A5064192946- Antimicrobial Peptides and Activities
- Antifungal resistance and susceptibility
- Biochemical and Structural Characterization
- Monoclonal and Polyclonal Antibodies Research
- Transgenic Plants and Applications
- Oral microbiology and periodontitis research
- Protein Hydrolysis and Bioactive Peptides
- Gut microbiota and health
- Probiotics and Fermented Foods
- Salivary Gland Disorders and Functions
- Herpesvirus Infections and Treatments
- Fungal Infections and Studies
- Photodynamic Therapy Research Studies
- Insect and Pesticide Research
- Toxoplasma gondii Research Studies
- Immunotherapy and Immune Responses
- Endodontics and Root Canal Treatments
- Toxin Mechanisms and Immunotoxins
- Mycotoxins in Agriculture and Food
- Immune Cell Function and Interaction
- Nanoplatforms for cancer theranostics
- Glycosylation and Glycoproteins Research
- Fungal and yeast genetics research
- Invertebrate Immune Response Mechanisms
- Bacteriophages and microbial interactions
University of Parma
2015-2024
Synthetic peptides with sequences identical to fragments of the constant region different classes (IgG, IgM, IgA) antibodies (Fc-peptides) exerted a fungicidal activity in vitro against pathogenic yeasts, such as Candida albicans, glabrata, Cryptococcus neoformans, and Malassezia furfur, including caspofungin triazole resistant strains. Alanine-substituted derivatives Fc-peptides, tested evaluate critical role each residue, displayed unaltered, increased or decreased candidacidal vitro. An...
It has been documented that, independently from the specificity of native antibody (Ab) for a given antigen (Ag), complementarity determining regions (CDR)-related peptides may display differential antimicrobial, antiviral and antitumor activities.In this study we demonstrate that synthetic peptide with sequence identical to V(H)CDR3 mouse monoclonal Ab (mAb) specific difucosyl human blood group A is easily taken up by macrophages subsequent stimulation of: i) proinflammatory cytokine...
<b><i>Background/Aims:</i></b> New antivirals are needed to supplement or replace currently used drugs. The aim of this study was evaluate the antiviral activity synthetic peptides derived from physiological proteins. <b><i>Methods:</i></b> Vero cell monolayers were infected with herpes simplex virus 1, vesicular stomatitis virus, adenovirus, and coxsackievirus B5 strains in presence different concentrations selected viral yield determined by...
The detection of salivary molecules associated with pathological and physiological alterations has encouraged the search novel non-invasive diagnostic biomarkers for oral health evaluation. While genomic, transcriptomic, proteomic profiles human saliva have been reported, its metabolic composition is a topic research: metabolites in submandibular/sublingual never analyzed systematically. In this study, samples whole, parotid, from 20 healthy donors, without dental or periodontal diseases,...
The ubiquitous commensal Candida albicans, part of the human microbiota, is an opportunistic pathogen able to cause a wide range diseases, from cutaneous mycoses life-threatening infections in immunocompromised patients. albicans adapts different environments and survives long-time starvation. ability switch yeast hyphal morphology under specific environmental conditions associated with its virulence. Using hydrogen nuclear magnetic resonance spectroscopy, we profiled intracellular...
In cells derived from several types of cancer, a transcriptional program drives high consumption glutamine (Gln), which is used for anaplerosis, leading to metabolic addiction the amino acid. Low or absent expression Glutamine Synthetase (GS), only enzyme that catalyzes de novo Gln synthesis, has been considered marker Gln-addicted cancers. this study, two human cell lines brain tumors with oligodendroglioma features, HOG and Hs683, have shown be GS-negative. Viability both depends...
The killer peptide KP is a synthetic decapeptide derived from the sequence of variable region recombinant yeast toxin-like microbicidal single-chain antibody. proved to exert significant activities against diverse microbial and viral pathogens through different mechanisms action, but little known its effect on apicomplexan protozoa. aim present study was evaluate in vitro activity Toxoplasma gondii, globally widespread protozoan parasite great medical interest. treatment potential mechanism...
The aim of the study is to test application different laser wavelengths, with and without photosensitizing dyes on types cultures. Laser irradiation was realized
Antimicrobial resistance is a major public health concern worldwide. Albeit to lesser extent than bacteria, fungi are also becoming increasingly resistant antifungal drugs. Moreover, due the small number of classes, therapy options limited, complicating clinical management mycoses. In this view, antimicrobial peptides (AMPs) potential alternative conventional Among these, Proline-rich (PrAMPs), almost exclusively animal origins, particular interest their peculiar mode action. study, search...
Fungal infections still represent relevant human illnesses worldwide and some are accompanied by unacceptably high mortality rates. The limited current availability of effective safe antifungal agents makes the development new drugs approaches vaccination/immunotherapy every day more needed. Among them, small antibody(Ab)-derived peptides arousing great expectations as potential agents. In this topic, search path from study yeast killer phenomenon to production Ab-derived characterized in...
It was previously reported that injection of anaflatoxin B1 (AnAFB1) conjugated to keyhole limpet hemocyanin (KLH), together with Freund's adjuvant, effective in inducing cows a long lasting titer anti-aflatoxin (AFB1) antibodies (Abs), cross-reacting other aflatoxins, which were able hinder, proportionally their titer, the secretion aflatoxin M1 (AFM1) into milk continuously fed AFB1. According anti-AFB1 Ab 50% vaccinated recognized as high responder animals. In an attempt prepare more...
The human organism is inhabited by trillions of microorganisms, known as microbiota, which are considered to exploit a pivotal role in the regulation host health and immunity. Recent investigations have suggested relationship between composition microbiota COVID-19 infection, highlighting possible bacterial communities modulation disease severity. In this study, we performed shotgun metagenomics analysis explore compare nasopharyngeal 38 hospitalized Italian patients with without infection...
We previously reported that a synthetic peptide with sequence identical to CDR of mouse monoclonal antibody specific for difucosyl human blood group A exerted an immunomodulatory activity on murine macrophages. It was therapeutic against systemic candidiasis without possessing direct candidacidal properties. Here we demonstrate selected peptide, N10K, putatively deriving from the enzymatic cleavage constant region (Fc) IgG1, is able induce IL-6 secretion and pIkB-α activation. More...
Evidence from previous works disclosed the antimicrobial, antiviral, anti-tumour and/or immunomodulatory activity exerted, through different mechanisms of action, by peptides expressed in complementarity-determining regions or even constant region antibodies, independently their specificity and isotype. Presently, we report selection, available databases, peptide sequences encoded immunoglobulin genes for evaluation potential biological activities. Synthetic representing translated products...
In order to overcome the limitations inherent in current pharmacological treatments for Malassezia pachydermatis, cause of otitis externa dogs, efficacy a killer decapeptide (KP) was evaluated vitro and vivo. Sixteen dogs with naturally occurring M. pachydermatis were enrolled, fungicidal activity KP using yeasts recovered from these animals. The therapeutic four groups animals each. topically treated (150 μl, 2 mg/ml) three times per week (group A) or every day B), scramble peptide C), left...