This study evaluated the cytotoxicity activity of essential oils Tagetes erecta L., Asteraceae (TE-OE), Tetradenia riparia (Hochst.) Codd, Lamiaceae (TR-OE), Bidens sulphurea (Cav.) Sch. Bip., (BS-OE), and Foeniculum vulgare Mill., Apiaceae (FV-OE), traditionally used in folk medicine, against tumor cell lines murine melanoma (B16F10), human colon carcinoma (HT29), breast adenocarcinoma (MCF-7), cervical (HeLa), hepatocellular liver (HepG2), glioblastoma (MO59J, U343, U251). Normal hamster...

Dental prosthesis supports Candida species growth and may predispose the oral cavity to lesions. C. tropicalis has emerged as a colonizer of shown resistance clinically used antifungal agents, which increased search for new antifungals. This work describes effectiveness fifteen essential oils (EOs) against The EOs were obtained by hydrodistillation chemically characterized gas chromatography-mass spectrometry. activities evaluated microdilution method showed that Pelargonium graveolens...

Description of the subject. Essential oils are volatile fractions produced through special metabolism plants. One significant biological application essential in recent years has been their use as bioherbicides, due to important biocide effect. Objectives. This study aims identify chemical constituents Aloysia gratissima (Gillies & Hook.) Tronc. oil and assess its potential. Method. describes, for first time, constitution from seeds A. gratissima. To verify possible effect, lettuce were...

In this work, the primary objective was to achieve a complete and detailed structural assignment of 1H 13C nuclear magnetic resonance (NMR) data for kaurenoic acid (KA) (ent-kaur-16-en-19-oic acid), widely studied natural diterpene with various reported biological activities. To facilitate this, methyl ester (ent-methyl-kaur-16-en-19-oate) also elucidated, leveraging its slight differences corresponding variations in chemical shifts. The comprehensive accomplished through analysis spectra...

This work used the broth microdilution method to investigate antimicrobial activity of essential oil obtained from leaves Plectranthus neochilus (PN-EO) against a representative panel oral pathogens. We assessed this in terms minimum inhibitory concentration (MIC). PN-EO displayed moderate Enterococcus faecalis (MIC = 250 μ g/mL) and Streptococcus salivarus g/mL), significant sobrinus 62.5 sanguinis mitis 31.25 Lactobacillus casei interesting mutans 3.9 g/mL). GC-FID GC-MS helped identify...

The in vitro schistosomicidal effects of the essential oil obtained from Tagetes erecta L. Asteraceae, leaves (TE-EO) collected Brazil against Schistosoma mansoni worms are reported this paper. caused a significant decrease motor activity at 50 µg/mL as minimal concentration after 24 h. This also death all parasites and separation coupled pairs into individual male female 100 viability adult worm groups treated with TE-EO was similar to that praziquantel (positive control). In addition,...

We have investigated the chemical composition and antibacterial activity of essential oil Dysphania ambrosioides (L.) Mosyakin & Clemants (Chenopodiaceae) ( DA ‐ EO ) against a representative panel cariogenic bacteria. also assessed in vitro schistosomicidal effects on Schistosoma mansoni its cytotoxicity to GM 07492‐A cells . Gas chromatography GC gas chromatography‐mass spectrometry / MS revealed that monoterpenes cis ‐piperitone oxide (35.2%), p ‐cymene (14.5%), isoascaridole (14.1%),...

This study reports on the chemical composition and antimicrobial activity of essential oil from Artemisia absinthium L. leaves AA EO collected in Brazil against a representative panel cariogenic bacteria. The minimum inhibitory concentrations MICs were determined by broth dilution method 96-well microplates. was inactive most investigated oral pathogens, but it displayed moderate Streptococcus mutans MIC = 250 μg/mL very promising mitis 62.5 μg/mL. Gas chromatography GC FID gas mass...

Even though essential oils from Piper aduncum (Piperaceae) have different biological activities, little is known about their application to agricultural areas. White mold a plant disease caused by the phytopathogen Sclerotinia sclerotiorum, which needs be controlled alternative measures. This study aimed at evaluating effect of P. leaves (PL-EO) and inflorescences (PI-EO) on mycelial growth S. sclerotiorum. Essential were obtained hydrodistillation Clevenger-type apparatus while chemical...

The antibacterial activity of nine selected essential oils (EOs) against a panel oral pathogens was investigated in terms their minimum inhibitory concentrations (MICs) by using the broth microdilution method. Most EOs displayed weak or were inactive pathogens, with MIC values ranging from 500 to 4000 μg/mL. However, EO obtained leaves Bidens sulphurea (Asteraceae) found display moderate Streptococcus mutans (MIC = 250 μg/mL) and significant mitis 31.25 μg/mL). Germacrene D (38.3%),...

Chagas disease is a neglected tropical that affects more than 8 million people. Although there are therapies against this disease, the search for new drugs important because current treatments show limited effectiveness and high toxicity. In work, eighteen dihydrobenzofuran-type neolignans (DBNs) two benzofuran-type (BNs) were synthesized evaluated amastigote forms of Trypanosoma cruzi strains. The in vitro cytotoxicity hemolytic activity most active compounds also their relationships with...

In this study, the chemical composition and in vitro schistosomicidal properties of essential oil obtained from Bidens sulphurea flowers (Bs-EO) were investigated. Its major constituents identified as being 2,6-di-tert-butyl-4-methylphenol (44.98%), germacrene D (33.70%) β-caryophyllene (10.23%). Bs-EO at 100 µg mL−1 caused death all adult worms promoted separation couple pairs into individual male female within 48 h, besides leading to a significant decrease motility parasites. This was...

