A5016829021- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Supramolecular Self-Assembly in Materials
- Retinal Imaging and Analysis
- Polydiacetylene-based materials and applications
- Retinal and Optic Conditions
- Advanced Polymer Synthesis and Characterization
- Corneal Surgery and Treatments
- Retinal and Macular Surgery
- Liquid Crystal Research Advancements
- Mesenchymal stem cell research
- Glaucoma and retinal disorders
- Electrospun Nanofibers in Biomedical Applications
- Virus-based gene therapy research
- Tissue Engineering and Regenerative Medicine
- Advanced Glycation End Products research
- Ubiquitin and proteasome pathways
- Ocular Diseases and Behçet’s Syndrome
- Ocular Disorders and Treatments
- CRISPR and Genetic Engineering
- Dendrimers and Hyperbranched Polymers
- Advanced machining processes and optimization
- Block Copolymer Self-Assembly
- Innovations in Education and Learning Technologies
- Animal Vocal Communication and Behavior
He University
2018-2025
He Eye Hospital
2011-2025
Yanbian University
2013-2024
Wageningen University & Research
2023
Changchun Institute of Technology
2022
China Medical University
2018-2020
Doheny Eye Institute
2015
Retinitis pigmentosa (RP) is a type of inherited retinal degeneration (IRD) that typically leads to vision loss in individuals working age. Currently, over 100 genes and loci, as well 1000 individual variants, have been identified relation RP. The aim this study was investigate the genetic distribution characteristics Chinese patients with RP, describe analyze features high-frequency variant from RPGR gene. A total 69 diagnosed RP 36 families were included study. Blood samples collected, DNA...
The purpose of this study was to evaluate expression methyl-CpG-binding protein 2 (MeCP2) in epiretinal membranes from patients with proliferative vitreoretinopathy (PVR) and investigate effects inhibition MeCP2 DNA methylation on transforming growth factor (TGF)-β-induced retinal pigment epithelial (RPE) cell transdifferentiation.Expression its colocalization cytokeratin α-smooth muscle actin (α-SMA) surgically excised PVR studied using immunohistochemistry. 5-AZA-2'-deoxycytidine...
As part of plans to provide help people in remote and poor areas who have no medical resources, a portable slit-lamp based on smartphone was proposed. This would early screening cataract diseases.This means microlens is designed that work with phone's camera. The photo taking function used capturing the image eyes lens replace observation system desktop slit-lamp. A simplified slit light band designed. In order for source meet requirements slit-lamp, adjustable diffused functions ligaments...
Purpose: The purpose of this study was to describe genotype-phenotype associations and novel insights into genetic characteristics in a trio-based cohort inherited eye diseases (IEDs). Methods: To determine the etiological role de novo mutations (DNMs) profile IEDs, we retrospectively reviewed large proband-parent trios Chinese origin. patients underwent detailed examination clinically diagnosed by an ophthalmologist. Panel-based targeted exome sequencing performed on DNA extracted from...
BACKGROUND:Retinal degeneration causes irreversible blindness. Human retinal progenitor cells (hRPCs) have the potential to treat diseases. The vitreous cavity is a relatively immune-privileged site that suitable for stem cell transplantation in treatment of This study aimed evaluate therapeutic efficacy and safety intravitreal injection hRPCs therapy. MATERIAL AND METHODS:hRPCs were primary-cultured injected into RCS rats. To determine whether formed teratomas immune-deficient mice, at...
Abstract Liver cancer is a common malignant tumor worldwide, traditional Chinese medicine one of the treatment measures for liver because its good anti-tumor effects and fewer toxic side effects. Ginsenoside CK (CK) an active component ginseng. This study explored mechanism by which induced ferroptosis in cells. We found that inhibited proliferation HepG2 SK-Hep-1 cells, Ferrostatin-1, inhibitor, was used to verify role inducing Network pharmacological analysis identified FOXO pathway as...
ABSTRACT T‐shaped coil–rod–coil oligomers, consisting of a dibenzo[a,c]phenazine unit and phenyl groups linked together with acetylenyl bonds at the 2,7‐position as rigid segment have been synthesized. The coil segments these new molecules composed poly(ethylene oxide) (PEO)–poly(propylene (PPO) incorporating lateral methyl between rod two flexible alkyl connecting 4,6‐position dibenzo[a,c]phenazine, respectively. experimental results reveal that length PEO chain influence construction...
Human umbilical cord mesenchymal stem cells (hUC-MSCs) have considerable potential in cell therapy. Cryopreservation represents the gold standard storage, but its effect on hUC-MSCs is still not well understood. The aim of this study was to investigate one year cryopreservation and thawing biological characteristics from same donors. Fresh were cryopreserved commercial freezing medium (serum-free CellBanker 2) at passage 2. After cryopreservation, thawed subcultured 4. comparison performed...
