Human-associated archaea remain understudied in the field of microbiome research, although particular methanogenic were found to be regular commensals human gut, where they represent keystone species metabolic processes. Knowledge on abundance and diversity human-associated is extremely limited, little known about their function(s), overall role health, or association with parts body other than gastrointestinal tract oral cavity. Currently, methodological issues impede full assessment...

Due to their fundamentally different biology, archaea are consistently overlooked in conventional microbiome surveys. Using amplicon sequencing, we evaluated methodological set-ups detect samples from five body sites: respiratory tract (nasal cavity), digestive (mouth, appendix, and stool) skin. With optimized protocols, the detection of archaeal ribosomal sequence variants (RSVs) was increased one (found currently used, so-called "universal" approach) 81 RSVs a representative sample set....

Forty years ago, archaea were described as a separate domain of life, distinct from bacteria and eukarya. Although it is known for quite long time that methanogenic are substantial components the human gastrointestinal tract (GIT) oral cavity, knowledge on archaeome very limited. Various methodological problems contribute to invisibility archaeome, resulting in severe gaps contradictory information. Similar bacteriome, archaeal biogeography was found be site-specific, forming (i)...

The skin microbiota plays a prominent role in health and disease; however, its contribution to tumorigenesis is not well understood. We comparatively assessed the microbial community compositions from excision specimens of main human non-melanoma cancers, actinic keratosis (AK), squamous cell carcinoma (SCC) basal (BCC). Keratinocyte tumors are characterized by significantly different compositions, wherein AK SCC more similar each other than BCC. Notably, SCC, which represents advanced tumor...

The microbial ecosystem of women undergoes enormous changes during pregnancy and the perinatal period. Little is known about extent in maternal microbiome beyond vaginal cavity its recovery after birth. In this study, we followed pregnant [maternal prepartum (mpre),

It is unknown to what extent the microbiome of preterm infants influenced by hospital regimens including use different probiotics when it comes prevention necrotizing enterocolitis (NEC). Prospective controlled multicenter cohort study very low birth weight from three neonatal intensive care units (NICUs) between October 2015 and March 2017. During this time span, stool was sampled every other day during first two weeks samples were subjected amplicon-based analyses. Out these, seventeen...

In this study, we investigated the biomineralization potential and diversity of epilithic bacterial communities dwelling on limestone statue Saint Donatus, oldest public monument Cluj-Napoca city (Transylvania region, NW Romania). Their spatial distribution together with phylogenetic metabolic diversity, as well their capacity to precipitate calcium carbonate was evaluated by combining molecular phenotypic fingerprinting methods X-ray diffraction, Fourier transform infrared spectroscopy,...

A healthy human microbiome relies on the interaction with and exchange of microbes that takes place between body its environment. People in high-income countries spend most their time indoors for this reason, built environment (BE) might represent a potent source commensal microbes. Anaerobic are particular interest, as researchers have not yet sufficiently clarified how acquires oxygen-sensitive We sampled bathrooms ten households used propidium monoazide (PMA) to assess viability collected...

The causes for preterm birth (PTB) often remain elusive. We investigated whether circulating human milk oligosaccharides (HMOs) might be involved in modulating urinary and vaginal microbiome promoting or preventing PTB. identified here HMOs key microbial taxa associated with indicators of Based on our results, we propose two models how modulate risk PTB: (i) by changes sterile inflammation (microbiome-independent) (ii) HMO-driven shifts (microbiome-dependent). Our findings will guide current...

Present-day terrestrial analogue sites are crucial ground truth proxies for studying life in geochemical conditions close to those assumed be present on early Earth or inferred exist other celestial bodies (e.g. Mars, Europa). Although hypersaline sapropels border-of-life habitats with moderate occurrence, their microbiological and physicochemical characterization lags behind. Here, we study the diversity of under low water activity by describing prokaryotic communities from two disparate...

Dating from the Middle Miocene, massive halite deposits lying beneath Transylvanian Basin (Central Romania) have been valuable mineral resources quarried for millennia. Among numerous hypersaline pit lakes that resulted this mining, Brâncoveanu Lake is unique by its extreme salinity. Assessment of physicochemical variables, water chemistry and trophic status indicated a permanently stratified, pH-neutral, NaCl-rich eutrophied system. We investigated abundance, molecular diversity vertical...

