family...
The pituitary tumor transforming gene ( PTTG )/securin is an oncogene that involved in cell cycle regulation and sister chromatid separation. highly expressed various tumors including ovarian tumors, suggesting may play a role tumorigenesis. Overexpression of resulted induction cellular transformation vitro formation nude mice. To ascertain function tumorigenesis, we generated transgenic mouse model by cloning cDNA downstream Mullerian inhibitory substance type II receptor promoter MISIIR )...
Osteosarcoma is a primary malignant bone tumor affecting adolescents and young adults. This study aimed to identify proteomic signatures that distinguish between different osteosarcoma subtypes, providing insights into their molecular heterogeneity potential implications for personalized treatment approaches. Using advanced techniques, we analyzed FFPE samples from cohort of pediatric patients representing four various subtypes. Differential expression analysis revealed significant signature...
Aggresomes are inclusion bodies for misfolded/aggregated proteins. Despite the role of proteins in neurological disorders, their cancer pathogenesis is poorly defined. In current study we aimed to investigate whether aggresomes-positivity could be used improve disease subclassification and prognosis prediction pediatric medulloblastoma. Ninety three medulloblastoma tumor samples were retrospectively stratified into molecular subgroups; WNT, SHH non-WNT/non-SHH, using immunohistochemistry...
Protein misfolding and aggregation result in proteotoxic stress underlie the pathogenesis of many diseases. To overcome proteotoxicity, cells compartmentalize misfolded aggregated proteins different inclusion bodies. The aggresome is a paranuclear body that functions as storage compartment for proteins. Choroid plexus tumors (CPTs) are rare neoplasms comprised three pathological subgroups. underlying mechanisms their remain unclear. This study aims to elucidate prognostic role biological...
ImmunotherapyVol. 7, No. 5 CommentaryArmed hunter killers: discerning the role of adoptive T-cell transfer for glioblastomaHeba Samaha, Shahenda El Naggar & Nabil AhmedHeba SamahaAuthor correspondence: E-mail Address: heba.samaha@57357.orgBasic Research Department Children's Cancer Hospital Egypt (CCHE 57357), 1 Seket el Emam, Sayeda Zeinab, Cairo, Egypt, 11441, NaggarBasic 11441 AhmedCenter Cell Gene Therapy, Texas Hospital, Houston Methodist Houston, TX, USATexas Hematology Centers,...
The unfolded protein response pathways (UPR), autophagy, and compartmentalization of misfolded proteins into inclusion bodies are critical components the quality control network. Among bodies, aggresomes particularly intriguing due to their association with cellular survival, drug resistance, aggresive cancer behavior. Aggresomes molecular condensates formed when collapsed vimentin cages encircle before final removal by autophagy. Yet significant gaps persist in mechanisms governing...
Embryonal tumor with multilayered rosettes (ETMR) is an aggressive and rare pediatric embryonal brain tumor. Amplification of C19MC microRNA cluster expression LIN28 are distinctive features ETMR. Despite the increasing efforts to decipher ETMR, biology remains poorly understood. To date, role aberrant alternative splicing in ETMR has not been thoroughly investigated. In current study, a comprehensive analysis was performed on published unprocessed RNA-seq reads tissue-matched fetal controls...
Abstract Our long-term goal is to define and characterize the mechanisms underlying aggresome formation in choroid plexus carcinoma a rare pediatric tumor. Protein misfolding inevitable, 30% of newly synthesized polypeptides can end up misfolded, such proteins are either refolded or eliminated by cellular quality control pathways which include ubquitin proteosome system autophagy. In recent years, protein has been implicated pathophysiology many diseases as diabetes, neurological disorders...
Abstract Purpose High-grade gliomas (HGG) in infants and very young children (less than 3 to 5 years old) pose significant challenges due the limited scientific literature available high risks associated with treatments. This study aims investigate characteristics, treatment, outcomes of this cohort. Methods A retrospective cohort was conducted from 2007 2022 at Children’s Cancer Hospital, Egypt (CCHE-57357). Cases included aged < old pathologically confirmed CNS glioma grades III-IV....