Human eyelid adipose-derived stem cells (HEASCs) are a new source of autologous mesenchymal cells, which derived from neuroectoderm and potentially applied in the tissue regeneration cell therapies. Based on prevalence blepharoplasty Asia availability HEASCs, we investigated effect donor age their characteristics regenerative potential HEASCs vitro. The were isolated patients three groups: (1) <20 years (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"...
Panel-based targeted exome sequencing was used to analyze the genetic and clinical findings of genes in a cohort northeast Chinese with retinitis pigmentosa.A total 87 subjects, comprising 23 probands their family members (total patients: 32) confirmed pigmentosa were recruited study. sequence patients members, all subjects underwent complete ophthalmologic examination.Of probands, manifestations include night blindness, narrowing vision, secondary cataracts, choroidal atrophy, color high...
ABSTRACT A new class of π‐conjugated, skewed H‐shaped oligomers, consisting biphenyl, phenylene vinylene, and ethynylene units as the rigid segment, were synthesized via Sonogashira coupling Wittig reactions. The coil segments these molecules composed poly(ethylene oxide) (PEO) or PEO with lateral methyl groups between rod respectively. experimental results revealed that attached to surface dramatically influenced self‐assembling behavior in crystalline phase. rod–coil containing a group at...
The n-shaped rod–coil molecules consisting of an anthracene unit and two biphenyl groups connected by acetylenyl bonds as a conjugated rod segment dendritic poly(ethylene oxide)s with different cross-sectional areas were synthesised. These new molecular structures characterised using 1H NMR MALDI-TOF-MS. self-assembly these in the bulk state aqueous solution was investigated differential scanning calorimetry, X-ray diffraction transmission electron microscopy (TEM). In state, molecule 1a...
Abstract Background Familial exudative vitreoretinopathy (FEVR) is a severe clinically and genetically heterogeneous retinal disorder characterized with failure of vascular development the peripheral retina. The symptoms FEVR vary widely among patients in same family, even between two eyes given patient. purpose this study was to investigate molecular mechanisms by which start codon mutation TSPAN12 causes difference clinical manifestations individuals family. Methods Next‐generation...
The milling of a thin-walled part easily induces deflection in the zones thin walls and floors, where stiffness is not sufficiently high. This problem often leads to inaccuracy geometry roughness. paper proposes supporting mechanism based on Tricept parallel for enhancing work-piece. To fulfill requirements distribution whole work space, redundant actuation introduced mechanism. inverse kinematic model are established. And position branch with optimized algorithm self-adaptive differential...
To characterize the genetic landscape and mutation spectrum of patients with corneal dystrophies (CDs) in a large Han ethnic Chinese Cohort inherited eye diseases (IEDs). Retrospective study. A IED cohort was recruited this study, including 69 clinically diagnosed CD patients, as well other types healthy family members controls. The 792 genes on Target_Eye_792_V2 chip were used to screen all common IEDs our studies, 22 CD-related genes. We identified 2334 distinct high-quality variants...
Abstract Background Panel‐based targeted exome sequencing was applied to identify the pathogenic variants and genetic characteristics of retinitis pigmentosa (RP) in two Chinese families, gain a deeper understanding relationship between clinical manifestations genotypes. Methods A total 17 subjects, comprising probands (total patients: four subjects) their family member, were recruited this study. All subjects underwent comprehensive ophthalmic examinations evaluations, complete history...
In this paper, we report the synthesis and self-assembly behavior of coil-rod-coil molecules, consisting three biphenyls linked through a vinylene unit as conjugated rod segment poly(ethylene oxide) (PEO) with degree polymerization (DP) 7, 12 17, incorporating lateral methyl groups between coil segments segment. Self-organized investigation these molecules by means differential scanning calorimetry (DSC), thermal polarized optical microscopy (POM) X-ray diffraction (XRD) reveals that...
Adeno-associated virus (AAV) vectors have been widely used in therapy to treat hereditary retinal diseases. But its transduction efficiency by intravitreal injection still needs be improved. In this study, we investigated the of AAV-DJ (K137R)-GFP different cells normal mice, as well effection (K137R)-Rs1 on function and structure Rs1-KO mice. The demonstrated that vector transduced all layers retina, including inner nuclear layer photoreceptor layer. found 3 months post-injection improved...
Abstract Background Marfan syndrome (MFS) is a hereditary connective tissue disorder involving multiple systems, including ophthalmologic abnormalities. Most cases are due to heterozygous mutations in the fibrillin‐1 gene (FBN1). Other associated genes include LTBP2, MYH11, MYLK, and SLC2A10. There significant clinical overlap between MFS other Marfan‐like disorders. Purpose To expand mutation spectrum of FBN1 validate pathogenicity Marfan‐related patients with ocular manifestations. Methods...
Human epidermal cell sheet (human-ECS) is a feasible treatment option for wound injury. Traditionally, researchers often use murine 3T3 fibroblast cells as feeder layer to support human grafts, thus increase risk deliver animal-borne infection. To overcome the potential risks involved with xenotransplantation, we develop foreskin culture system and investigate effects of human-ECS on second-degree burn healing in mini-pig order more effective safer therapies enhance human.Human keratinocytes...