Abstract Due to their fundamentally different biology, archaea are consistently overlooked in conventional microbiome surveys. Using amplicon sequencing, we evaluated methodological set-ups detect samples from five body sites: respiratory tract (nose), digestive (mouth, appendix, and stool) skin. With the optimized protocols, detection of archaeal ribosomal sequence variants (RSVs) was increased one (found currently used, so-called “universal” approach) 81 RSVs a representative sample set....

Recent research provides new insights into the early establishment of infant gut microbiome, emphasizing influence breastfeeding on development gastrointestinal (GIT) microbiomes. In our study, we longitudinally examined taxonomic and functional dynamics oral GIT microbiomes healthy infants (n=30) in their first year, focusing often over-looked aspects, archaeal anaerobic Breastfed (BF) exhibit a more defined transitional phase microbiome compared to non-breastfed (NBF) infants, marked by...

ABSTRACT Recent research provides new insights into the early establishment of infant gut microbiome, emphasizing influence breastfeeding on development gastrointestinal microbiomes. In our study, we longitudinally examined taxonomic and functional dynamics oral tract (GIT) microbiomes healthy infants ( n = 30) in their first year, focusing often-over-looked aspects, archaeal anaerobic Breastfed (BF) exhibit a more defined transitional phase microbiome compared to non-breastfed (NBF)...

Abstract Background Preterm birth is one of the leading causes neonatal mortality. The for spontaneous preterm (PTB) are multifactorial and remain often unknown. In this study, we tested hypothesis that human milk oligosaccharides (HMOs) in blood urine modulate maternal urinary vaginal microbiome influence risk PTB. We analyzed a cross-sectional cohort women with without labor correlated our findings measurements metabolites HMOs blood. Results identified several microbial signatures...

Abstract Background People in westernised countries spend most of their time indoors. A healthy human microbiome relies on the interaction with and exchange microbes that takes place between body its environment. For this reason, built environment might represent a potent source commensal microbes. Anaerobic are particular interest, as researchers have not yet sufficiently clarified how acquires oxygen-sensitive microbes, such obligate or facultative anaerobes. Methods We sampled ten...

Abstract The microbial ecosystem of women undergoes enormous changes during pregnancy and the perinatal period. Little is known about extent in maternal microbiome beyond vaginal cavity its recovery after birth. In this study, we followed pregnant (mpre, n = 30) into postpartum period (1 month postpartum, mpost, 30). We profiled their oral, urinary, microbiome, archaeome, mycobiome urinary metabolome compared them with nonpregnant (np, 29). Overall, status mpre, mpost) had a smaller effect...

Das menschliche Darmmikrobiom ist ein komplexes Ökosystem, in dem Milliarden von Mikroorganismen koexistieren. Es Bakterien dominiert, umfasst aber auch Archaeen, Pilze, Protozoen, Eukaryoten und Viren. Am häufigsten sind die beiden bakteriellen Phylotypen Bacteroides Firmicutes. Die anderen Stämme, Proteobakterien, Actinobakterien, Fusobakterien Verrucomicrobia, vergleichsweise weniger häufig [11]. Zusammensetzung des Darmmikrobioms weist große individuelle Unterschiede auf wird durch viele...

The gut microbiome has a major impact on human health. Dysbiosis can contribute to various mental disturbances via the gut-brain axis [1], such as depression, for which St. John's wort is effective. (Hypericum perforatum L.) contains 2 – 4% quercetin glycosides [2]. These are metabolized in gut. [3]. aim of study was determine whether owes its beneficial effect by affecting microbiome. A literature survey therefore conducted according PRISMA 'quercetin AND microbiome'.

The root of marshmallow (Althaea officinalis L.) is commonly known for its anti-inflammatory action and used traditionally in different inflammatory conditions like sore throat. Furthermore, there are some hints traditional, topical usage on inflamed skin [1], [2]. aim this study was to investigate the influence extract gene expression proinflammatory cytokines further markers active AD lesions by using a 3D model atopic dermatitis (AD) [3].