We report the in vitro schistosomicidal effects of essential oil obtained from Citrus limonia leaves (CL-EO) and C. reticulata fruit peels (CR-EO), cultivated Brazil, against Schistosoma mansoni worms. Limonene (29.9%), β-pinene (12.0%), sabinene (9.0%), citronellal citronellol (5.8%) are major constituents CL-EO; limonene (26.5%), γ-terpinene (17.2%), linalool (11.1%), octanal (8.0%), myrcene (6.2%), capraldehyde (3.9%) predominate CR-EO. CL-EO displayed moderate lethal concentration 50%...

Copaifera (Leguminoseae) species produce a commercially interesting oleoresin that displays several biological activities, including antimicrobial and anti-inflammatory properties. Labdane-type diterpenes are the main chemical constituents of these oleoresins, copalic acid is only compound has been detected in all oleoresins. In this study, we investigate some aspects gas-phase fragmentation reactions involved formation product ions from deprotonated compounds (-)-ent-copalic (1),...

We investigated the gas-phase fragmentation reactions of a series 2-aroylbenzofuran derivatives by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The most intense fragment ions were acylium m/z 105 and [M+H-C6 H6 ]+ , which originated directly from precursor ion as result 2 competitive hydrogen rearrangements. Eliminations CO CO2 also common processes to all analyzed compounds. In addition, eliminations radicals •Br •Cl diagnostic for halogen atoms at aromatic ring A, whereas...

We have evaluated the antischistosomal activity of synthetic dihydrobenzofuran neolignans (DBNs) derived from (±)‐ trans ‐dehydrodicoumaric acid dimethyl ester ( 1 ) and ‐dehydrodiferulic 2 against adult Schistosoma mansoni worms in vitro . Compound 4 ((±)‐ ‐4‐ O ‐acetyldehydrodiferulic ester) displayed most promising activity; at 200 μ m , it kills 100 ± 0% after 24 h, which resembles result achieved with praziquantel (positive control) 1.56 The hydrogenation double bond between C7′ C8′,...

We have investigated the gas-phase fragmentation reactions of eleven synthetic 4-aryl-3,4-dihydrocoumarins by electrospray ionization tandem mass spectrometry (ESI-MS/MS) on a quadrupole-time-of flight (Q-TOF) hybrid spectrometer. also estimated thermochemical data for protonated coumarins (precursor ion A) and product structures computational chemistry at B3LYP level theory to establish rationalize pathways. The most intense peaks in spectra 1–11 resulted from C8H8O2, CO2, C4H4O3, C8H10O3,...

We investigate the chemical composition and in vitro antileishmanial cytotoxic activities of essential oils extracted from leaves Ocotea dispersa (Nees) Mez (OD-EO) odorifera (Vell) Rohwer (OO-EO). On basis GC-FID GC-MS, α-eudesmol (20.9%), valencene (10.2%), δ-elemene (9.3%) isospathulenol (7.3%) are major constituents OD-EO, whereas safrole (36.3%), γ-cadinene (6.6%), camphor (6.5%) α-copaene (6.0%) main OO-EO. Both OD-EO OO-EO display significant activity against promastigote forms...

Abstract We have evaluated the antimicrobial activity of dihydrobenzofuran neolignans (DBNs) 1 ((±)‐ trans ‐dehydrodicoumarate dimethyl ester), 2 ‐dehydrodiferulate ester, 3 ((±)‐4‐ O ‐acetyl‐ and 4 ester) in terms their minimum inhibitory concentration (MIC) values. also assessed cytotoxicity compounds 1–4 to V79 cells by XTT assay. Compounds , display moderate against Streptococcus mitis (MIC=400 μg/ml); compound is active Lactobacillus casei μg/ml). Additionally, moderately Aspergillus...

Hyptis marrubioides Epling is a native plant from Brazilian Cerrado. In this paper, the response of in vitro microplants species to inoculation with bacterial and fungal endophytic isolates evaluated. HPLC-DAD analysis showed presence 3,4-O-(Z)-dicaffeoylquinic acid quercetin-7-O-glucoside as main components. GC/MS demonstrated that sesquiterpenes τ-cadinol caryophyllene oxide were only produced inoculated bacteria, while methyl hexadecanoate, heptadecanoate (Z,Z,Z) 9,12,15-octadecatrienoate...

We investigated the fragmentation of eudesmanolide-type sesquiterpene lactones 1α-(4-hydroxymethacryloyloxy)-3α,4α-epoxy-8α-hydroxyeudesm-11(13)-6α,12-olide (1) and 1α-(2,3-epoxyangeloyloxy)-4α,15-epoxy-8α-hydroxyeudesm-11(13)-6α,12-olide (2) by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The elimination different ester substituent at C(1) directly from protonated 1 2 (A) led to formation two regioisomer product ions B (A - RCO2H). Further resulted consecutive eliminations...

In this work we present a complete proton ( 1 H) and carbon 13 C) nuclear magnetic resonance (NMR) spectral analysis of two synthetic dihydrofuran neolignans (±)-trans-dehydrodicoumarate dimethyl ester (±)-trans-dehydrodiferulate ester.Unequivocal assignments were achieved by H NMR, decoupled C C{ H}) NMR spectra, gradient-selected correlation spectroscopy (gCOSY), J-resolved, heteronuclear multiple quantum coherence (gHMQC), bond (gHMBC) Overhauser effect (NOESY) experiments.All hydrogen...